Renalase mRNA levels in the brain, heart, and kidneys of spontaneously hypertensive rats with moderate and high hypertension

Abstract

BackgroundRenalase is a recently discovered secretory protein involved in regulation of arterial blood pressure in humans and animals. Results of animal experiments from independent laboratories indicate that administration of human recombinant renalase decreases blood pressure and some genetically predisposed hypertensive rats have lowered renalase levels.Material/MethodsThe levels of renalase mRNA expression in brain hemispheres, heart, and kidneys of spontaneously hypertensive rats (SHR) with moderate (140–180 mm Hg) or high (>180 mm Hg) hypertension and of control Wistar-Kyoto (WKY) rats were analyzed using real-time PCR.ResultsSpontaneously hypertensive rats with high hypertension (>180 mm Hg) had a lower renalase mRNA level in brain hemispheres, and higher heart and kidney renalase mRNA levels compared with control WKY rats. In SHR with a moderate increase in arterial blood pressure (140–180 mm Hg), the tissue renalase mRNA changed in the same direction but did not reach the level of statistical significance as compared with control rats.ConclusionsThe results indicate that the development of hypertension in SHR is accompanied by altered expression of the renalase gene in the examined organs as compared with control WKY rats. The brain and peripheral tissues renalase mRNA levels demonstrate opposite trends, which are obviously crucial for impaired regulation of blood pressure in SHR.

Effects of Renin-Angiotensin System Inhibitors on Renal Expression of Renalase in Sprague-Dawley Rats Fed With High Salt Diet

“…Our family-based association study identified several single-nucleotide polymorphisms of the renalase gene, namely, rs919115, rs792205, and rs12356177, which were associated with BP responses to changes in dietary salt intake [18]. In a study by Fedchenko et al [24] that involved SHR, the kidney renalase mRNA in rats with a moderate increase in BP (140 mm Hg) did not change as much as that in the control rats. In the current study, we observed pronounced decreases in renal and circulating renalase levels in the HS and HS+H groups, which indicated that high salt intake, independent of BP, decreased the renalase expression in the SD rats.…”

Renalase Gene Polymorphism in Patients with Hypertension and Concomitant Coronary Heart Disease

“…Renalase is involved in the regulation of blood pressure and cardiac function via degrading catecholamines in the blood circulation [3,4]. Human renalase gene is mapped to 10q23.33 [5] and highly expressed in the kidney and heart [6,7]. To date, available studies focus on the role of renalase gene polymorphism in the hypertension, and the single nucleotide polymorphisms (SNP) studied in previous studies is usually localized at the potential functional domains.…”

Renalase — a new marker or just a bystander in cardiovascular disease: clinical and experimental data

“…They observed lower mRNA renalase in brain hemisphere and higher kidney and heart renalase mRNA levels in hypertensive rats (RR systolic > 180 mm Hg) in comparison to rats from the control group. The authors concluded that peripheral and brain renalase mRNA levels change in the opposite directions in hypertensive rats [14].…”

“…The same effect was observed after administration of 5 mg/kg enalapril. Fedchenko et al [14] analysed, with the use of real-time polymerase chain reaction, the levels of renalase mRNA expression in brain, heart, and kidneys of spontaneously hypertensive rats. They observed lower mRNA renalase in brain hemisphere and higher kidney and heart renalase mRNA levels in hypertensive rats (RR systolic > 180 mm Hg) in comparison to rats from the control group.…”