The study presents the removal of Cu(II) ions using bio‐adsorbents. The applicability of the continuous column process using hyacinth root, rice husk, blackberry leaves, and guava leaves is reported in this study. The effects of solution pH were studied in batch mode, and other different process parameters such as bed depth, rate of flow, influent solution concentration were investigated and optimized in the continuous mode. The breakthrough curves were obtained for various initial metal concentrations (10, 20, and 30 mg.L−1), flow rates (10, 30, and 40 ml.min−1), and bed height (4, 6, and 10 cm). The optimum operating conditions were 10 cm bed depth, 10 ml.min−1 flow rate, 10 mg.L−1 influent concentration, and pH 6. The breakthrough time was increased with increasing bed height and decreasing flow rate and influent concentration. The Freundlich isotherm model was in good agreement (R2 = .9247–.9987) with the batch experimental data whereas, the continuous experimental data fitted well in the Thomas model to illustrate the best performance of the fixed‐bed process. FTIR spectra and SEM images verify the sorption capacity of the adsorbents. The Cu(II) adsorption capacities of the adsorbents were compared, and the hyacinth root gives the best result compare to the others. Hence, the results proved the effectiveness of the adsorbents for the removal of Cu(II) ions.