Biosorption and biodegradation of polycyclic aromatic hydrocarbons by Phanerochaete chrysosporium in aqueous solution

Ding, Jie; Chen, Baoliang; Zhu, Lizhong

doi:10.1007/s11434-012-5411-9

Cited by 46 publications

(25 citation statements)

References 33 publications

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“…The adsorption of n -hexadecane by heat-killed microbial strain was a fast process, however, with the time increasing, the adsorbed n -hexadecane were released from dead bacterial strain. This phenomena was also obtained from the bioadsorption behavior of microbial cell for PAHs and alkane which was controlled by the distributional effects with the process of sorption-desorption being reversible133031. Rosenberg and Rosenberg32 had shown that the rate of hydrocarbon degradation by bacterial cells was dependent on cell affinity.…”

Section: Resultsmentioning

confidence: 93%

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Metabolic pathway for a new strain Pseudomonas synxantha LSH-7′: from chemotaxis to uptake of n-hexadecane

Long

Bao

et al. 2017

Sci Rep

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Bacteria can use n-hexadecane as a carbon source, but it remains incompletely understood whether n-hexadecane is transformed into metabolic intermediates prior to cellular uptake or not. We newly isolated a strain identified as Pseudomonas synxantha LSH-7′ and conducted chemotaxis experiment of this bacterial strain towards n-hexadecane, hexadecanol and hexadecanoic acid with qualitative assays respectively. Furthermore, we described the identification of extracellular alkane hydroxylase and alcohol dehydrogenase activity; acidification of the culture medium; identification of hexadecanoic acid in the culture medium by the GC-MS analysis; and variation concentration of intracellular n-hexadecane and hexadecanoic acid. A detailed analysis of the experimental data revealed the chemotaxis of this bacterial strain towards n-hexadecane instead of its metabolic intermediates. Our results further suggested that only a fraction of total n-hexadecane followed this path, and alkane hydrolase and hexadecanol dehydrogenase were constitutively expressed when grown in the medium of n-hexadecane. Most strikingly, we quantitatively investigated the concentration of n-hexadecane adsorbed by bacterial chemotaxis. Our findings provided an original insight n-hexadecane might be converted to hexadecanoic acid extracellularly before it was taken up across the cell membrane.Alkanes are found highly abundant constituents of petroleum hydrocarbons are major environmental pollutants as a result of terrestrial and freshwater runoff, refuse from coastal oil refineries, off shore oil production, shipping activities and accidental spills 1,2 . Among the alkanes, n-hexadecane is a major component. The solubility of n-hexadecane in water is 5.21 × 10 −5 mg L −1 at 15 °C and has high partitioning co-efficient 9.1 logKow 3 . Biodegradation of n-hexadecane is believed to be a friendly way to environment as well as human beings. For this, it is necessary to know the metabolic mechanisms of n-hexadecane.Singer and Finnerty 4 observed terminal n-hexadecane degradation by Acinetobacter HO1-N and Pseudomonas putida, and identified five intermediates as n-hexadecylhydroperoxide, n-hexadecanol, n-hexadecyldehyde, n-hexadecanoic acid and n-hexadecylhexadecanoate in degradation process. However, Whyte et al. 5 reported both terminal and sub-terminal oxidation of n-C16 by Rhodococcus strain Q15 producing 1-hexadecanol and 2-hexadecanol. Recently, a new insights into alkane metabolism pathways from alkane sensing, chemotaxis, signal transduction, uptake to degradation was put forward by Wang and shao 6 from the aspect of genes. The underlying mechanisms that control the early responses of bacterial cells to n-hexadecane, such as chemotaxis and uptake, remain largely unexplored.Chemotaxis is defined as the capacity of certain organisms to sense substance concentration gradients and in turn moved towards or away from this gradient 7-9 . Most importantly, alkane chemotaxis had been observed in bacterial isolates of Flavimonas oryzihabitans genus 10 , and even ...

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Section: Resultsmentioning

confidence: 93%

“…The concentrations of n -hexadecane that washed solution by this extraction process were considered as the surface adsorption contents by a microbial consortium133031. Samples were measured the concentration of n -hexadecane at day 1, 2, 3, 4, 5, 6, 7 respectively.…”

Section: Methodsmentioning

confidence: 99%

Metabolic pathway for a new strain Pseudomonas synxantha LSH-7′: from chemotaxis to uptake of n-hexadecane

Long

Bao

et al. 2017

Sci Rep

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“…() observed that bioadsorption of heat‐killed cells was related to carbon‐normalized partition coefficients. Ding, Chen, and Zhu () explored the respective partition coefficients of PHE and PYR and pointed out that removal of PAHs by heat‐killed bodies of Phanerochaete chrysosporium was only attributed to the mechanism of bioadsorption. These findings indicated the rapid adsorption of PHs by heat‐killed mycelial pellets.…”

Section: Resultsmentioning

confidence: 99%

Removal and biodegradation of different petroleum hydrocarbons using the filamentous fungus Aspergillus sp. RFC‐1

Al‐Hawash

Zhang

2018

MicrobiologyOpen

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Petroleum pollution inevitably occurs at any stage of oil production and exerts a negative impact on the environment. Some microorganisms can degrade petroleum hydrocarbons (PHs). Polluted sludge of Rumaila oil field was use to isolate the highly efficient hydrocarbon‐degrading fungal strain. Aspergillus sp. RFC‐1 was obtained and its degradation ability for petroleum hydrocarbons was evaluated through surface adsorption, cell uptake, hydrophobicity, surface tension, biosurfactant production, and emulsification activity. In addition, the degradation mechanism was investigated. The results indicated the strain RFC‐1 showed high removal activity for PHs, including biodegradation, adsorption, and emulsifiability. On the day 7 of incubation, the removal efficiencies of crude oil, naphthalene (NAP), phenanthrene (PHE), and pyrene (PYR) reached 60.3%, 97.4%, 84.9%, and 90.7%, respectively. Biodegradation efficiencies of crude oil, NAP, PHE, and PYR were 51.8%, 84.6%, 50.3%, and 55.1%, respectively. Surface adsorption and cell absorption by live mycelial pellets followed a decreasing order: PYR ≥ PHE > NAP > crude oil. Adsorption by heat‐killed mycelial pellets increased within 40 and 10 min for crude oil and PAHs, respectively, and remained constant thereafter. Effects of cell surface hydrophobicity, surface tension, and emulsification index were discussed. Intra‐ and extracellular enzymes of strain RFC‐1 played important roles in PHs degradation. The strain RFC‐1 is a prospective strain for removing PHs from aqueous environments.

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“…For instance, Boonchan et al (2000), Acevedo et al (2011), andDing et al (2013) have used successfully white-rot fungi in the biodegradation of PAHs. Some others have demonstrated the efficiency of these fungal group in biodegradation of petroleum derivatives in the environment (Ting et al, 2011;Yanto and Tachibana, 2014;Khan et al, 2015).…”

Section: Evaluation Of Fungal Bioaugmentation and The Removal Of Petrmentioning

confidence: 99%

The effects of white-rot fungi Trametes versicolor and Bjerkandera adusta on microbial community structure and functional genes during the bioaugmentation process following biostimulation practice of petroleum contaminated soil

Shahi

Aydın

İnce

et al. 2016

International Biodeterioration & Biodegradation

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Biosorption and biodegradation of polycyclic aromatic hydrocarbons by Phanerochaete chrysosporium in aqueous solution

Cited by 46 publications

References 33 publications

Metabolic pathway for a new strain Pseudomonas synxantha LSH-7′: from chemotaxis to uptake of n-hexadecane

Metabolic pathway for a new strain Pseudomonas synxantha LSH-7′: from chemotaxis to uptake of n-hexadecane

Removal and biodegradation of different petroleum hydrocarbons using the filamentous fungus Aspergillus sp. RFC‐1

The effects of white-rot fungi Trametes versicolor and Bjerkandera adusta on microbial community structure and functional genes during the bioaugmentation process following biostimulation practice of petroleum contaminated soil

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