Remote Sensing Using an Airborne Biosensor

Ligler, Frances S.; Anderson, George P.; Davidson, Peggy T.; Foch, Richard J.; Ives, Jeffrey T.; King, Keeley D.; Page, Greg; Stenger, David A.; Whelan, James P.

doi:10.1021/es970991p

Cited by 58 publications

(38 citation statements)

References 10 publications

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“…During development of a portable remotely operated fiber optic biosensor system for detecting aerosolized bacteria (19), research carried out by the U.S. Naval Research Laboratory revealed the potential of an immunoassay system used to sample relatively large volumes of air.…”

mentioning

confidence: 99%

A New Method To Monitor Airborne Inoculum of the Fungal Plant Pathogens Mycosphaerella brassicicola and Botrytis cinerea

Kennedy¹,

Wakeham²,

Byrne³

et al. 2000

Appl Environ Microbiol

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We describe a new microtiter immunospore trapping device (MTIST device) that uses a suction system to directly trap air particulates by impaction in microtiter wells. This device can be used for rapid detection and immunoquantification of ascospores of Mycosphaerella brassicicola and conidia of Botrytis cinerea by an enzymelinked immunosorbent assay (ELISA) under controlled environmental conditions. For ascospores of M. brassicicola correlation coefficients (r 2 ) of 0.943 and 0.9514 were observed for the number of MTIST device-impacted ascospores per microtiter well and the absorbance values determined by ELISA, respectively. These values were not affected when a mixed fungal spore population was used. There was a relationship between the number of MTIST device-trapped ascospores of M. brassicicola per liter of air sampled and the amount of disease expressed on exposed plants of Brassica oleracea (Brussels sprouts). Similarly, when the MTIST device was used to trap conidia of B. cinerea, a correlation coefficient of 0.8797 was obtained for the absorbance values generated by the ELISA and the observed number of conidia per microtiter well. The relative collection efficiency of the MTIST device in controlled plant growth chambers with limited airflow was 1.7 times greater than the relative collection efficiency of a Burkard 7-day volumetric spore trap for collection of M. brassicicola ascospores. The MTIST device can be used to rapidly differentiate, determine, and accurately quantify target organisms in a microflora. The MTIST device is a portable, robust, inexpensive system that can be used to perform multiple tests in a single sampling period, and it should be useful for monitoring airborne particulates and microorganisms in a range of environments.

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mentioning

confidence: 99%

A New Method To Monitor Airborne Inoculum of the Fungal Plant Pathogens Mycosphaerella brassicicola and Botrytis cinerea

Kennedy¹,

Wakeham²,

Byrne³

et al. 2000

Appl Environ Microbiol

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“…For example, the Raptor (Figure 3) is an automated fiber-optic biosensor that weighs 12 lbs, which originated in the lab of Ligler and Anderson at the Naval Research Laboratory (8). The system is based on a sandwich immunoassay in which capture antibodies are immobilized by passive adsorption onto a 4-cm fiber-optic polystyrene waveguide with a blackened distal end.…”

Section: Future Strategiesmentioning

confidence: 99%

“…A prototype system was deployed on a remotely piloted airplane that collected aerosols in flight, identified the collected bacteria, and transmitted the data to an operator (8). An alternative design by the Ligler group uses arrays of capture antibodies, thereby greatly increasing the number of simultaneous assays that are conducted by the Raptor (9).…”

Section: Corbismentioning

confidence: 99%

Peer Reviewed: Biological Warfare Detection

Walt¹,

Franz²

2000

Anal. Chem.

127

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“…Conversely, UASs have also been used for air sampling and environmental analysis. Ligler et al (1998) integrated a biosensor system on a small and remotely piloted airplane in order to collect aerosolized bacteria in flight, identify them, and transmit the data on the ground. Nevertheless this system required human intervention for control and planning proposes.…”

Section: Introductionmentioning

confidence: 99%

“…The onboard fluorimeter (controlled by the ground station), provides real-time detection of fluorescent particles in the atmosphere. This fluorescence technique has been used by several researchers in the community for particle detection (Ligler et al, 1998;Sanders, Rodriguez, & Greenbaum, 2001;Zhang et al, 1993). The use of particle counters/sizers onboard UASs provides an excellent method of real-time identification of particles of known sizes in the atmosphere if the particles are present in large concentrations.…”

Section: Introductionmentioning

confidence: 99%

Development of an autonomous unmanned aerial system to collect time‐stamped samples from the atmosphere and localize potential pathogen sources

Gonzalez

Castro

Narayan

et al. 2011

Journal of Field Robotics

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This paper presents the hardware development and testing of a new concept for air sampling via the integration of a prototype spore trap onboard an unmanned aerial system (UAS). We propose the integration of a prototype spore trap onboard a UAS to allow multiple capture of spores of pathogens in single remote locations at high or low altitude, otherwise not possible with stationary sampling devices. We also demonstrate the capability of this system for the capture of multiple time-stamped samples during a single mission. Wind tunnel testing was followed by simulation, and flight testing was conducted to measure and quantify the spread during simulated airborne air sampling operations. During autonomous operations, the onboard autopilot commands the servo to rotate the sampling device to a new indexed location once the UAS vehicle reaches the predefined waypoint or set of waypoints (which represents the region of interest). Time-stamped UAS data are continuously logged during the flight to assist with analysis of the particles collected. Testing and validation of the autopilot and spore trap integration, functionality, and performance is described. These tools may enhance the ability to detect new incursions of spores. C 2011 Wiley Periodicals, Inc.

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Remote Sensing Using an Airborne Biosensor

Cited by 58 publications

References 10 publications

A New Method To Monitor Airborne Inoculum of the Fungal Plant Pathogens Mycosphaerella brassicicola and Botrytis cinerea

A New Method To Monitor Airborne Inoculum of the Fungal Plant Pathogens Mycosphaerella brassicicola and Botrytis cinerea

Peer Reviewed: Biological Warfare Detection

Development of an autonomous unmanned aerial system to collect time‐stamped samples from the atmosphere and localize potential pathogen sources

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