In this study, loaded Luffa sponge membrane forms were modified with ZnO, Fe 3 O 4 , ZnO/ Fe 3 O 4 nanoparticles (NPs) to remove of Direct Blue 15 (DB15), which is a carcinogenic azo dye in aqueous solution. ZnO and Fe 3 O 4 NPs were synthesized using purified peroxidase enzymes from Euphorbia amygdaloides using green synthesis method. Adsorption of DB15 azo dyes was separately studied with membrane forms (LS-pure, LS-ZnO, LS-Fe 3 O 4 , and LS-ZnO/Fe 3 O 4). Optimum contact time, optimum pH, optimum temperature, optimum dye concentration, and optimum LS amount were found as 45 min, pH 8.0, 20°C, 200 mg/L, and 0.025 g in line with the optimization studies, respectively. The obtained membrane forms were characterized using SEM, FT-IR, and XRD techniques. According to obtained results, NPs loaded LS membrane forms are promising in removal of DB15 from textile wastewater contaminated water.