The systematic studies were carried out to determine the optimum process parameters, such as the hydrogen peroxide (H 2 O 2 ) concentration, concentration and molar mass of poly(ethylene glycol) (PEG) as an additive, pH value, temperature, and enzyme dose for treatment of bisphenol A (BPA) with soybean peroxidase (SBP). The SBP-catalyzed treatment of BPA was effectively enhanced by adding PEG in the presence of H 2 O 2 . BPA was completely treated by SBP of 0.50 U/cm 3 and water-insoluble oligomers were generated. The optimum conditions for SBP-catalyzed treatment of BPA at 0.3 mM were determined to be 0.3 mM of H 2 O 2 and 0.10 mg/cm 3 of PEG with molar mass of 1.0 3 10 4 g/mol in a pH 7.0 buffer at 308C. A variety of bisphenol derivatives were completely or effectively treated by SBP under the optimum conditions determined for treatment of BPA, although the SBP dose was further increased as necessary for some of them. The aggregation of water-insoluble oligomers generated was enhanced by decreasing the pH values of the solutions to 4.0 with HCl after the enzymatic treatment, and BPA and bisphenol derivatives were removed from aqueous solutions by filtering off the oligomer precipitates.