Comparative analysis of intracellular and extracellular antibiotic resistance gene abundance in anaerobic membrane bioreactor effluent

Wang, Phillip; Harb, Moustapha; Zarei-Baygi, Ali; Stadler, Lauren B.; Smith, Adam L.

doi:10.1101/702076

Cited by 4 publications

(6 citation statements)

References 51 publications

(16 reference statements)

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“…Other sulfa-ARGs showed a lower abundance in the R 1 effluent, while sul1 and sul2 were highly expressed in R 2 (Figure S9), consistent with Zhang et al’s report, in which long-term removal of SMX in the BGS system fostered the enrichment of sul1 and sul2 . Various-sized plasmids can act as carriers of these genes . Different trends were observed for other subtypes, with overall concentrations being significantly lower ( p < 0.05) in R 1 compared to R 2 (Figures S9–S12).…”

Section: Resultssupporting

confidence: 88%

“…Calero-Cáceres et al noted a transition in ARG prevalence during secondary treatment, with specific ARG types becoming more pronounced in sludge samples . Wang et al observed a striking effluent–biomass exchange of ARGs in the sludge reactor, which was plasmid-mediated . Here, the transposons and plasmids, representing >75% of the total MGEs (Figure S5), showed biomass preference at low antibiotic spiking, suggesting the biomass as an important source to potential HGT exchanges.…”

Section: Resultsmentioning

confidence: 88%

“…6 Various-sized plasmids can act as carriers of these genes. 45 Different trends were observed for other subtypes, with overall concentrations being significantly lower (p < 0.05) in R 1 compared to R 2 (Figures S9−S12). Notably, ARG subtypes in biomass and effluent showed somewhat similar occurrences, indicating the potential of ARG transition and exchanges between biomass and effluent due to system buoyancy and hydrodynamics.…”

Section: Characteristics and Performance Study 311 System Physical St...mentioning

confidence: 85%

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Unraveling the Role of Microalgae in Mitigating Antibiotics and Antibiotic Resistance Genes in Photogranules Treating Antibiotic Wastewater

Kiki,

Qin,

Liu

et al. 2023

Environ. Sci. Technol.

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Harnessing the potential of specific antibiotic-degrading microalgal strains to optimize microalgal-bacterial granular sludge (MBGS) technology for sustainable antibiotic wastewater treatment and antibiotic resistance genes (ARGs) mitigation is currently limited. This article examined the performance of bacterial granular sludge (BGS) and MBGS (of Haematococcus pluvialis, an antibiotic-degrading microalga) systems in terms of stability, nutrient and antibiotic removal, and fate of ARGs and mobile genetic elements (MGEs) under multiclass antibiotic loads. The systems exhibited excellent performance under none and 50 μg/L mixed antibiotics and a decrease in performance at a higher concentration. The MBGS showed superior potential, higher nutrient removal, 53.9 mg/L/day higher chemical oxygen demand (COD) removal, and 5.2−8.2% improved antibiotic removal, notably for refractory antibiotics, and the system removal capacity was predicted. Metagenomic analysis revealed lower levels of ARGs and MGEs in effluent and biomass of MBGS compared to the BGS bioreactor. Particle association niche and projection pursuit regression models indicated that microalgae in MBGS may limit gene transfers among biomass and effluent, impeding ARG dissemination. Moreover, a discrepancy was found in the bacterial antibiotic-degrading biomarkers of BGS and MBGS systems due to the microalgal effect on the microcommunity. Altogether, these findings deepened our understanding of the microalgae's value in the MBGS system for antibiotic remediation and ARG propagation control.

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Section: Resultssupporting

confidence: 88%

Section: Resultsmentioning

confidence: 88%

Section: Characteristics and Performance Study 311 System Physical St...mentioning

confidence: 85%

See 1 more Smart Citation

Unraveling the Role of Microalgae in Mitigating Antibiotics and Antibiotic Resistance Genes in Photogranules Treating Antibiotic Wastewater

Kiki,

Qin,

Liu

et al. 2023

Environ. Sci. Technol.

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“…Finally, we calculated the removal of each target gene across the treatment processes during the 24 h sampling period based on gene loads, which accounted for the plant flow rate (Figure ). The load of ARGs in the treated effluent may be more informative as compared to the concentrations of ARGs to indicate ARG dissemination into effluent-receiving environments because it represents the actual abundance of ARGs released from a WWTP over a specified period of time. − We found that all target genes were reduced in terms of 24 h loads (Figure a,b), but the degree of removal varied for different targets and across different unit processes (Figure c,d).…”

Section: Resultsmentioning

confidence: 92%

Snapshot ARG Removal Rates across Wastewater Treatment Plants Are Not Representative Due to Diurnal Variations

Lou

Ali

et al. 2022

ACS EST Water

Self Cite

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To evaluate the threat of the environmental dissemination of antibiotic resistance associated with wastewater treatment plants (WWTPs), the removal efficiency of antibiotic resistance genes (ARGs) during wastewater treatment needs to be assessed. The sample collection strategy is one factor that is often overlooked in study design and most studies on ARGs in wastewater perform grab sampling. Here, we hypothesized that wastewater sampling (i.e., grab and composite sampling) influences the observed ARG concentrations and calculated removal rates across WWTPs. We compared the removal rates calculated based on the two different sampling methods for several genes, including some clinically relevant ARGs (blaNDM-1, blaOXA-1, MCR-1, MCR-5, MCR-10, and qnrA). We conducted summer and winter 24 h sampling campaigns where grab samples were collected every 2 h from the influent, secondary effluent, and final effluent. The snapshot removal rate of each target gene calculated based on the 12 grab samples fluctuated by 0.5–1.6 log in the winter and 0.9–2.7 log in the summer, indicating diurnal variation. Overall, for each target gene, the removal rates calculated based on 24 h composite samples were approximately equal to the median of the 12 snapshot removal rates. Our study confirms the importance of using composite samples to monitor ARGs in wastewater.

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“…For the ARGs, we noticed that tet-associated iARGs in the effluent increased and were affected by membrane pore size. This accentuates the conclusion drawn by Wang et al (2019) on the ramification of antibiotic exposure on the abundance of intracellular ARGs. The UF membrane demonstrated superior restraint of tet-asociated iARGs into the effluent in comparison to the MF1 membrane.…”

Section: Tetracycline Args In Effluents Before Vs After Tetracycline ...mentioning

confidence: 56%

Investigating the Fate of Tetracycline in an Anaerobic Membrane Bioreactor and Its Effects on Antibiotic Resistance Gene Proliferation from Different Membrane Types. (c2022)

Zeeb¹

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As the concern of fresh and clean water scarcity is raised worldwide, the necessity of water reuse stands out. In this sense, the AnMBR, an advanced wastewater treatment technology combining the advantages of the MBR and anaerobic processes, has gained much attention in the past years as it produces high quality effluent from an energy efficient mechanism. Even though the removal of emerging microbial contaminants generally and antibiotics specifically from wastewater has been studied, the reduction of ARGs and tetracycline, which is one of the most used antibiotics worldwide, hasn’t been explicitly looked into using AnMBRs during treatment of real municipal wastewaters. Hence, this research, composed of a pre-tetracycline addition phase and a tetracycline addition phase, aimed to examine the effect of tetracycline continuously fed at a concentration of 300 μg/L on the performance of a lab-scale AnMBR as well as to evaluate the removal efficiency of tetracycline via the AnMBR system while looking into the attribution of each removal mechanism. It also studied the proliferation of iARGs and eARGs in the effluents of three externally connected membranes: two MFs and one UF. The results showed that a slight and quickly recoverable disruption was imposed by tetracycline on the system. Tetracycline was reduced at an efficiency > 91% for the three effluents with adsorption to sludge being the primary mechanism of removal even though notable degradation averaged to 38 % was recorded. The contribution of adsorption of tetracycline to the biofilm layer developed on each of the three membranes to removal was negligible. For ARGs, an increase in the abundance of tet-associated iARGs, with variation depending on the membrane pore size, was detected in the effluent, in opposition to tet-associated eARGs and non-tet ARGs which did not show an interpretable response to tetracycline.

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Comparative analysis of intracellular and extracellular antibiotic resistance gene abundance in anaerobic membrane bioreactor effluent

Cited by 4 publications

References 51 publications

Unraveling the Role of Microalgae in Mitigating Antibiotics and Antibiotic Resistance Genes in Photogranules Treating Antibiotic Wastewater

Unraveling the Role of Microalgae in Mitigating Antibiotics and Antibiotic Resistance Genes in Photogranules Treating Antibiotic Wastewater

Snapshot ARG Removal Rates across Wastewater Treatment Plants Are Not Representative Due to Diurnal Variations

Investigating the Fate of Tetracycline in an Anaerobic Membrane Bioreactor and Its Effects on Antibiotic Resistance Gene Proliferation from Different Membrane Types. (c2022)

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