Renal transplantation in the rat ? A new simple, non-suture technique

Savas, C. P.; Nolan, M. S.; Lindsey, N. J.; Boyle, P. F.; Slater, David; Fox, Miles

doi:10.1007/bf00261573

Cited by 16 publications

(10 citation statements)

References 3 publications

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“…23 Combinations using Lewis to Sprague-Dawley, 16,18 Lewis to DA-rats, 24,25 and Lewis to Fisher rats 34 were also reported.…”

Section: Selection and Handling Of Animalsmentioning

confidence: 84%

“…Savas et al described a nonsuturing technique using small polyethylene cuffs for anastomosing the renal vessels, and reported a considerably reduced warm ischemic time (mean, 12 min). 23 Ureter anastomosis. Ureter anastomosis is possibly the most difficult part of kidney transplantation in the rat.…”

Section: End-to-side Techniquementioning

confidence: 99%

“…The blood supply of the transplanted ureter will rely on blood from the kidney and therefore from the small periureteral nourishing vessels. In a third of the studies, the end-to-end procedure was used, 16,18,19,22,24À27,35 in a third a bladder-patch technique, 6À9,12,15,17,29 and in the remaining third, either the direct insertion of the ureter into the bladder 1,11,28,30,31,33,34 or placement of a stent or cannulation of the ureter 5,14,16,21,23,32 were performed equally often.…”

Section: End-to-side Techniquementioning

confidence: 99%

“…Polyethylene stents were inserted into the donor and recipient ureter and secured either by circumferential sutures with a nylon or silk, 33 or by putting a ligature near the divided ends of the ureters. 23 For the end-to-end ureterostomy, the use of an indwelling reabsorbable ureteral stent of 5-0 plain catgut was used. 21 The anastomosis is completed with a 11-0 Vicryl, using 6À8 interrupted sutures.…”

Section: End-to-side Techniquementioning

confidence: 99%

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Kidney transplantation in rats: An appraisal of surgical techniques and outcome

2003

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Renal transplantation in rats is an essential experimental tool in transplantation research. The surgical procedure per se could affect the outcome of an experiment, independent of the hypothesis addressed, therefore requiring a standardized method which should be comparable across studies. To date, however, there is little information on the optimal surgical technique. We performed a Medline search on original articles published between 1965-2001 in order to evaluate whether specific technical issues affecting the outcome of the procedure could be defined. Articles that reported on a novel microsurgical procedure, or whose main purpose was the outcome of a surgical technique itself, were included in the analysis. From 2,060 retrieved publications, 34 corresponded to the selection criteria (rats and microsurgery and technique and kidney or renal transplantation). Among the essential determining factors for a good outcome, body weight >200 g and warm ischemic time <30 min were identified. Other important factors were the techniques used for vascular (end-to-end and end-to-side procedure or sleeve technique) and ureteral (bladder patch or end-to-end procedure) anastomosis. Gender, animal strain, type of anesthesia, prophylactic administration of antibiotics, and type of flushing solution did not affect the success of renal allografts. In order to avoid a bias related to the surgical procedure in rat renal transplantation, a warm ischemia time <30 min in animals with a body weight >200 g seems to be essential. Also, end-to-end or end-to-side vascular anastomoses are preferable to the sleeve technique. Other factors do not influence the immediate function of the graft.

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“…23 Combinations using Lewis to Sprague-Dawley, 16,18 Lewis to DA-rats, 24,25 and Lewis to Fisher rats 34 were also reported.…”

Section: Selection and Handling Of Animalsmentioning

confidence: 84%

Section: End-to-side Techniquementioning

confidence: 99%

Section: End-to-side Techniquementioning

confidence: 99%

Section: End-to-side Techniquementioning

confidence: 99%

See 2 more Smart Citations

Kidney transplantation in rats: An appraisal of surgical techniques and outcome

2003

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“…To obviate these problems, Kamada 29 and Savas and associates 30 described a nonsuturing technique (cuff technique) using small polyethylene cuffs for anastomosing the renal vessels and reported considerable reduction of warm ischemia time (mean of 12 min) (Figure 3e). …”

Section: Cuff Techniquementioning

confidence: 99%

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Shrestha

Haylor²

2017

Exp Clin Transplant

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Microvascular surgical techniques of renal transplant in rats have evolved over the past 5 decades to achieve successful rat renal transplant; these modifications have included surgical techniques to address the anatomic variations in the renal blood vessels and those to reduce ischemic and operation durations. Here, we review the surgical techniques of renal transplant in rats and evaluate the advantages and disadvantages of individual techniques of vascular and ureteric anastomoses. For this review, we performed a systematic literature search using relevant medical subject heading terms and included appropriate publications in the review. Since the first description of a rat model of renal transplant by Bernard Fisher and his colleagues in 1965, which used end-to-side anastomosis between the renal vein and renal artery to the recipient inferior vena cava and aorta, several vascular and ureteric anastomosis techniques have been modified. Vascular anastomosis techniques now include end-to-end anastomosis, use of donor aortic and inferior vena cava conduits, sleeve and cuff anastomoses, and application of fibrin glue. Likewise, restoration of the urinary tract can now be achieved by direct anastomosis of the donor ureter to the recipient bladder, end-to-end anastomosis between the donor and recipient ureters, and donor bladder cuff to the recipient bladder. There are advantages and disadvantages attributable to individual techniques. The range of vascular and ureteric anastomosis techniques that has emerged reflects the need for mastering more than one technique to suit the vascular anatomy of individual animals and to reduce operating time for achieving successful outcomes after renal transplant.

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Kidney transplantation procedures in rats: Assessments, complications, and management

et al. 2006

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Kidney transplantation in rats is an experimental model often used for the development of general microsurgical or transplantation techniques, for immunologic studies, and for analyzing transplant-associated long-term arterial blood-pressure changes. The aim of the present study was to analyze different surgical techniques of kidney transplantation in rats, with emphasis on minimizing surgical complications and establishing guidelines for their prevention and management. Complications were categorized into general (e.g., core body temperature drop, ischemic time) and surgically related vascular and urinary tract complications. In conclusion, a significant reduction of the complication rate in renal transplantation in rats can be achieved by placing the animal on a heating pad at an appropriate temperature. To reduce the risk of vascular thrombosis, ice-cold saline with heparin and careful flushing of the donor kidneys are recommended. Vascular complications can be avoided by performing "end-to-end" anastomosis techniques. The use of stents and cannulas in the urinary tract is associated with a high risk of urinary tract obstruction, and therefore is not recommended.

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Renal transplantation in the rat ? A new simple, non-suture technique

Abstract: Renal transplantation in the rat can be performed by a simple technique utilising cuff anastomosis. This method is quick and reliable, and the results compare well with those achieved by standard microsurgical techniques.

Cited by 16 publications

References 3 publications

Kidney transplantation in rats: An appraisal of surgical techniques and outcome

Kidney transplantation in rats: An appraisal of surgical techniques and outcome

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Kidney transplantation procedures in rats: Assessments, complications, and management

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