Abstract:1. AM 188 is an antiviral guanosine analogue that undergoes extensive renal excretion in humans. The present study was designed to investigate the disposition of AM 188 over a range of concentrations in the rat isolated perfused kidney (IPK) to explore the mechanisms involved in its renal handling. 2. Right kidneys of male Sprague-Dawley rats (n = 23) were isolated and perfused in recirculating mode with Krebs'-Henseleit (pH 7.4) buffer containing 0.65% bovine serum albumin, 3.6% dextran, amino acids and gluco… Show more
“…The utility of the isolated perfused kidney (IPK) with intact filtration capacity (filtering IPK) has been demonstrated in several studies from this and other laboratories. [20][21][22][23] Additionally, the nonfiltering IPK can provide important insights into renal drug and metabolite disposition. The nonfiltering IPK utilises a perfusate that creates high oncotic pressure to counterbalance filtration forces.…”
The data confirmed an important role for the kidney in the metabolic clearance of xenobiotics via glucuronidation and signalled the lack of impact of impaired glomerular filtration on renal drug metabolism.
“…The utility of the isolated perfused kidney (IPK) with intact filtration capacity (filtering IPK) has been demonstrated in several studies from this and other laboratories. [20][21][22][23] Additionally, the nonfiltering IPK can provide important insights into renal drug and metabolite disposition. The nonfiltering IPK utilises a perfusate that creates high oncotic pressure to counterbalance filtration forces.…”
The data confirmed an important role for the kidney in the metabolic clearance of xenobiotics via glucuronidation and signalled the lack of impact of impaired glomerular filtration on renal drug metabolism.
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