Removal and biodegradation of polycyclic aromatic hydrocarbons by <i>Selenastrum capricornutum</i>

Chan, Siu-Ming; Luan, Tiangang; Wong, Ming Hung; Tam, N.F.Y.

doi:10.1897/05-354r.1

Cited by 150 publications

(72 citation statements)

References 35 publications

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“…Biosorption is a physic-chemical process involved in the sorption of a chemical substance in/on a biological matrix/ surface [16,17]. So far, the biosorption of heavy metal, dyes, and pesticides by biological materials such as bacteria, fungus and algae were extensively reported [1620], but the biosorption of POPs has received considerably less attention.…”

mentioning

confidence: 99%

Biosorption and biodegradation of polycyclic aromatic hydrocarbons by Phanerochaete chrysosporium in aqueous solution

2012

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Biosorption and biodegradation of phenanthrene and pyrene by live and heat-killed Phanerochaete chrysosporium are investigated to elucidate the bio-dissipation mechanisms of polycyclic aromatic hydrocarbons (PAHs) in aqueous solution and its regulating factors. The effects of nutrient conditions (carbon source and nitrogen source concentrations), the co-existing Cu 2+ , and repeated-batch feed of PAHs on the biosorption and biodegradation are systematically studied. The removal of PAHs by dead bodies of P. chrysosporium is attributed to biosorption only, and the respective partition coefficients of phenanthrene and pyrene are 4040 and 17500 L/kg. Both biosorption and biodegradation contribute to the dissipation of PAHs by live P. chrysosporium in water. After a 3-d incubation, the removal percentage via biosorption are 19.71% and 52.21% for phenanthrene and pyrene, respectively. With the increase of the incubation time (340 d), biodegradation gradually increases from 20.40% to 60.62% for phenanthrene, and from 15.55% to 49.21% for pyrene. Correspondingly, the stored-PAHs in the fungal bodies decrease. Under the carbon-rich and nitrogen-limit nutrient conditions, the removal efficiency and biodegradation of phenanthrene and pyrene are significantly promoted, i.e. 99.55% and 92.77% for phenanthrene, and 99.47% and 83.97% for pyrene after a 60-d incubation. This phenomenon is ascribed to enhanced-biosorption due to the increase of fungal biomass under carbon-rich condition, and to stimulated-biodegradation under nitrogen-limit condition. For the repeated-batch feed of phenanthrene, the pollutant is continuously removed by live P. chrysosporium, and the contribution of biodegradation is enhanced with the repeated cycles. After 3 cycles, the biodegradation percentage is up to 90% with each cycle of a 6-d incubation.biosorption, biodegradation, Phanerochaete chrysosporium, polycyclic aromatic hydrocarbons, bioremediation Citation:Ding J, Chen B L, Zhu L Z. Biosorption and biodegradation of polycyclic aromatic hydrocarbons by Phanerochaete chrysosporium in aqueous solution.

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confidence: 99%

Biosorption and biodegradation of polycyclic aromatic hydrocarbons by Phanerochaete chrysosporium in aqueous solution

2012

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“…The molecular weight, water solubility and lipophilicity of the compound would affect the bioaccumulation and degradation by microorganisms. The 4-ring PAHs, Xuoranthene and pyrene, were easier to remove and degrade by Selenastrum capricornutum than phenanthrene, a 3-ring PAH (Chan et al 2013). Similar to microalgae, Potinet et al (2004) reported that two filamentous fungi isolated from PAH-contaminated soil, namely Coniothyrium sp.…”

Section: Discussionmentioning

confidence: 99%

Some Intermediate Bio-Transformants During Biodegradation of High Molecular Weight Phenanthrene and Fluoranthene by Cyanobacterial species – Aulosira Fertilissima Ghose

Kumar¹,

Barot²,

Khan³

2013

Int J Appl Sci Biotechnol

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The PAHs compounds are known to be carcinogenic, teratogenic, mutagenic and toxic to all living organism. Handful of literature is available on biodegradation of these compounds by bacteria and fungi, however, scanty work is done by using microalgae on biodegradation of these two PAHs. In this investigation, the efficiency of Aulosira fertilissima Ghose to remove fluoranthene (0.001gm.ml -1 ), phenanthrene (0.001gm.ml -1 ) and a mixture of both (each at concentration of 0.0005gm.ml -1 ) were evaluated for intermediate bio-transformants during biodegradation by using GCMS. The result showed that the efficiency of Aulosira fertilissima for removal and biodegradation of phenanthrene was higher than fluoranthene, indicate fluoranthene was more stable and recalcitrant. PAHs uptake after 7-days of treatment was 80% and 66% of these phenanthrene and fluoranthene ,respectively by the cyanobacteria .The synergetic effect of fluoranthene on phenanthrene was observed, presence of fluoranthene stimulate the degradation of phenanthrene due to which phenanthrene produce more bio-transformants . Some intermediates were observed like Methyl linoleate, 4-(2,2-dimethyl-6-methylenecyclohexylidene)-3-methyl-,(Z)-etc. for phenanthrene biodegradation process while 2,3-dihydrofluoranthene, (1R,5R)-2-isopropyl-5-methylcyclohexanol, for fluoranthene degradation. Moreover, 3-isopropylidene-2,2-dimethyl-6-phenyl-1,4-oxathiane, 7-phenyltridecane, diphenylacetylene, for mixture of two PAHs applied.

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“…Cells cultured in modified SE medium under axenic condition were harvested after 10-12 days [15]. The algal suspension was mixed with heat-dissolved sodium alginate to form 3% algal alginate mixture, dropped into 2.5% calcium chloride solution by a peristaltic pump (Cole-Parmer Instrumental Company Masterflex L/S model 7519-06) to produce immobilized algal beads, each with a diameter of 3 mm.…”

Section: Preparation Of Immobilized Beadsmentioning

confidence: 99%

“…The dissolved alginate matrix was separated from the cells by centrifugation at 2,400 g for 15 min. The PAH concentrations in the medium, in alginate matrix and taken up by cells were extracted by ethyl acetate following the standard method [15], and determined by a Hewlett Packard 5890 gas chromatography (GC) equipped with a flame ionization detector (FID). The fused capillary column (J & W Scientific HP-5MS) was 30 m long with 0.25 mm internal diameter and was coated with methyl silicone of 0.25 µm film thickness.…”

Section: Effects Of Initial Cell Densities Per Bead On Pah Removalmentioning

confidence: 99%

Removal and biodegradation of polycyclic aromatic hydrocarbons by immobilized microalgal beads

Tam

Chan

Wong

2010

WIT Transactions on Ecology and the Environment

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Polycyclic aromatic hydrocarbons (PAHs) are a class of toxic, carcinogenic and/or mutagenic organic compounds, which must be removed from contaminated environments such as wastewater. The present study aims to explore the potential of a fresh water green microalga, Selenastrum capricornutum, immobilized in alginate beads to remove mixed PAHs and determine the optimal density for an effective removal. Results demonstrated that the immobilized microalgal beads were effective in removing and biodegrading mixed PAHs at their dissolved concentrations, namely phenanthrene (PHE, 1 mg l -1 ), fluoranthene (FLA, 0.25 mg l -1 ) and pyrene (PYR, 0.1 mg l -1 ). These abilities were significantly affected by cell density, ranging from 2.5x10 5 to 5x10 6 cells bead -1 . At the optimal cell density of 2.5x10 6 cells bead -1 , 92% PHE and all FLA were removed in 168 hours, and it only took 24 hours to completely remove PYR. In 168-hour treatment, 73% PHE was biodegraded while FLA and PYR were completely degraded. The removal and biodegradation of PAHs were further enhanced by increasing the number of beads used and the optimal bead density was 20 beads ml -1 wastewater. These findings suggested that PAHs were quickly adsorbed onto cells and the alginate matrix, absorbed within cells and further degraded by live cells immobilized in alginate beads.

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Removal and biodegradation of polycyclic aromatic hydrocarbons by Selenastrum capricornutum

Cited by 150 publications

References 35 publications

Biosorption and biodegradation of polycyclic aromatic hydrocarbons by Phanerochaete chrysosporium in aqueous solution

Biosorption and biodegradation of polycyclic aromatic hydrocarbons by Phanerochaete chrysosporium in aqueous solution

Some Intermediate Bio-Transformants During Biodegradation of High Molecular Weight Phenanthrene and Fluoranthene by Cyanobacterial species – Aulosira Fertilissima Ghose

Removal and biodegradation of polycyclic aromatic hydrocarbons by immobilized microalgal beads

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