Renal Excretion of 8-Oxo-7,8-dihydro-2′-deoxyguanosine in Wistar Rats with Increased O2 Consumption Due to Cold Stress

Topp, Heinrich; Lengger, Christoph; Schöch, G.; Werner, J.; Mietzsch, E.

doi:10.1006/abbi.2000.1720

Cited by 12 publications

(9 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cold exposure has been previously used to assess how an increased metabolism may impact ageing in rodents (Holloszy and Smith, 1986;Topp et al, 2000;Selman et al, 2002;Kaushik and Kaur, 2003;Venditti et al, 2004;Selman et al, 2008;Vaanholt et al, 2009). Several studies found significant short-to mid-term effects (from 10 h to 3 weeks) of cold challenge on oxidative stress markers, with, for example, increased oxidative damage (Topp et al, 2000;Selman et al, 2002;Kaushik and Kaur, 2003;Venditti et al, 2004) or tissue-specific modifications of antioxidant defences, which globally reflect a situation of oxidative stress (Kaushik and Kaur, 2003;Venditti et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

“…Several studies found significant short-to mid-term effects (from 10 h to 3 weeks) of cold challenge on oxidative stress markers, with, for example, increased oxidative damage (Topp et al, 2000;Selman et al, 2002;Kaushik and Kaur, 2003;Venditti et al, 2004) or tissue-specific modifications of antioxidant defences, which globally reflect a situation of oxidative stress (Kaushik and Kaur, 2003;Venditti et al, 2004). However, while always inducing a rise in metabolism, mid-to long-term cold challenge experiments produced contrasting results.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Mitochondrial uncoupling prevents cold-induced oxidative stress: a case study using UCP1 knock-out mice

Stier

Bize

Habold

et al. 2013

Journal of Experimental Biology

View full text Add to dashboard Cite

The relationship between metabolism and reactive oxygen species (ROS) production by the mitochondria has often been (wrongly) viewed as straightforward, with increased metabolism leading to higher generation of pro-oxidants. Insights into mitochondrial functioning show that oxygen consumption is principally coupled with either energy conversion as ATP or as heat, depending on whether the ATP-synthase or the mitochondrial uncoupling protein 1 (UCP1) is driving respiration. However, these two processes might greatly differ in terms of oxidative costs. We used a cold challenge to investigate the oxidative stress consequences of an increased metabolism achieved either by the activation of an uncoupled mechanism (i.e. UCP1 activity) in the brown adipose tissue (BAT) of wild-type mice or by ATP-dependent muscular shivering thermogenesis in mice deficient for UCP1. Although both mouse strains increased their metabolism by more than twofold when acclimatised for 4 weeks to moderate cold (12°C), only mice deficient for UCP1 suffered from elevated levels of oxidative stress. When exposed to cold, mice deficient for UCP1 showed an increase of 20.2% in plasmatic reactive oxygen metabolites, 81.8% in muscular oxidized glutathione and 47.1% in muscular protein carbonyls. In contrast, there was no evidence of elevated levels of oxidative stress in the plasma, muscles or BAT of wild-type mice exposed to cold despite a drastic increase in BAT activity. Our study demonstrates differing oxidative costs linked to the functioning of two highly metabolically active organs during thermogenesis, and advises careful consideration of mitochondrial functioning when investigating the links between metabolism and oxidative stress.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Mitochondrial uncoupling prevents cold-induced oxidative stress: a case study using UCP1 knock-out mice

Stier

Bize

Habold

et al. 2013

Journal of Experimental Biology

View full text Add to dashboard Cite

show abstract

“…These facts have led to the use of urinary oxo 8 dG as a noninvasive marker of oxidative DNA damage by ROS in various (patho)physiological and nutritional studies (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14). Recently, we investigated in Wistar rats the effect of an elevated O 2 -consumption by on average 50% over 6 days provoked by cold stress on the renal excretion rate of oxo 8 dG (15). We have developed a HPLC-EC method for the reliable determination of oxo 8 dG in rat urine (15) that is also suitable for human urine.…”

mentioning

confidence: 98%

“…Recently, we investigated in Wistar rats the effect of an elevated O 2 -consumption by on average 50% over 6 days provoked by cold stress on the renal excretion rate of oxo 8 dG (15). We have developed a HPLC-EC method for the reliable determination of oxo 8 dG in rat urine (15) that is also suitable for human urine. We used four HPLCcolumns in series and an automated column-switching technique.…”

mentioning

confidence: 99%

A High-Performance Liquid Chromatographic Method for the Determination of 8-Oxo-7,8-dihydro-2′-deoxyguanosine in Urine from Man and Rat

Lengger

Schöch

Topp

2000

Analytical Biochemistry

Self Cite

View full text Add to dashboard Cite

“…Incidentally, cold-induced thermoregulation is also associated with increased lipid metabolism and is age-dependent (McDonald et al, 2004). Low temperature exposures lead to an increased metabolic rate (Selman et al, 2000), elevated reactive oxygen species (ROS) (Venditti et al, 2004) and OS-induced tissue damage (Topp et al, 2000). It is possible that cold-stimulated high metabolic rates can alter blood lipid profiles and accelerate the development and progression of OS-related occupational hazards such as reduced cognitive performance in terms of memory, vigilance and concentration (Pilcher et al, 2002;Palinkas, 2001).…”

Section: Introductionmentioning

confidence: 99%

Intermittent cold-induced hippocampal oxidative stress is associated with changes in the plasma lipid composition and is modifiable by vitamins C and E in old rats

Devi

Manjula

2014

Neurochemistry International

View full text Add to dashboard Cite

a b s t r a c tThis study primarily investigated the effects of intermittent cold exposure (ICE) on oxidative stress (OS) in the hippocampus(HC) and plasma lipid profile of old male rats. Secondly, it evaluated structural changes in the hippocampus region of the rat's brain. Thirdly, it attempted an evaluation of the effectiveness of the combined supplement of vitamins C and E in alleviating cold stress in terms of these biochemical parameters. Thirty male rats aged 24 months were divided into groups of five each: control (CON), cold-exposed at 10°C (C10), cold-exposed at 5°C (C5), supplemented control (CON+S), and supplemented cold-exposed at either 5°C (C5+S) or 10°C (C10+S). The rats were on a daily supplement of vitamin C and vitamin E. Cold exposure lasted 2 h/day for 4 weeks. Rats showed increased levels of hydrogen peroxide (H 2 O 2 ), and thiobarbituric acid reactive substances (TBARS) in the HC at 10°C with further increase at 5°C. Cold also induced neuronal loss in the hippocampus with concomitant elevations in total cholesterol (TCH), triglycerides (TG) and low-density lipoproteins (LDL-C) levels, and a depletion in high-density lipoprotein (HDL-C). A notable feature was the hyperglycaemic effects of ICE and depleted levels of vitamins C and E in the hippocampus and plasma while supplementation increased their levels. More importantly, a positive correlation was observed between plasmatic LDL-C, TCH and TG and hippocampal TBARS and H 2 O 2 levels. Further, intensity of cold emerged as a significant factor impacting the responses to vitamin C and E supplementation. These results suggest that cold-induced changes in the plasma lipid profile correlate with OS in the hippocampus, and that vitamin C and E together are effective in protecting from metabolic and possible cognitive consequences in the old under cold exposures.

show abstract

Renal Excretion of 8-Oxo-7,8-dihydro-2′-deoxyguanosine in Wistar Rats with Increased O2 Consumption Due to Cold Stress

Cited by 12 publications

References 21 publications

Mitochondrial uncoupling prevents cold-induced oxidative stress: a case study using UCP1 knock-out mice

Mitochondrial uncoupling prevents cold-induced oxidative stress: a case study using UCP1 knock-out mice

A High-Performance Liquid Chromatographic Method for the Determination of 8-Oxo-7,8-dihydro-2′-deoxyguanosine in Urine from Man and Rat

Intermittent cold-induced hippocampal oxidative stress is associated with changes in the plasma lipid composition and is modifiable by vitamins C and E in old rats

Contact Info

Product

Resources

About