Renal micro<scp>RNA</scp>‐ and <scp>RNA</scp>‐profiles in progressive chronic kidney disease

Rudnicki, Michael A.; Perco, Paul; D’haene, Barbara; Leierer, Johannes; Heinzel, Andreas; Mühlberger, Irmgard; Schweibert, Ninella; Sunzenauer, Judith; Regele, Heinz; Kronbichler, Andreas; Mestdagh, Pieter; Vandesompele, Jo; Mayer, Bernd; Mayer, Gert

doi:10.1111/eci.12585

Cited by 99 publications

(73 citation statements)

References 54 publications

(67 reference statements)

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“…CCL19 has been reported to be expressed by cultured podocytes and kidneys suffering from chronic glomerular diseases (65,66). Given the acuity of our model, it is unlikely the effect we noted on renal function following DT therapy resulted from depletion of CCL19-expressing cells of normal kidneys.…”

Section: Discussionmentioning

confidence: 87%

Repetitive ischemic injuries to the kidneys result in lymph node fibrosis and impaired healing

Maarouf¹,

Uehara²,

Kasinath³

et al. 2018

JCI Insight

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Section: Discussionmentioning

confidence: 87%

Repetitive ischemic injuries to the kidneys result in lymph node fibrosis and impaired healing

Maarouf¹,

Uehara²,

Kasinath³

et al. 2018

JCI Insight

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“…miR-22 was found down-regulated in clear cell renal cell carcinoma (RCR) samples when compared to adjacent non-tumorous samples (He et al , 2015). The expression of miR-128 was reported also as down-regulated in the kidneys of patients with progressive versus stable chronic kidney disease (Rudnicki et al , 2016). In vitro studies in human embryonic kidney cells (HEK293T) suggest an anti-proliferative role for miR-128 through apoptosis regulation (Adlakha and Saini, 2011).…”

Section: Discussionmentioning

confidence: 99%

Effects of a 28-day dietary co-exposure to melamine and cyanuric acid on the levels of serum microRNAs in male and female Fisher 344 rats

Silva

Chang

Williams

et al. 2016

Food and Chemical Toxicology

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We showed previously that a 28-day combined dietary exposure to melamine and cyanuric acid (MEL&CYA) induced kidney lesions in NCTR Fisher 344 (F344) rats. Histopathological changes were significant in females dosed with ≥240ppm MEL&CYA and in males dosed with ≥180ppm MEL&CYA; however, the nephrotoxicity biomarkers blood urea nitrogen (BUN) and serum creatinine (SCr) were increased only by ≥240ppm MEL&CYA. The serum miRNome has been reported to reflect toxicity of several organs, including the kidney. Here, we compared the dose-response of alterations in serum miRNAs to those of BUN, SCr, and kidney histopathology in rats co-exposed to MEL&CYA. The serum miRNome of male F344 rats dosed with 0, 180, or 240ppm MEL&CYA was screened using quantitative real-time RT-PCR (qRT-PCR) and the levels of selected serum miRNAs were analyzed further in both sexes over the full dose range. The levels of several miRNAs were significantly reduced in rats treated with 240ppm MEL&CYA versus control. In addition, miR-128-3p and miR-210-3p were decreased in males treated with 180pm MEL&CYA, a dose at which the levels of BUN and SCr were not yet affected by treatment. These data suggest that the serum miRNome is affected by nephrotoxic doses of MEL&CYA in male and female rats.

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“…miR-30a-3p was decreased in acute cellular rejection50. miR-30d-5p was down-regulated in progressive kidney disease from diabetic and/or hypertensive nephropathy51. TGF-ß treatment of podocytes in vitro resulted in a diminished expression of all miR-30 family members52.…”

Section: Discussionmentioning

confidence: 99%

Comparison of different normalization strategies for the analysis of glomerular microRNAs in IgA nephropathy

Bockmeyer

Säuberlich

Wittig

et al. 2016

Sci Rep

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Small nucleolar RNAs (snoRNAs) have been used for normalization in glomerular microRNA (miRNA) quantification without confirmation of validity. Our aim was to identify glomerular reference miRNAs in IgA nephropathy. We compared miRNAs in human paraffin-embedded renal biopsies from patients with cellular-crescentic IgA-GN (n = 5; crescentic IgA-GN) and non-crescentic IgA-GN (n = 5; IgA-GN) to mild interstitial nephritis without glomerular abnormalities (controls, n = 5). Laser-microdissected glomeruli were used for expression profiling of 762 miRNAs by low-density TaqMan arrays (cards A and B). The comparison of different normalization methods (GeNormPlus, NormFinder, global mean and snoRNAs) in crescentic IgA-GN, IgA-GN and controls yielded similar results. However, levels of significance and the range of relative expression differed. In median, two normalization methods demonstrated similar results. GeNormPlus and NormFinder gave different top ranked reference miRNAs. Stability ranking for snoRNAs varied between cards A and B. In conclusion, we suggest the geometric mean of the most stable reference miRNAs found in GeNormPlus (miR-26b-5p), NormFinder (miR-28-5p) and snoRNAs (RNU44) as reference. It should be considered that significant differences could be missed using one particular normalization method. As a starting point for glomerular miRNA studies in IgA nephropathy we provide a library of miRNAs.

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Renal microRNA‐ and RNA‐profiles in progressive chronic kidney disease

Abstract: These results suggest the involvement of specific miRNAs and mRNAs in biological pathways associated with the progression of CKD.

Cited by 99 publications

References 54 publications

Repetitive ischemic injuries to the kidneys result in lymph node fibrosis and impaired healing

Repetitive ischemic injuries to the kidneys result in lymph node fibrosis and impaired healing

Effects of a 28-day dietary co-exposure to melamine and cyanuric acid on the levels of serum microRNAs in male and female Fisher 344 rats

Comparison of different normalization strategies for the analysis of glomerular microRNAs in IgA nephropathy

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