Removal characteristics of endocrine-disrupting chemicals by laccase from white-rot fungi

Abstract

Laccase from 5 white-rot fungal strains (4 Trametes and 1 Pycnoporus strains) were evaluated in the removal spectra with/without mediators against 11 EDCs. Purified laccase from Trametes sp. was also used to reveal the precise degradation spectra and degradation profiles in time course against 20 EDCs with/without mediators. In addition, effectivity of laccase for the purification of complex EDCs contamination was evaluated combining several EDCs. The removal characteristics among tested strains were almost the same and crude/purified laccase could remove various EDCs. Bis(4-hydroxyphenyl)sulfone, diethylhexylphthalate (DEHP), pyrene (PY), anthracene, 3,5-dichlorophenol and pentachlorophenol could not be removed by laccase. DEHP and PY could not be removed even with mediators. Vanillin and vanillic acid revealed to be possible naturally occurring mediators. Laccase-mediator system could expand the degradation spectrum and enhance the EDCs removal ratio and rate. When complex mixtures of EDCs were treated with laccase, the removal ratio was enhanced in comparison to that of single application. Some coexisting EDCs could act as mediators. Thus, the availability of laccase and the effectivity of a mediator on EDCs treatment were indicated.

Removal of estrogenic activity of iso-butylparaben and n-butylparaben by laccase in the presence of 1-hydroxybenzotriazole

“…3). These results suggest that degradation of parabens by laccase itself is possible, but is not sufficiently effective for application to the bioremediation of parabens, although several studies on laccase treatment of endocrine-disrupting (estrogenic) chemicals have demonstrated its efficiency (Tamagawa et al 2005(Tamagawa et al , 2006(Tamagawa et al , 2007Kim et al 2008;Sei et al 2008).…”

Degradation of selected pharmaceutical and personal care products (PPCPs) by white-rot fungi

“…This study demonstrated that after 7 days of incubation, ibuprofen was degraded by all four strains, while carbamazepine and clofibric acid were much more recalcitrant; only T. versicolor attained significant degradation of both of these compounds. The use of oxidative enzymes to oxidize PPCPs in vitro has been demonstrated to remove the estrogenic activities from genistein, bisphenol A, nonylphenol, estrone (E 1 ), 17b-estradiol (E 2 ), estriol (E 3 ) and ethinyl estradiol (EE 2 ) (Cabana et al 2007;Hirano et al 2000;Sei et al 2008;Soo-Min et al 2005;Tamagawa et al 2005Tamagawa et al , 2006Tsutsumi et al 2001).…”

Elimination and detoxification of triclosan by manganese peroxidase from white rot fungus

“…There is currently great interest in lignin-degrading white rot fungi and the ability of their ligninolytic enzymes, such as manganese peroxidase (MnP), lignin peroxidase (LiP) and laccase, to degrade endocrine disruptors and pharmaceuticals [8][9][10][11][12][13][14][15][16][17]. To date, several studies on the elimination of TCS using white rot fungus and/or laccase have been carried out.…”