Removal of antibiotic residues, antibiotic resistant bacteria and antibiotic resistance genes in municipal wastewater by membrane bioreactor systems

Le, Thai-Hoang; Ng, Charmaine; Trần, Ngọc Hân; Chen, Hongjie; Gin, Karina Yew-Hoong

doi:10.1016/j.watres.2018.08.060

Cited by 278 publications

(112 citation statements)

References 46 publications

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“…Although low in 73 carbon, AnMBR effluents are rich in nutrients and can be utilized effectively in agricultural 74 reuse scenarios to offset artificial fertilizer needs. Further, the combination of membrane 75 separation with slower microbial growth under anaerobic conditions can theoretically 76 reduce the load of ARGs and ARB in treated wastewater (Le, et al, 2018). However, the 77 fate of ARGs and ARB within AnMBRs remains poorly understood.…”

Section: Introduction 44mentioning

confidence: 99%

Comparative analysis of intracellular and extracellular antibiotic resistance gene abundance in anaerobic membrane bioreactor effluent

Wang

Harb

Zarei-Baygi

et al. 2019

Preprint

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The growing practice of wastewater reuse poses a significant risk to further 13 dissemination of antibiotic resistance due to the abundance of antibiotic resistance 14 bacteria (ARB) and antibiotic resistance genes (ARGs) in wastewater effluents. Anaerobic 15 membrane bioreactors (AnMBRs) are an emerging wastewater treatment technology 16 capable of reducing the total ARGs and ARB load discharged to receiving environments 17 compared to conventional aerobic treatment processes. While size exclusion is effective 18 at retaining ARB and its associated intracellular ARGs, the abundance and fate of 19 extracellular ARGs in an AnMBR effluent have not been examined. This study elucidates 20 the effect of combined antibiotics loading (ampicillin, erythromycin, and 21 sulfamethoxazole) on the abundance of intracellular and extracellular ARGs in an AnMBR 22 chemical oxygen demand (sCOD), total chemical oxygen demand (tCOD), volatile fatty 128 acids (VFAs), and mixed liquor and volatile suspended solids (MLSS/MLVSS). Biogas 129 production was monitored by an in-line gas flowmeter and assessed for methane content 130 by gas chromatography with flame ionization detection (GC-FID). Steady AnMBR

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Section: Introduction 44mentioning

confidence: 99%

Comparative analysis of intracellular and extracellular antibiotic resistance gene abundance in anaerobic membrane bioreactor effluent

Wang

Harb

Zarei-Baygi

et al. 2019

Preprint

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“…The gene bla CTX‐M was not detected in the influents of both processes in spring and winter. Other researchers have also reported that MBRs effectively reduce ARGs in effluents up to 7 log reductions (Le et al, ; Li, Qiu, et al, ; Li, Jay, et al, ). However, apart from the gene cmlA rest of the ARGs was still detected up to 10 4 copy number/ml in the effluents of the MBR and package MBR processes tested in this study.…”

Section: Resultsmentioning

confidence: 92%

“…Removal efficiencies of ARGs and 16S rDNA gene for each WWTP were measured with log removal value (LRV) (Le et al, ). Log removal values were determined by taking logarithm of the ratio of gene concentrations in the influents and effluents of the WWTPs as shown in Equation ():

LRV = {Log}_{10} (C_{influent} / C_{effluent})

…”

Section: Methodsmentioning

confidence: 99%

“…Antibiotic resistance genes are typically carried on highly transmissible genetic materials like plasmids, integrons, and transposons (van Hoek et al, ) that are commonly present in bacteria‐rich areas like water resources recovery facilities (WWTPs) (Furuya & Lowy, ). Water resources recovery facilities also receive discharges from hospitals, residential, and agricultural areas, and these discharges contain antibiotic residues, antibiotic resistant bacteria and ARGs (Le, Ng, Tran, Chen, & Gin, ; Tran et al, ). Therefore, WWTPs create favorable conditions for the HGT of ARGs and contribute to the dissemination of antibiotic resistance further (Schlüter et al, ).…”

Section: Introductionmentioning

confidence: 99%

“…Although WWTPs are designed for the removal of organic substrates and nutrients, their efficiency for the removal of ARGs is still incomplete and requires further consideration. Limited studies have been conducted on full‐scale WWTPs on the removal of ARGs (Le et al, ; Mao et al, ). Studies about biological nutrient removal (BNR) (Mao et al, ) and conventional activated sludge (CAS) (Yang, Li, Zou, Fang, & Zhang, ) showed that BNR and CAS systems partially remove ARGs with 0–3 log reductions while sequencing batch reactor (SBR) poorly removes ARGs with 0–1 log reductions (Korzeniewska & Harnisz, ).…”

Section: Introductionmentioning

confidence: 99%

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Removal of antibiotic resistance genes in various water resources recovery facilities

Kucukunsal

İçgen

2020

Water Environment Research

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Water resources recovery facilities (WWTPs) are hotspots for antibiotic resistance genes (ARGs) and pose a significant threat to environments. Therefore, ARG removal efficiencies of WWTPs are of great importance. In this study, conventional activated sludge (CAS), biological nutrient removal (BNR), sequencing batch reactor (SBR), membrane bioreactor (MBR), package MBR, and WWTP with coagulation-flocculation and UV disinfection units were investigated in terms of their removal efficiencies on overall bacterial genes with 16S rDNA and seven ARGs including the genes aadA, bla CTX-M , cmlA, ermB, sul1, tetA, and qnrS corresponding to commonly used antibiotics aminoglycosides, β-lactams, amphenicols, macrolides-lincosamides-streptogramins, sulfonamides-trimethoprim, tetracyclines, and quinolones, respectively. Seasonal abundance of overall genes and ARGs in influents and effluents of each WWTPs was determined by quantitative polymerase chain reaction. Membrane bioreactor and package MBR systems showed the highest removal efficiency up to 5-log reductions. Seasonal changes affected ARG removal efficiencies of BNR and CAS significantly (p < 0.05). The lowest log reductions were determined in summer for BNR and in both autumn and winter for CAS. The abundance of the genes increased in sludge treatment of CAS. In all WWTPs tested, ARG removal rates were correlated with the 16S rDNA gene removal (p > 0.05). The results elucidated that the removal mechanism was not ARG-specific. © 2019 Water Environment Federation • Practitioner points• Removal of genes in WWTPs was found to be not ARG-specific. • MBR and package MBR systems had the highest gene removal efficiencies. • Effluents of the MBR systems still retained up to 10 4 gene copy number/ml. • Removal of genes in CAS and BNR systems showed seasonal variations. • The copy numbers of the genes increased in the sludge of CAS.•

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Aerobic Biological Treatment of Microconstituents

Chen

Nguyen

Chen

et al. 2023

Microconstituents in the Environment

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Removal of antibiotic residues, antibiotic resistant bacteria and antibiotic resistance genes in municipal wastewater by membrane bioreactor systems

Cited by 278 publications

References 46 publications

Comparative analysis of intracellular and extracellular antibiotic resistance gene abundance in anaerobic membrane bioreactor effluent

Comparative analysis of intracellular and extracellular antibiotic resistance gene abundance in anaerobic membrane bioreactor effluent

Removal of antibiotic resistance genes in various water resources recovery facilities

Aerobic Biological Treatment of Microconstituents

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