Remodelling of the Corneal Stroma After Lamellar Keratoplasty

Quantock, Andrew J.; Kratz-Owens, Katherine; Leonard, D.W.; Meek, Keith Michael Andrew

doi:10.1097/00003226-199401000-00005

Cited by 11 publications

(4 citation statements)

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“…Previously, the lack of transparency following corneal wounding has been ascribed to increased corneal thickness and increased diameter of collagen fibrils 5 and/or a poor fibrillar organization of the regenerated stroma 6,7 . Studies using transmission electron microscopy (TEM) have also shown an abnormal organization of corneal collagen fibrils during wound healing 8,9 .…”

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Organization of corneal collagen fibrils during the healing of trephined wounds in rabbits

Connon

Meek

2003

Wound Repair Regeneration

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The purpose of this study was to investigate the structure and organization of collagen during the healing of penetrating rabbit corneal wounds. Full-thickness wounds were produced in both corneas of six New Zealand White rabbits with a 2-mm trephine. After 5, 10, and 16 months, wounds were examined using transmission electron microscopy and wide-angle synchrotron X-ray diffraction, which allowed the quantification of the relative amount of collagen and fibril orientation in different parts of the cornea. The intensity of the diffraction patterns taken from the wound edge was much higher than patterns collected at the wound center, indicating a greater amount of collagen near the wound edge. This collagen was shown to have a preferred orientation, forming a circular pattern around the wound. As the wounds healed, the amount of preferentially aligned collagen decreased and lamella formation and ordered fibrillar arrangement increased as shown by electron microscopy. The orientation of collagen fibrils in healing penetrating corneal wounds is not random. Instead, fibrils tend to be circularly arranged within and without the wound, gradually becoming more normal over time. We speculate that collagen is artefactually displaced during trephination, resulting in an abnormal alignment of the fibrils surrounding the wound edge, and that this influences the wound healing process.

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Organization of corneal collagen fibrils during the healing of trephined wounds in rabbits

Connon

Meek

2003

Wound Repair Regeneration

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“…Uniform collagen fibril diameter, uniform inter‐fibrillar distance and orthogonal alignment of collagen fibres are necessary for corneal transparency (Knupp et al ., ; Hassell and Birk, ). Loss of transparency in the wounded cornea is caused by increased corneal thickness and increased diameter of collagen fibrils as well as poor fibrillar organization in the regenerated stroma (Quantock et al ., ). In this study, the corneas implanted with BIOSHEETs were thick and hazy immediately after implantation.…”

Section: Discussionmentioning

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In-body tissue-engineered collagenous connective tissue membranes (BIOSHEETs) for potential corneal stromal substitution

Takiyama

Mizuno

Iwai

et al. 2013

J Tissue Eng Regen Med

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There is a severe shortage of donor cornea for transplantation in many countries. Collagenous connective tissue membranes, named BIOSHEETs, grown in vivo were successfully implanted in rabbit corneal stroma for in vivo evaluation of their suitability as a corneal stromal substitute to solve this global donor shortage. BIOSHEETs were prepared by embedding silicone moulds into dorsal subcutaneous pouches in rabbits for 1 month and stored in glycerol. After re-swelling in saline and trephining, disk-shaped BIOSHEETs (4 mm diameter) were allogeneically implanted into stromal pockets prepared in the right cornea of seven rabbits. Clinical tests for corneal thickness and transparency, and tissue analyses were performed. Because the BIOSHEETs (thickness, 131 ± 14 µm) obtained were opaque immediately after implantation, the transparency of the cornea decreased. The total thickness of the BIOSHEET-implanted cornea increased from 364 ± 21.0 µm to 726 ± 131 µm. After 4 weeks' implantation, the thickness of the cornea stabilized (493 ± 80 µm at 4 weeks and 447 ± 46 µm at 8 weeks). The transparency of the cornea increased progressively with time of implantation. The random orientation of collagen fibrils in the original BIOSHEETs tended to be homogeneous, similar to that of the native stroma. No inflammatory cells accumulated and fibroblast-like cells infiltrated the implant. The BIOSHEETs showed high biocompatibility with stromal tissues; however, further studies are needed to test its functional aspects. Although this research is only intended as a proof of concept, BIOSHEETs may be considered a feasible corneal stromal replacement, especially for treating visual impairment caused by stromal haze. Copyright © 2013 John Wiley & Sons, Ltd.

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“…Depending on the degree of order in the structure, different levels of interference between the scattered rays will occur. X-ray diffraction techniques have been applied to samples of diseased human corneas, as well as to corneas post-refractive surgery [8,[11][12][13].…”

Section: Confocal Microscopymentioning

confidence: 99%