Remapping of the stripe rust resistance gene Yr10 in common wheat

Abstract

Yr10 is an important gene to control wheat stripe rust, and the search for Yr10 needs to be continued. Wheat stripe rust or yellow rust is a devastating fungal disease caused by Puccinia striiformis f. sp. tritici (Pst). Host disease resistance offers a primary source for controlling wheat stripe rust. The stripe rust resistance gene Yr10 confers the race-specific resistance to most tested Pst races in China including CYR29. Early studies proposed that Yr10 was a nucleotide-binding site, leucine-rich repeat gene archived as GenBank accession AF149112 (hereafter designated the Yr10 candidate gene or Yr10 ). In this study, we revealed that 15 Chinese wheat cultivars positive for Yr10 are susceptible to CYR29. We then expressed the Yr10 cDNA in the common wheat 'Bobwhite'. The Yr10 -cDNA positive transgenic plants were also susceptible to CYR29. Thus, it is highly unlikely that Yr10 corresponds to the Yr10 resistance gene. Using the Yr10 donor 'Moro' and the Pst-susceptible wheat 'Huixianhong', we generated two F populations that displayed a single Mendelian segregation on the Yr10 gene, and used them to remap the Yr10 gene. Six markers were placed in the Yr10 region, with the Yr10 gene now mapping about 1.2-cM proximal to the Yr10 locus and the Xsdauw79 marker is completely linked to the Yr10 locus. Apparently, the Yr10 gene has not yet been identified. Fine mapping and positional cloning of Yr10 is important for gene pyramiding for stripe rust resistance in wheat.

Isochorismate‐based salicylic acid biosynthesis confers basal resistance to Fusarium graminearum in barley

“…The maize Ubiquitin ( Ubi ) promoter (Christensen et al ., ) is a constitutive promoter widely used in research. The wheat Wks1 promoter (Fu et al ., ), on the other hand, is an alternative promoter that has been successfully used to express transgenes (Yuan et al ., ). All of the remaining constructs derived from the ICS and PAL genes were based on the Ubi promoter.…”

Genome-Wide Association Study for Multiple Biotic Stress Resistance in Synthetic Hexaploid Wheat

“…The identification of underlying genes with annotations related to the trait provides further reliability for the MTAs identified. For instance, four genes (TraesCS4D01G096900: Lr, TraesCS7A01G517300: Lr, TraesCS4B01G007000: Sr, and TraesCS3A01G495400: Lr) were annotated as the leucine-rich repeat protein family, which was previously reported to have a potential role in Yr [38,39] and Sr resistance [40]. Similarly, five genes (TraesCS2A01G076800: Yr, TraesCS7B01G473300: Yr, TraesCS1A01G325300: Sr, TraesCS6B01G394600: Yr, and TraesCS5B01G199700: Sr) were annotated as the F-box family protein, which were reported to have potential role in Lr [41], Sr [42], and Yr [43] resistance in wheat.…”

Over-Expression of Endogenous SUGARWIN Genes Exalted Tolerance against Colletotrichum Infection in Sugarcane

“…Maize ubiquitin ( Ubi-1 ) promoter is contemplated as a better choice compared to CaMV 35S and Adh1 for the constitutive expression of a transgene, particularly in monocots [ 29 ]. It has extensively been used for the transient as well as stable expression of transgenes in several monocots including wheat [ 30 ], maize [ 31 ], rice [ 32 ], sugarcane [ 33 ], and sorghum [ 34 ]. SUGARWIN1 and SUGARWIN2 genes were cloned in pUbiAB vector under maize ubiquitin promoter ( Ubi-1 ) and nos terminator.…”