Renal Hypertrophy Factor in Serum of Nephrectomized Rats, with Observations on Species Specificity

F, Vichi; Earle, David P.

doi:10.3181/00379727-135-34982

Cited by 11 publications

(6 citation statements)

References 3 publications

(3 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This has led over the years to a large body of work involving the search for a circulating factor, a renotropin, capable of promoting kidney growth (10,17). The approaches used include parabiosis experiments (34,35), injections of sera or plasma from nephrectomized animals to induce in vivo kidney growth (36,37), and testing of similar sera or plasma as mitogenic agents in vitro (38,39). The existence of such a renotropin, however, remains at best controversial (10), and attempts to characterize it have not advanced further than crude fractionation techniques.…”

Section: Discussionmentioning

confidence: 99%

Relative Increase in Insulin-Like Growth Factor I Messenger Ribonucleic Acid Levels in Compensatory Renal Hypertrophy*

1987

View full text Add to dashboard Cite

Immunoreactive insulin-like growth factor I (IGF-I) has recently been demonstrated in multiple tissues, including liver, kidney, lung, testes, and brain. Tissue IGF-I levels in hypophysectomized rats are elevated by GH. To examine whether tissue IGF-I production is regulated by local as well as systemic influences, we studied rat kidney IGF-I gene expression in renal compensatory hypertrophy occurring after unilateral nephrectomy. Northern analysis of kidney poly(A) RNA probed with [32P]IGF-I mouse cDNA revealed the presence of a mRNA species 1.3 kilobases in size. Dot hybridization of kidney poly(A) RNA showed that IGF-I mRNA was induced 5- to 6-fold in the kidneys of nephrectomized animals relative to levels in control sham-operated rats. This induction was present 24 h after surgery and continued for at least 7 days after the operation. Kidney radioimmunoassayable IGF-I content was also increased 73% in nephrectomized animals, although this was only apparent on the fourth day after surgery. In contrast, liver IGF-I mRNA levels were comparable in both experimental and control animals, suggesting that the IGF-I induction was specific to the tissue undergoing compensatory growth. Serum IGF-I and GH levels were not altered in nephrectomized and control animals for the duration of the experiments. These studies, therefore, confirm that IGF-I is synthesized in the kidney, and that kidneys undergoing compensatory growth have increased levels of IGF-I mRNA. This phenomenon occurs independently of changes in GH secretion, indicating that paracrine or autocrine factors are involved in the control of renal IGF-I production.

show abstract

Section: Discussionmentioning

confidence: 99%

Relative Increase in Insulin-Like Growth Factor I Messenger Ribonucleic Acid Levels in Compensatory Renal Hypertrophy*

1987

View full text Add to dashboard Cite

show abstract

“…Subsequently a number of experiments involving parabiotic animals demonstrated that unilateral or bilateral nephrectomy in one animal resulted in renal hypertrophy of the other (Van Vroonhoren, Soler Mountesinos & Malt, 1972;Shames, Murphy & Berkowitz, 1976;Obertop & Malt, 1977;Van Urk, Malamud, Soler Mountesinos & Malt, 1978). Furthermore it has been demonstrated that accelerated DNA synthesis in kidneys of animals followed injection of sera from nephrectomized animals (Lowenstein & Stern, 1963;Vichi & Earle, 1970). Finally, in vitro studies involving incubation of renal cells with sera or plasma from nephrectomized animals have demonstrated stimulation of mitotic index (Ogawa & Nowinski, 1958) or enhancement of protein (Dicker & Morris, 1980) or DNA and RNA synthesis (Preuss & Goldin, 1979;Castillo, Robertson, Goldin & Preuss, 1980).…”

Section: Introductionmentioning

confidence: 99%

The renotrophic factor, a persistent stimulus that crosses the placenta in mice.

Averbukh¹,

Bogin²,

Cohn³

et al. 1988

The Journal of Physiology

View full text Add to dashboard Cite

SUMMARY1. Twenty 7-week female mice underwent right nephrectomy and twenty others were sham operated. A week later all animals were made pregnant. Pregnancy was repeated five more times consecutively and various renal parameters were assessed in the pups.2. Fractional fresh kidney weight (relative to body weight) was significantly increased in the pups of nephrectomized mothers while percentage renal water and protein content expressed as mg/g kidney weight were not statistically different in the two groups of pups. Thus dry kidney weight and amount of protein per kidney were increased in the experimental group. This was true for the newborns of all six pregnancies.3. Renal morphometric studies performed in newborns of first pregnancies showed that the mean number of glomeruli per microscopic field, mean fractional cumulative glomerular area (relative to microscopic field area) and the mean number of cells per glomerulus were significantly greater in the experimental group. Mean glomerular radius was not statistically different in the two groups.4. The results indicate that: (1) the renotrophic factor(s) crosses the placenta in mice; (2) its activity in maternal circulation following uninephrectomy is sustained for a relatively long period; and (3) fetal response to enhanced maternal renotrophin stimulation consists of increased renal dry weight and renal protein, formation of super-physiological numbers of glomeruli and cellular hyperplasia of the glomeruli.

show abstract

“…Royce [18] has shown that chemical peritonitis produced by the placement of talc in the abdominal cavity also will inhibit compensatory growth. Several investigators have demonstrated a serum factor in nephrectomized rats which stimulates renal mitotic activity in normal rats [19], The nature of these factors remains controversial.…”

Section: Renal Growth and Anatomical Developmentmentioning

confidence: 99%

The Developing Kidney

Nash¹,

Edelmann²

1973

Nephron

View full text Add to dashboard Cite

Although the neonatal kidney indeed may be a functionally imperfect organ, intuition, prejudice, and a knowledge of evolution lead us to expect otherwise. The alternative possibilities proposed here are largely unproven but would seem to be a plausible synthesis of renal growth, function and stimulatory factors into an efficient system for the support of optimal growth. Although quantitatively the infant may be limited in his capacity to respond to abnormal stresses, such as in states of disease, it would seem axiomatic that the kidney functionally must be ideally suited to meet the needs of the healthy infant. Most important is the realization that adult levels of glomerular filtration, tubular reabsorption, and ratios between the two may be, and theoretically should be, inappropriate standards for the infant. As research in developmental nephrology and in pediatrics in general moves from a static, descriptive approach to a dynamic, physiological one a multitude of exciting and crucial questions await investigation.

show abstract

Renal Hypertrophy Factor in Serum of Nephrectomized Rats, with Observations on Species Specificity

Cited by 11 publications

References 3 publications

Relative Increase in Insulin-Like Growth Factor I Messenger Ribonucleic Acid Levels in Compensatory Renal Hypertrophy*

Relative Increase in Insulin-Like Growth Factor I Messenger Ribonucleic Acid Levels in Compensatory Renal Hypertrophy*

The renotrophic factor, a persistent stimulus that crosses the placenta in mice.

The Developing Kidney

Contact Info

Product

Resources

About