Removal of bacterial and yeast contamination fromLeishmaniapromastigote cultures, by agar plating

Agar barrier method for removal of yeast and other fungal contamination fromLeishmaniapromastigotes culture

“…The plate was incubated at 2461uC for 15 days with regular microscopic examination. 5 Some distinct colonies (Pale-white, dome shaped and mucoid) were observed. A colony was picked and diluted in balanced salt solution for microscopic examination.…”

“…A few other physical as well as biological methods for the removal of yeast and other fungal contamination, had been explored such as rapid ion-exchange chromatography, 6 culture in sterilized U-tubes, 1 agar plating method. 5 These methods were, however, more cumbersome and time-consuming as well as less reproducible. The method described in the present study is novel and quite convenient for removal of fungal contamination from Leishmania culture at a single attempt in limited time to save important isolates.…”

“…Agar plating method 5 allows cloned promastigotes which may differ from original properties of archived sample. In connection to the above facts, an inexpensive, easy to perform, rapid and reliable, physical method has been developed to remove yeast and other fungal contamination, which is able to save valuable isolates in culture.…”

Evaluation of the Reproduction on Blood and Chocolate Agars of Leishmania Isolates Obtained from Turkey

“…Sık pasaj yapılması kültürlerin bakteri veya mantarlarla kontamine olma riskini arttırmaktadır. Aynı zamanda biyolojik özelliklerinin değişmesine, virülanslarının azalmasına ve antijenik yapılarını kaybetmelerine neden olmaktadır (4) . Liyofilizasyon ya da kriyoprezervasyon yoluyla izolatların uzun süre korunması olasıdır.…”

Long-term preservation ofLeishmania donovanipromastigotes on blood-agar slants

“…This method, which is based on the maintenance of the parasites on blood-agar slants, with no overlay, in a refrigerator, has been developed and evaluated at the Rajendra Memorial Research Institute of Medical Sciences (RMRIMS), in Patna, India. For the evaluation, splenic aspirates from three cases of visceral leishmaniasis who had been admitted to the inpatient ward at the RMRIMS were cultured on NNN medium (Muniaraj et al, 2005). After primary isolation, which was successful for all three cases, the promastigotes were transferred to tubes of fresh NNN medium overlain with Locke's solution, and incubated at 24¡1uC for 1 week (i.e.…”