Remission of Diabetes Following Bariatric Surgery: Plasma Proteomic Profiles

Insenser, María; Vilarrasa, Núria; Vendrell, Joan; Escobar-Morreale, Héctor F.

doi:10.3390/jcm10173879

Cited by 10 publications

(7 citation statements)

References 51 publications

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“…The DM2 resolved with no drug treatment after the bariatric surgery in 75% of the patients who had this metabolic disorder, which was accompanied by de ned changes in intestinal microbial patterns, as previously reported [7,15,50,51]. The contribution of the intestinal microbiota in resolving DM2 is still controversial, whereas biliary salts appear to play a relevant role [32].…”

Section: Discussionsupporting

confidence: 54%

“…Bariatric surgery not only affects bacterial gut composition [4,5], with RYGB causing deeper changes, but also its overall functionality [1, 6]. Beyond the bacterial composition, scienti c research is currently focused on microbial metabolism, particularly the mechanisms involved in DM2 remission [7], as well as on the microbiome functionality evolution [8], including short-chain fatty acids (SCFAs) [9] and other microbial products related to the excess weight loss. The aim of the present study was to monitor the impact of a preoperative crash diet and bariatric surgery on bacterial gut microbiota composition and functionality in severe obesity, to compare the RYGB and SG approaches, and to apply a Lasso predictive analysis based on multiple linear regressions to detect potential markers of excess weight loss.…”

mentioning

confidence: 99%

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Fecal Metabolome and Bacterial Composition in Severe Obesity: Impact of Diet and Bariatric Surgery

Salazar

Ponce-Alonso

Garriga

et al. 2022

Preprint

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Background: The aim of this study was to monitor the impact of a preoperative diet and bariatric surgery on the bacterial gut microbiota composition and functionality in severe obesity and to compare sleeve gastrectomy (SG) versus Roux-en-Y gastric bypass (RYGB). The study also aimed to incorporate big data analysis for the omics results and machine learning by a Lasso-based analysis to detect the potential markers for excess weight loss.Methods: Forty patients who underwent bariatric surgery were recruited (14 underwent SG, and 26 underwent RYGB). Each participant contributed 4 fecal samples (baseline, post-diet, 1 month after surgery and 3 months after surgery). The bacterial composition was determined by 16S rDNA massive sequencing using MiSeq (Illumina). Metabolic signatures associated to fecal concentrations of short-chain fatty acids, amino acids, biogenic amines, gamma-aminobutyric acid and ammonium were determined by gas and liquid chromatography. Orange 3 software was employed to correlate the variables, and a Lasso analysis was employed to predict the weight loss at the baseline samples.Results: A correlation between Bacillota (formerly Firmicutes) abundance and excess weight was observed only for the highest body mass indexes. The crash diet had little impact on composition and targeted metabolic activity. RYGB had a deeper impact on bacterial composition and putrefactive metabolism than SG, although the excess weight loss was comparable in the two groups. Significantly higher ammonium concentrations were detected in the feces of the RYGB group. Conclusions: We detected individual signatures of composition and functionality, rather than a gut microbiota characteristic of severe obesity, with opposing tendencies for almost all measured variables in the two surgical approaches. The gut microbiota of the baseline samples was not useful for predicting excess weight loss after the bariatric process.

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Section: Discussionsupporting

confidence: 54%

mentioning

confidence: 99%

Fecal Metabolome and Bacterial Composition in Severe Obesity: Impact of Diet and Bariatric Surgery

Salazar

Ponce-Alonso

Garriga

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…The DM2 resolved with no drug treatment after the bariatric surgery in 75% of the patients who had this metabolic disorder, which was accompanied by defined changes in intestinal microbial patterns, as previously reported. 7 , 15 , 50 , 51 The contribution of the intestinal microbiota in resolving DM2 is still controversial, whereas biliary salts appear to play a relevant role. 32 We were unable to identify a correlation between bacterial genera abundance and DM2 resolution in 75% of our patients (9/12).…”

Section: Discussion (Bray Curtis!)mentioning

confidence: 99%

“…Bariatric surgery not only affects bacterial gut composition, 4 , 5 with RYGB causing deeper changes, but also its overall functionality. 1 , 6 Beyond the bacterial composition, scientific research is currently focused on microbial metabolism, particularly the mechanisms involved in DM2 remission, 7 as well as on the microbiome functionality evolution, 8 including short-chain fatty acids (SCFAs) 9 and other microbial products related to the excess weight loss. The aim of the present study was to monitor the impact of a preoperative low-calorie diet and bariatric surgery on bacterial gut microbiota composition and functionality in severe obesity, to compare the RYGB and SG approaches, and to apply a Lasso predictive analysis based on multiple linear regressions to detect potential markers of excess weight loss.…”

Section: Introductionmentioning

confidence: 99%

Fecal Metabolome and Bacterial Composition in Severe Obesity: Impact of Diet and Bariatric Surgery

et al. 2022

View full text Add to dashboard Cite

The aim of this study was to monitor the impact of a preoperative low-calorie diet and bariatric surgery on the bacterial gut microbiota composition and functionality in severe obesity and to compare sleeve gastrectomy (SG) versus Roux-en-Y gastric bypass (RYGB). The study also aimed to incorporate big data analysis for the omics results and machine learning by a Lasso-based analysis to detect the potential markers for excess weight loss. Forty patients who underwent bariatric surgery were recruited (14 underwent SG, and 26 underwent RYGB). Each participant contributed 4 fecal samples (baseline, post-diet, 1 month after surgery and 3 months after surgery). The bacterial composition was determined by 16S rDNA massive sequencing using MiSeq (Illumina). Metabolic signatures associated to fecal concentrations of short-chain fatty acids, amino acids, biogenic amines, gamma-aminobutyric acid and ammonium were determined by gas and liquid chromatography. Orange 3 software was employed to correlate the variables, and a Lasso analysis was employed to predict the weight loss at the baseline samples. A correlation between Bacillota (formerly Firmicutes) abundance and excess weight was observed only for the highest body mass indexes. The low-calorie diet had little impact on composition and targeted metabolic activity. RYGB had a deeper impact on bacterial composition and putrefactive metabolism than SG, although the excess weight loss was comparable in the two groups. Significantly higher ammonium concentrations were detected in the feces of the RYGB group. We detected individual signatures of composition and functionality, rather than a gut microbiota characteristic of severe obesity, with opposing tendencies for almost all measured variables in the two surgical approaches. The gut microbiota of the baseline samples was not useful for predicting excess weight loss after the bariatric process.

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“…Recent studies reported that C-reactive protein as an acute phase reactant has close relationship with type 2 diabetes [28], as well as the inflammatory marker of cardiovascular diseases. After bariatric surgery, plasma proteomic alternations of remission diabetes proteins participated in acute phase response, platelet degranulation, fibrinolysis, and coagulation in obese patients with T2D [29]. Cardiovascular and metabolic diseases including atherosclerosis and diabetes have a common hallmark endothelial dysfunction, with impaired endothelium-dependent vasodilation, heightened oxidative stress, and chronic inflammation [30].…”

Section: Discussionmentioning

confidence: 99%

DIA-PRM Proteomic Analysis of Phlegm-Dampness Constitution with Glucolipid Metabolic Disorders by the Intervention of Hua Tan Qu Shi Recipe

Sun

et al. 2022

BioMed Research International

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Background. Phlegm-dampness constitution as one of nine constitutions in traditional Chinese medicine (TCM) has been a high risk factor for glucolipid metabolic disorders (GLMD). Based on our previous findings, Hua Tan Qu Shi recipe (HTQSR) could effectively improve metabolic indicators of GLMD by targeting on phlegm-dampness constitution. However, the proteomic mechanisms of GLMD with the treatment of HTQSR targeting on phlegm-dampness constitution remain unknown. Methods. Clinical participants from phlegm-dampness constitution with the prediabetic state (T), phlegm-dampness constitution with marginally elevated blood lipids (Z), and phlegm-dampness constitution before sickness (W) were included in this study, who orally took HTQSR for 12 weeks and, respectively, marked AT, AZ, and AW. Data-independent acquisition (DIA) and parallel reaction monitoring (PRM) were performed to identify the differential proteins; then, Venn analysis was used to investigate coexpressed and coregulated proteins. In addition, ingenuity pathway analysis (IPA) software was utilized to explore the related pathways and diseases and biofunctions. Results. LXR/RXR activation, acute phase response signaling, and production of nitric oxide and reactive oxygen species in macrophages were obviously activated between the T and AT groups, as well as the Z and AZ groups. In contrast, these three pathways were inhibited between the W and AW groups. Importantly, one coexpressed and coregulated differential protein, B2MG, was validated by PRM among all groups. Conclusions. This work firstly reported the underlying proteomic mechanisms of GLMD with the treatment of HTQSR targeting on phlegm-dampness constitution, indicating that intervention of phlegm-dampness constitution might be a novel strategy for the preventive treatment of GLMD.

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Remission of Diabetes Following Bariatric Surgery: Plasma Proteomic Profiles

Cited by 10 publications

References 51 publications

Fecal Metabolome and Bacterial Composition in Severe Obesity: Impact of Diet and Bariatric Surgery

Fecal Metabolome and Bacterial Composition in Severe Obesity: Impact of Diet and Bariatric Surgery

Fecal Metabolome and Bacterial Composition in Severe Obesity: Impact of Diet and Bariatric Surgery

DIA-PRM Proteomic Analysis of Phlegm-Dampness Constitution with Glucolipid Metabolic Disorders by the Intervention of Hua Tan Qu Shi Recipe

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