Removal of two cytostatic drugs: bleomycin and vincristine by white-rot fungi – a sorption study

Abstract

Purpose Cytostatic drugs cannot be easily removed by conventional sewage treatment plants, resulting in their ultimate release into aquatic systems where they become a threat. Thus, new technologies which can be used to eliminate these drugs more effectively before they enter the environment are increasingly important. Fungal treatment of wastewaters is a promising and environmentally friendly technology for pharmaceutical remediation. The aim of this work is to examine the biosorption of two cytostatics, bleomycin and vincristine, in the aqueous solution by fungal biomass. Methods Five white-rot fungi were used in this study: Fomes fomentarius (CB13), Hypholoma fasciculare (CB15), Phyllotopsis nidulans (CB14), Pleurotus ostreatus (BWPH), and Trametes versicolor (CB8). Tests were conducted on different types of biomass (alive and dead – autoclaved) and in various physico-chemical conditions: varied drug concentrations (5, 10 and 15 mg/L), temperatures (from 15.4 to 29.6 °C), and pH (from 3.2 to 8.8). Results The results showed that among alive biomass, T. versicolor (CB8) had the greatest sorption ability for bleomycin and P. nidulans (CB14) worked best for vincristine. The tested sorption process could be described by a pseudo-second order kinetics model. Sorption equilibrium studies demonstrated that for bleomycin Redlich-Peterson, while for vincristine Langmuir model fitted best. The thermodynamic studies showed that the sorption process was endothermic chemisorption for bleomycin, and exothermic physisorption for vincristine. For both drugs the sorption ability increased with an increase of the pH value. Conclusion The biosorption on fungal biomass is a favorable alternative to conventional wastewater treatment processes for anticancer drug removal.

Green Biotechnology of Oyster Mushroom (Pleurotus ostreatus L.): A Sustainable Strategy for Myco-Remediation and Bio-Fermentation

“…Decabromo-diphenyl ethane (5, 20 mg L −1 ) Biodegradation after 120 h Biodegradation of pollutants by enzyme P450, manganese peroxidase, lignin peroxidase, and laccase [21] Cytostatic drugs include vincristine and bleomycin (5, 10 and 15 mg L −1 ) Cultivated in liquid medium for 30 days before the test Studied drugs as anticancer treatments can be removed by biosorption on fungal biomass during wastewater treatment [87] Sulfonamide antibiotics (0.1 mM) Biodegradation after 14 d in polluted wastewater Mushrooms as biofilters removed sulfonamides by up to 83-91% of the applied doses over 14 d from polluted wastewater [88] Cadmium at doses ranging from 0.5 to 20 mg L −1 Cd Removal rate up to 54.6% for 7 days Cd detoxification pathways included 26 enzymes, including catalase, superoxide dismutase, and peroxisomal enzymes [89] Petroleum hydrocarbons in soils (339 g kg −1 dry weight) for 90 days Mushroom spawn (10 g) added to pot (1.5 kg soil) Myco-remediation efficiency was 85% from polluted soil, depending on the type of substrates and application method [90] Organic micro-pollutants, such as diclofenac and bicalutamide Substrate content was 200 g L −1 during 14 and 36 d…”

Fungal assisted bio‐treatment of environmental pollutants with comprehensive emphasis on noxious heavy metals: Recent updates

“…Jureczko and Przystaś (2021) performed a study in which they used white rot fungi for the removal of bleomycin and vincristine, two cytostatic drugs. Pleurotus ostreatus (BWPH), Trametes versicolor (CB8), Phyllotopsis nidulans (CB14), Hypholoma fasciculare (CB15), and Fomes fomentarius (CB13) were used in this study.…”

“…Pleurotus ostreatus (BWPH), Trametes versicolor (CB8), Phyllotopsis nidulans (CB14), Hypholoma fasciculare (CB15), and Fomes fomentarius (CB13) were used in this study. Their result showed the highest sorption of bleomycin by T. versicolor (CB8) while P. nidulans (CB14) was best for the sorption of vincristine (Jureczko & Przystaś, 2021). Dalecka et al (2020) have tested the capability of fungal isolates to grow in the presence of ibuprofen, diclofenac, sulfamethoxazole, and carbamazepine.…”

Bibliometric analysis of global research on white rot fungi biotechnology for environmental application

“…In recent studies, whole-cell WRF or its extracellular extracts have been successfully applied to remove trace organic contaminants (TrOC) in aqueous phase (Asif et al 2017a). Whole-cell WRF or their extracellular lignin-modifying enzymes (LMEs) have also been reported to degrade pesticide, graphene-related materials, pharmaceuticals, and personal care products that are persistent to the environment (Asif et al 2017b;Xiao and Kondo 2020b;Carniel et al 2021;Jureczko and Przystas 2021). Meanwhile, the use of cross-linked enzyme aggregates (CLEAs) such as porous-, combi-, or magnetic CLEAs to immobilize LMEs is a promising technology to overcome the stability and non-reusability of free LMEs from WRF in wastewater treatment (Vrsanska et al 2018;Voberkova et al 2018).…”