Removal of biofilms using carbon dioxide aerosols

Kang, Min-Yeong; Jeong, Hyun‐Woo; Kim, Jaeeun; Lee, Jinwon; Jang, Jaesung

doi:10.1016/j.jaerosci.2010.08.005

Cited by 19 publications

(16 citation statements)

References 16 publications

(22 reference statements)

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“…Table 1 lists numerous approaches (ultrasonication, electric fields, mild chemicals such as enzymes, and their combinations [11-17]) that have been employed and reported by other groups for this purpose. As can be seen, their efficacy is limited (they achieve only 1 to 3 log 10 reductions in the number of viable bacteria per unit area) and/or take a long time (hours).…”

Section: Introductionmentioning

confidence: 99%

Ultra-rapid elimination of biofilms via the combustion of a nanoenergetic coating

et al. 2013

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BackgroundBiofilms occur on a wide variety of surfaces including metals, ceramics, glass etc. and often leads to accumulation of large number of various microorganisms on the surfaces. This biofilm growth is highly undesirable in most cases as biofilms can cause degradation of the instruments and its performance along with contamination of the samples being processed in those systems. The current “offline” biofilm removal methods are effective but labor intensive and generates waste streams that are toxic to be directly disposed. We present here a novel process that uses nano-energetic materials to eliminate biofilms in < 1 second. The process involves spray-coating a thin layer of nano-energetic material on top of the biofilm, allowing it to dry, and igniting the dried coating to incinerate the biofilm.ResultsThe nanoenergetic material is a mixture of aluminum (Al) nanoparticles dispersed in a THV-220A (fluoropolymer oxidizer) matrix. Upon ignition, the Al nanoparticles react with THV-220A exothermically, producing high temperatures (>2500 K) for an extremely brief period (~100 ms) that destroys the biofilm underneath. However, since the total amount of heat produced is low (~0.1 kJ/cm2), the underlying surface remains undamaged. Surfaces with biofilms of Pseudomonas aeruginosa initially harboring ~ 107 CFU of bacteria /cm2 displayed final counts of less than 5 CFU/cm2 after being subjected to our process. The byproducts of the process consist only of washable carbonaceous residue and gases, making this process potentially inexpensive due to low toxic-waste disposal costs.ConclusionsThis novel method of biofilm removal is currently in the early stage of development. However, it has potential to be used in offline biofilm elimination as a rapid, easy and environmentally friendly method.

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Section: Introductionmentioning

confidence: 99%

Ultra-rapid elimination of biofilms via the combustion of a nanoenergetic coating

et al. 2013

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“…In a previous study by Kang et al (2010), CO 2 aerosols were shown to be an effective tool for removing biofilms from silicon chips, with up to 2-log reduction in the bacterial numbers after a treatment for 90 s. In addition, the aerosol treatment removed the EPS produced by the biofilm. However, the viability of the cells ejected from the surface was not evaluated and, if they remain viable, this poses a particular concern if this technique is to be used to remove biofilms composed of pathogenic bacteria from surfaces, such as medical equipment.…”

Section: Introductionmentioning

confidence: 89%

“…The general set-up ( Figure 1) and sequences of the CO 2 aerosol experiments were described in Kang et al (2010). Briefly, a dual gas unit (K6-10DG, Applied Surface Technologies, NJ) was used for aerosol generation and it incorporated an asymmetric Venturi nozzle whose orifice was *400 mm.…”

Section: Co 2 Aerosol Treatmentmentioning

confidence: 99%

Combined application of bacterial predation and carbon dioxide aerosols to effectively remove biofilms

et al. 2012

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“…Carbon dioxide (CO 2 ) snow jets are widely used in medical and industrial applications, such as cryogenic therapy , food processing, pharmaceutical granulation, particle removal, and surface cleaning (Yang et al 2007;Kang et al 2010;Liu et al 2011;Shen et al 2012). In semiconductor fabrication, CO 2 snow cleaning has unique eco-friendly organic and particle removal abilities that are better than those of traditional surface cleaning methods, such as ultra-sonic cleaning, mega-sonic cleaning, and brush scrubbing (Sherman et al 1991;Sherman 2007).…”

Section: Introductionmentioning

confidence: 99%

Agglomeration Processes and Mechanisms of CO₂Snow Inside a Tube

Lin

Shen

Wang

2013

Aerosol Science and Technology

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This article experimentally investigates the agglomeration mechanism of CO 2 primary particles inside a tube. The results show that a complicated particle motion in the upper portion of the tube is responsible for the formation of large snow particles. The high speed and complicated motion of the snow particles inside the tube provide both the opportunities and time for the collision of particles, which implies that only particle deposition and re-entrainment cannot completely describe the phenomenon of particle agglomeration. The results also show the mechanisms of particle agglomeration inside a tube, which include primary particle agglomerate in jet vortexes, agglomerated particles flowing upward into the recirculation region, particle clusters growing in the recirculation flow, and finally particles being released with the jet flow. A minimum tube length (30 mm in this case) is needed to ensure the complete formation of the agglomeration mechanisms with recirculation flow, and thus the formation of considerable amounts of agglomerated particles. The results of this study thus improve current understanding of the agglomeration process and mechanisms of CO 2 snow formation inside a tube.

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Removal of biofilms using carbon dioxide aerosols

Cited by 19 publications

References 16 publications

Ultra-rapid elimination of biofilms via the combustion of a nanoenergetic coating

Ultra-rapid elimination of biofilms via the combustion of a nanoenergetic coating

Combined application of bacterial predation and carbon dioxide aerosols to effectively remove biofilms

Agglomeration Processes and Mechanisms of CO₂Snow Inside a Tube

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Removal of biofilms using carbon dioxide aerosols

Cited by 19 publications

References 16 publications

Ultra-rapid elimination of biofilms via the combustion of a nanoenergetic coating

Ultra-rapid elimination of biofilms via the combustion of a nanoenergetic coating

Combined application of bacterial predation and carbon dioxide aerosols to effectively remove biofilms

Agglomeration Processes and Mechanisms of CO2Snow Inside a Tube

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Agglomeration Processes and Mechanisms of CO₂Snow Inside a Tube