Removal of pathogenic bacteria and nitrogens by Lactobacillus spp. JK-8 and JK-11

Ma, Chae‐Woo; Cho, Yun-Seok; Oh, Keun-Yeob

doi:10.1016/j.aquaculture.2008.10.061

Cited by 71 publications

(33 citation statements)

References 21 publications

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“…Synergistic removal of pathogenic bacteria with simultaneous decrease in ammonia, nitrite and nitrate level have already been reported in an in vitro assay using L. plantarum and L. hilgardii as candidate probiotic bacteria (Ma et al . ). However, in contrast to earlier findings, the present study showed inconsistent result where none of the water quality parameters improved significantly compared to the control.…”

Section: Discussionmentioning

confidence: 97%

Evaluation ofLactobacillus plantarumas a water additive on host associated microflora, growth, feed efficiency and immune response of giant freshwater prawn,Macrobrachium rosenbergii(de Man, 1879)

et al. 2014

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A 90‐day experiment was conducted by rearing 1020 prawn juveniles (0.54 ± 0.03 g) in water supplemented with three different concentrations of probiotic bacteria viz. T1 (107 cfu L−1), T2 (108 cfu L−1), T3 (109 cfu L−1) and the control (C) (unsupplemented water), to evaluate probiotic effect of Lactobacillus plantarum. In the present study, the growth parameters (WG%, SGR) and feed utilization parameters (FCR, PER) significantly improved (P < 0.05) in T3. The growth and feed utilization parameters though improved marginally in T1 and T2, the difference was not significant (P > 0.05) compared to the control. The gastro‐intestinal Lactobacillus sp. count increased significantly (P < 0.05) in all the treatment groups, whereas the decrease in harmful bacteria was significant (P < 0.05) in T3 compared to the control. Similarly, the Lactobacillus sp. count in culture water increased significantly (P < 0.05) in all the experimental groups, whereas the decrease in harmful bacteria was significant (P < 0.05) in T2 and T3. The immune parameters (THC, PO and RB activity) and clearance efficiency significantly improved (P < 0.05) in T3 with concurrent decrease (P < 0.05) in cumulative mortality against Aeromonas hydrophila challenge. However, water quality did not improved (P > 0.05) in any of the treatment groups. The results indicate that Lactobacillus plantarum at a minimum concentration of 109 cfu L−1 could be used as water additive to confer its probiotic effect in prawn, Macrobrachium rosenbergii. Moreover, future studies with higher probiotic concentrations should be conducted for its efficient commercial scale field application.

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Section: Discussionmentioning

confidence: 97%

Evaluation ofLactobacillus plantarumas a water additive on host associated microflora, growth, feed efficiency and immune response of giant freshwater prawn,Macrobrachium rosenbergii(de Man, 1879)

et al. 2014

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“…JK-8 and JK-11 had remarkable antimicrobial activities against target pathogens (e.g. Vibrio parahaemolyticus, Vibrio harveyi, and Edwardsiella tarda) [21] and removed the pathogens within 4 min of exposure [22]. Balcázar et al [4] report that the neutralised supernatants of Lactococcus lactis CLFP-101 can inhibit the growth of fish pathogens, A. hydrophila, A. salmonicida, Vibrio anguillarum, and Yersinia ruckeri.…”

Section: Discussionmentioning

confidence: 98%

Antagonistic Activity of Cellular Components of Potential Probiotic Bacteria, Isolated from the Gut of Labeo rohita, Against Aeromonas hydrophila

Giri

Sukumaran

Sen

et al. 2011

Probiotics & Antimicro. Prot.

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The objective of this study was to characterise the antagonistic activity of cellular components of potential probiotic bacteria isolated from the gut of healthy rohu (Labeo rohita), a tropical freshwater fish, against the fish pathogen, Aeromonas hydrophila. Three potential probiotic strains (referred to as R1, R2, and R5) were screened using a well diffusion, and their antagonistic activity against A. hydrophila was determined. Biochemical tests and 16S rRNA gene analysis confirmed that R1, R2, and R5 were Lactobacillus plantarum VSG3, Pseudomonas aeruginosa VSG2, and Bacillus subtilis VSG1, respectively. Four different fractions of cellular components (i.e. the whole-cell product, heat-killed whole-cell product [HKWCP], intracellular product [ICP], and extracellular product) of these selected strains were effective in an in vitro sensitivity test against 6 A. hydrophila strains. Among the cellular components, the ICP of R1, HKWCP of R2, and ICP of R5 exhibited the strongest antagonistic activities, as evidenced by their inhibition zones. The antimicrobial compounds from these selected cellular components were partially purified by thin-layer and high-performance liquid chromatography, and their properties were analysed. The ranges of pH stability of the purified compounds were wide (3.0-10.0), and compounds were thermally stable up to 90 °C. Considering these results, isolated probiotic strains may find potential applications in the prevention and treatment of aquatic aeromonosis.

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“…Bacteriocin is assumed to prevent bacteria development, especially pathogenic bacteria from overgrowing and has the ability to inhibit other bacteria and also able to stimulate the immune systems (Voravutikunchai et al, 2006;Aroutcheva et al, 2001). Application to the aquatic organism resulted in the increase of immune response against both gram-negative and gram-positive fish pathogens by the acid production (Ma et al, 2009;Aly et al, 2008).…”

Section: Resultsmentioning

confidence: 99%

THE EFFECT OF FEEDING Lactobacillus ON GROWTH, SURVIVAL RATE AND PROTEASE ACTIVITY OF Litopenaeus vannamei

Nafiqoh

Chang

Wang

2011

Indonesian Aquaculture Journal

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This study examined the effect of two Lactobacillus bacteria on protease activity and growth rate of Litopenaeus vannamei. An experiment was conducted to examine protease activity and growth rate. The experiment consisted of two treatment tanks, the first tank was provided with artemia immersed in 2.6 x 10 16 cfu/mL of bacteria solution, the second tank served as the control tank. After 20 days, the L. vannamei in the tank that received Lactobacillus have significantly different in growth, survival rate and protease activity (P<0.05) compared to the control, but no significant difference between Lactobacillus casei and Lactobacillus plantarum treatments. Within the digestive organ, protease activity of hepatopancreas and stomach demonstrated significant higher activity (P<0.05) compared to the intestine.

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Removal of pathogenic bacteria and nitrogens by Lactobacillus spp. JK-8 and JK-11

Cited by 71 publications

References 21 publications

Evaluation ofLactobacillus plantarumas a water additive on host associated microflora, growth, feed efficiency and immune response of giant freshwater prawn,Macrobrachium rosenbergii(de Man, 1879)

Evaluation ofLactobacillus plantarumas a water additive on host associated microflora, growth, feed efficiency and immune response of giant freshwater prawn,Macrobrachium rosenbergii(de Man, 1879)

Antagonistic Activity of Cellular Components of Potential Probiotic Bacteria, Isolated from the Gut of Labeo rohita, Against Aeromonas hydrophila

THE EFFECT OF FEEDING Lactobacillus ON GROWTH, SURVIVAL RATE AND PROTEASE ACTIVITY OF Litopenaeus vannamei

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