Remodelling chromatin with RNA

Willard, Huntington F.; Salz, Helen K.

doi:10.1038/386228a0

Cited by 30 publications

(10 citation statements)

References 12 publications

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“…The presence of ncRNAs has been described in eukaryotic and prokaryotic systems (Akhtar et al 2000;Amrein and Axel 1997;Lee et al 1999;Panning and Janeisch 1998;Storz 2002;Stuckenholz et al 2003;Willard and Salz 1997). Dosage compensation is one of the most intensively studied processes concerning ncRNA.…”

Section: Introductionmentioning

confidence: 98%

“…In mammalian systems, the Xist RNA is crucial for X inaction in females. The RNA physically coats the inactivated X chromosome (Barr body) and is probably involved in changes in chromatin architecture (Panning and Janeisch 1998;Willard and Salz, 1997). It is interesting that Xist is apparently regulated by another ncRNA, an antisense RNA called Tsix (Lee et al 1999).…”

Section: Introductionmentioning

confidence: 99%

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Overexpression of Zm401, an mRNA-like RNA, has distinct effects on pollen development in maize

Dai

et al. 2007

Plant Growth Regul

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We previously identified a 0.7 Kb cDNA fragment of Zm401, a novel pollen-specific gene in maize (Zea mays). However, little information is known about the function of Zm401 in pollen development. The full-length of Zm401 cDNA was amplified by 5 0 RACE and 3 0 RACE and both sequence analysis and in vitro translation of Zm401 showed that it belonged to an mRNA-like non-coding gene. To analyze its possible biological roles in pollen development, the Zm401 cDNA was overexpressed in transgenic maize under the control of a pollen specific promoter Zm13 or a CaMV 35S promoter. RT-PCR and RNA gel blot analysis indicated that the expression level of Zm401 in leaves and anthers of transgenic plants was much higher than that of non-transformants. Compared with the non-transformed maize, transgenic maize showed distinct phenotypes, such as abnormal tassels and degenerate anthers. The histological observation showed that the development of pollen grains and anthers in transgenic plants were abnormal. These abnormalities include delayed degradation of tapetum, asynchronous fusion of pollen sacs, and aborted pollen grain development. Furthermore, the pollen viability in six transgenic plants ranged from 1.24% to 6.63%. The reduced pollen viability cosegregated with the transgene in a selfed progeny. These results suggest that Zm401 is involved in the regulation of pollen development. This article demonstrated Zm401, as a non-coding RNA, plays an essential role in pollen development.

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Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Overexpression of Zm401, an mRNA-like RNA, has distinct effects on pollen development in maize

Dai

et al. 2007

Plant Growth Regul

View full text Add to dashboard Cite

show abstract

“…In mammalian systems, the Xist RNA is critical for X inactivation in females. The RNA physically coats the inactivated X chromosome (Barr body) and is probably involved in changes in chromatin architecture (Willard and Salz, 1997;Panning and Jaenisch, 1998). It is interesting that Xist is apparently regulated by another ncRNA, an antisense RNA called Tsix (Lee et al, 1999).…”

mentioning

confidence: 99%

Identification and Analysis of Arabidopsis Expressed Sequence Tags Characteristic of Non-Coding RNAs

MacIntosh

2001

Plant Physiology

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“…[55][56][57] FISH DETECTION OF mRNA FISH can be used to detect single stranded messenger RNA in situ. [58][59][60] This is a potentially important application of the FISH technique because it provides direct visual evidence of gene expression from a particular chromosome. It is technically demanding at present but may have applications in the future in a …”

Section: Sensitivitymentioning

confidence: 99%