Remineralization of Demineralized Enamel and Dentine Using 3 Dentifrices—An InVitro Study

Abstract

Objectives: To monitor the electrical resistance of artificially demineralized enamel and root dentine after exposure to different fluoridated dentifrices and, using transversal microradiography, to quantify remineralization. Materials and methods: This in-vitro blind investigation used 20 extracted teeth (four groups of five each). Each group was exposed to one test dentifrice [Colgate PreviDent (5000 ppm F), Colgate Winterfresh gel (1100 ppm F), Fluocaril Bi-Fluoré (2500 ppm F) and placebo (without fluoride)] three times daily for three minutes for 4 weeks. In between exposure to the test dentifrices, teeth were stored in a saliva storage solution. An Electrical Caries Monitor measured the electrical resistance at baseline and during the four-week test period at weekly intervals. The measurements were log transformed and Duncan’s multiple range test applied. Remineralization was quantified using transversal microradiography. Results: Log mean (SD) electronic carries monitor (ECM) measurements in enamel at baseline and after 4 weeks of exposure to the test dentifrices were 4.07(1.53) and 3.87(0.90) (Placebo-Fluocaril), 4.11(1.86) and 4.64(1.43) (Colgate Winterfresh gel), 4.81(0.9) and 4.21(1.20) (Fluocaril Bi-Fluoré), and 4.60(0.88) and 3.76(0.9) (Colgate PreviDent). Corresponding measurements in dentine were 2.13(0.89) and 3.06(0.87) (Placebo-Fluocaril), 1.87(0.63) and 2.88(1.32) (Colgate Winterfresh gel), 2.47(1.20) and 1.65(0.60) (Fluocaril), and 2.16(0.00), and 2.34(1.07) for Colgate PreviDent. Lesion depth (µm) after microradiography in enamel was 100.1 (Placebo), 50.6 (Colgate Winterfresh gel), and 110.2 (Fluocaril, and 97.1 (Colgate PreviDent), and corresponding values in dentine were 169.7, 154.8, 183.7, and 153.5. The correlation of ECM and microradiographic parameters was negative (p < 0.05). Conclusion: Exposure of artificially demineralized enamel and root dentine to fluoridated dentifrices and saliva storage solution resulted in remineralization as follows: Colgate Winterfresh > Colgate PreviDent > Placebo-Fluocaril > Fluocaril Bi-Fluoré. Remineralization in teeth of the Placebo dentifrice group may be attributed to the presence of calcium and phosphate ions in the saliva storage solution.

Comparative Evaluation of Influence of Nd:YAG Laser (1064 nm) and 980 nm Diode Laser on Enamel around Orthodontic Brackets: An In Vitro Study

“…However, in patients with xerostomia, Sjogren’s syndrome, radiation caries, and older adults who are home-bound due to impaired physical and mental disabilities and have difficulty in carrying out their daily oral hygiene regimen, brushing once daily with a high-fluoride dentifrice would be a more pragmatic approach. The findings may also have implications for the management of white spots in high-risk orthodontic patients [ 58 ].…”

Molar Incisor Hypomineralization in Children with Intellectual Disabilities

“…Possible preventive therapies for the treatment of MIH could be beneficial. Some remineralizing agents have been proposed for the treatment of enamel hypomineralization, in addition to conventional fluoride-based therapies, biomimetic hydroxyapatite, and casein phosphopeptide-amorphous calcium phosphate have also been recently introduced and have shown promising results [ 37 , 38 , 39 ]. Oral health promotion programs should be aimed at centers and schools for children with intellectual disabilities and should include regular use of oral health services, oral hygiene education, and dietary counseling, which is especially important when a child has MIH, a major risk factor for caries.…”

Remineralization of Early Carious Lesion Using Natural Agents Versus Bioadhesive Polymers

“…Although enamel is a hard and dense material, it possesses local differences in porosity and acid solubility; acids can penetrate deep into the enamel and dissolve tooth minerals locally, rather than dissolving the enamel layer by layer. Therefore, remineralization of early enamel lesions is of great value to protect other dental tissues (19) .…”