Demystified ... FISH

Waters, J. J.; Barlow, A. L.; Gould, C. P.

doi:10.1136/mp.51.2.62

Cited by 15 publications

(4 citation statements)

References 75 publications

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“…FISH allows analysis of chromosomal disturbances even in samples in bad condition. Drawbacks of FISH include higher price, inability to value the pathomorphological alterations and the long time to count FISH signals by fl uorescence microscopy [26,27].…”

Section: Discussionmentioning

confidence: 99%

Loss of P16 in Esophageal Adenocarcinoma Detected by Fluorescence in situ Hybridization and Immunohistochemistry

Kotzev

Kamenova

2017

Acta Medica Bulgarica

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Section: Discussionmentioning

confidence: 99%

Loss of P16 in Esophageal Adenocarcinoma Detected by Fluorescence in situ Hybridization and Immunohistochemistry

Kotzev

Kamenova

2017

Acta Medica Bulgarica

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“…Endogenous DNA is largely nuclear-for example, chromosomes and individual genes, and these are most frequently detected by FISH, 6 which is be the subject of a further article in this series. 11 Non-fluorescent methods can also be used for larger targets and give good morphological correlation. Endogenous cytoplasmic DNA-for example, mitochondrial DNA, can also be examined but this is an uncommon application.…”

Section: Applicationsmentioning

confidence: 99%

Demystified ... in situ hybridisation

Herrington

1998

Molecular Pathology

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“…The principal test system is based on the actions and sequences associated with T7 RNA polymerase (RNAP), which is the best characterized member of a family of RNAPs that includes most of the bacteriophage-encoded RNAPs as well as the mitochondrial RNAPs [28][29][30][31]. Previous experiments indicated that T7 RNA polymerase can actively incorporate fluorochrome labeled ribonucleoside triphosphates into nascent transcripts [32], most readily with rhodamine dyes (Rhodamine Green, Tetramethylrhodamine).…”

Section: Introductory Statementmentioning

confidence: 99%

Transchip: Single-molecule detection of transcriptional elongation complexes

Schwartz

2007

Analytical Biochemistry

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A new single molecule system -Transchip -was developed for analysis of transcription products at their genomic origins. The bacteriophage T7 RNA polymerase and its promoters were used in a model system, and resultant RNAs were imaged and detected at their positions along single template DNA molecules. This system, Transchip, has drawn from critical aspects of Optical Mapping, a single molecule system that enables the construction of high resolution, ordered restriction maps of whole genomes from single DNA molecules. Through statistical analysis of hundreds of single molecule template/transcript complexes, Transchip enables analysis of the locations and strength of promoters, the direction and processivity of transcription reactions and termination of transcription. These novel results suggest that the new system may serve as a high-throughput platform to investigate transcriptional events on a large, genome-wide scale. KeywordsSingle molecule; transcription; elongation complex; T7 RNA polymerase; promoter mapping; automated fluorescence microscopy Introductory StatementThis study aims to develop an integrated system for transcriptional analysis of single template DNAs, with the ultimate goal being analysis of transcriptional regulation for genes on a whole genome basis. Large template DNA molecules can be queried to probe for regulatory elements and for transcriptional events. The Transchip system described in this manuscript aims to produce large and meaningful datasets for transcriptional analysis, all based around single molecule measurements. Experiments based on single molecules are direct and rapid, and can generate a read-out of thousands of data points in a short time period.The development of single molecule approaches to biochemical analysis has enabled a broad range of techniques that visualize the details of and that elegantly probe individual steps involved in transcription [1][2][3][4][5][6]. These elegant biochemical studies performed on single molecules have brought numerous insights into the mechanisms of transcription, but they do not lend themselves to high throughout transcriptional analysis. Visualization and mapping of RNAs transcribed from single template molecules has long been possible by electron + To whom correspondence should be addressed: dcschwartz@wisc.edu, 608-265-0546; 608-265-6743 (FAX) # current position, Amgen, Inc., One

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Demystified ... FISH

Cited by 15 publications

References 75 publications

Loss of P16 in Esophageal Adenocarcinoma Detected by Fluorescence in situ Hybridization and Immunohistochemistry

Loss of P16 in Esophageal Adenocarcinoma Detected by Fluorescence in situ Hybridization and Immunohistochemistry

Demystified ... in situ hybridisation

Transchip: Single-molecule detection of transcriptional elongation complexes

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