Removal of tightly bound endotoxin from biological products

Wilson, Mark; Haggart, Claire L.; Gallagher, Seán P.; Walsh, Deirdre

doi:10.1016/s0168-1656(01)00256-5

Cited by 50 publications

(18 citation statements)

References 12 publications

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“…The resulting 200 ml of phage lysate was centrifuged in an Optima LE-80k ultracentrifuge (Beckman) for 70 min at 371,000 ϫ g, and the pellet was resuspended in 7 ml sterile Milli-Q water and filter sterilized using a sterile 0.45-m mixed cellulose ester Millex syringe filter unit (Millipore). Endotoxin was removed from the final phage preparation using either a Detoxi-Gel endotoxin removing column (Thermo Scientific) or a Pierce high-capacity endotoxin removal spin column (Thermo Scientific) (39,40). The heat-inactivated phage preparation was similar, but after endotoxin removal, the phage stock was incubated at 80°C for 15 min.…”

Section: Methodsmentioning

confidence: 99%

Aerosol Phage Therapy Efficacy in Burkholderia cepacia Complex Respiratory Infections

Semler

Goudie

Finlay

et al. 2014

Antimicrob Agents Chemother

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Phage therapy has been suggested as a potential treatment for highly antibiotic-resistant bacteria, such as the species of the Burkholderia cepacia complex (BCC). To address this hypothesis, experimental B. cenocepacia respiratory infections were established in mice using a nebulizer and a nose-only inhalation device. Following infection, the mice were treated with one of five B. cenocepacia-specific phages delivered as either an aerosol or intraperitoneal injection. The bacterial and phage titers within the lungs were assayed 2 days after treatment, and mice that received the aerosolized phage therapy demonstrated significant decreases in bacterial loads. Differences in phage activity were observed in vivo. Mice that received phage treatment by intraperitoneal injection did not demonstrate significantly reduced bacterial loads, although phage particles were isolated from their lung tissue. Based on these data, aerosol phage therapy appears to be an effective method for treating highly antibiotic-resistant bacterial respiratory infections, including those caused by BCC bacteria.

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Section: Methodsmentioning

confidence: 99%

Aerosol Phage Therapy Efficacy in Burkholderia cepacia Complex Respiratory Infections

Semler

Goudie

Finlay

et al. 2014

Antimicrob Agents Chemother

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“…Because Triton X-100 hinders hydrophobic interaction 56,57) , CPC adsorbed to polymers through hydrophobic interaction is desorbed by this surfactant. An NaCl solution is effective to assess the involvement of electrostatic interaction in the binding of CPC to polyHEMA/TMPT since CPC is cationic and shows strong interaction with negatively charged substances 27) .…”

Section: Loading Of Cetylpyridinium Chloride Using the Immersion Methodsmentioning

confidence: 99%

Non-biodegradable polymer particles for drug delivery: A new technology for “bio-active” restorative materials

Imazato

Kitagawa

Tsuboi

et al. 2017

Dental Materials Journal

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To develop dental restorative materials with "bio-active" functions, addition of the capability to release active agents is an effective approach. However, such functionality needs to be attained without compromising the basic properties of the restorative materials. We have developed novel non-biodegradable polymer particles for drug delivery, aimed for application in dental resins. The particles are made using 2-hydroxyethyl methacrylate (HEMA) and a cross-linking monomer trimethylolpropane trimethacrylate (TMPT), with a hydrophilic nature to adsorb proteins or water-soluble antimicrobials. The polyHEMA/TMPT particles work as a reservoir to release fibroblast growth factor-2 (FGF-2) or cetylpyridinium chloride (CPC) in an effective manner. Application of the polyHEMA/ TMPT particles loaded with FGF-2 to adhesives, or those loaded with CPC to resin-based endodontic sealers or denture bases/crowns is a promising approach to increase the success of the treatments by conferring "bio-active" properties to these materials to induce tissue regeneration or to inhibit bacterial infection.

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“…It is of note that although an endotoxin clearance factor of several fold can be achieved by various treatments, none of the methods described eliminate endotoxin entirely [65,66] and the residual concentration may be sufficient to induce significant cytokine secretion. Furthermore, a particular method of removal may work for one recombinant protein but not another [68,69]. The use of affinity columns to remove endotoxin from recombinant proteins is further complicated by the fact that the protein of interest is usually very tightly bound to endotoxin components contained within the preparation and removal of the endotoxin has the added danger that it also removes protein from the solution of interest [68].…”

Section: Decontaminating the Contaminantsmentioning

confidence: 99%