Silver nanoparticles are used an increased attention for various biomedical and medical applications. In this study, green synthesis of silver nanoparticles was made with simple method by using peroxidase enzyme partially purified fromEuphorbia(Euphorbia amygdaloides) plant. Optimum pH, temperature and time period were determined to obtain silver nanoparticles using the peroxidase enzyme. The result shows that higher silver nanoparticle was synthesized for 4 hours and at 20°C and pH 8. Also, optimal concentration of metal ions was found as 0.5 mM. The synthesized silver nanoparticles were characterized by UV spectrum, scanning electron microscope (SEM) and X-ray diffraction. Antibacterial activity of silver nanoparticles was measured against some microorganisms such asSerratia marcescens, Yersinia pseudotuberculosis, Klebsiella pneumoniae, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Pseudomonas aeruginosa, Salmonella typhimurium, Listeria monocytogenes, and Escherichia coli. Synthesized silver nanoparticles have wide spectrum antibacterial activity in low concentration and may be a good alternative therapeutic approach in medicine and pharmaceutical fields in future.
Phytase enzyme was purified from the Pinar melkior (Lactarius piperatus) mushroom using ammonium sulfate precipitation and DEAEsephadex ion exchange chromatography techniques. First, the purified phytase enzyme was covalently bound to the surface of magnetite-CTS NPs in yield of 87%. Then, optimum pHs of the free and immobilized enzymes were determined as 5.0 and 4.0, respectively. Optimum temperature of free and immobilized enzymes was found as 60°C. Also, the effects of some metal ions on activity of free and immobilized phytase enzymes were investigated. Also, research was undertaken as to whether the purified free and immobilized phytase enzyme could hydrolyze the phytic acid in many cereal products or not. And, it was discovered that the immobilized phytase enzyme hydrolyzed the phytic acid at the highest rate (75.02% rate) in wheat. From the findings obtained, that immobilized enzyme was quite resistant to temperature, pH and metal ions.Se purificó la enzima fitasa de la seta Pinar melkior (Lactarius piperatus) utilizando precipitación de sulfato de amonio y técnicas cromatográficas de intercambio iónico DEAE con sephadex. Primeramente, la enzima fitasa purificada fue ligada de forma covalente a la superficie de CTS NP con magnetita con una eficiencia del 87%. Después, el pH óptimo de las enzimas libres e inmovilizadas fue determinado como 5,0 y 4,0 respectivamente. La temperatura óptima de las enzimas libres e inmovilizadas se encontró a 60°C. Además, se investigaron los efectos de algunos iones metálicos en la actividad de las enzimas fitasas libres e inmovilizadas. También, se estudió si las enzimas fitasas libres e inmovilizadas purificadas podrían hidrolizar el ácido fítico en varios productos que contengan cereales. Además, se determinó que las enzimas fitasa inmovilizadas, hidrolizaron el ácido fítico con el valor más alto (75,02%) en el trigo. A partir de estos resultados se encontró que las enzimas inmovilizadas eran muy resistentes a la temperatura, pH e iones metálicos.
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