Remap as a Tool for Preliminary Grapevine Accession Screening

Šimon, S.; Žulj, Marin Mihaljević; Preiner, Darko; Pejić, Ivan; Gaši, Fuad; Malenica, Nenad; Zdunić, Goran

doi:10.17660/actahortic.2010.859.18

“…Retrotransposon microsatellite amplification polymorphism (REMAP) is an efficient technique involving the polymerase chain reaction (PCR) amplification, with a long terminal repeat (LTR) primer and a simple sequence repeat (SSR) or inter-simple sequence repeat (ISSR) primer (Kalendar and Schulman, 2006;Pandotra et al, 2014). REMAP has been efficiently used for cultivar characterizations of many species, including barley (Hordeum vulgare), grapevine (Vitis vinifera L.), olive (Olea europaea L.), and rice (Oryza sativa L.) (Branco et al, 2007;Kalendar et al, 1999;Kaya and Yilmaz-Gokdogan, 2016;Strioto et al, 2019;Šimon et al, 2010). In terms of distinguishing ginger cultivars, REMAP can discriminate all the 92 ginger landraces (with a high variation rate of 96.5 %) in the study of Pandotra et al (2014).…”

Section: Introductionmentioning

confidence: 99%

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2022

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Study areas include cell biology, genomics, microbiology, immunology, molecular biology, biochemistry, embryology, immunogenetics, cell a n d tissue culture, molecular ecology, genetic engineering and biological engineering, bioremediation and biodegradation, bioinformatics, biotechnology regulations, gene therapy, organismal biology, microbial and environmental biotechnology, marine sciences. The JJBS welcomes the submission of manuscripts that meet the general criteria of significance and academic excellence. All articles published in JJBS are peerreviewed. Papers will be published approximately in one to two months after acceptance.

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“…Retrotransposon microsatellite amplification polymorphism (REMAP) is an efficient technique involving the polymerase chain reaction (PCR) amplification, with a long terminal repeat (LTR) primer and a simple sequence repeat (SSR) or inter-simple sequence repeat (ISSR) primer (Kalendar and Schulman, 2006;Pandotra et al, 2014). REMAP has been efficiently used for cultivar characterizations of many species, including barley (Hordeum vulgare), grapevine (Vitis vinifera L.), olive (Olea europaea L.), and rice (Oryza sativa L.) (Branco et al, 2007;Kalendar et al, 1999;Kaya and Yilmaz-Gokdogan, 2016;Strioto et al, 2019;Šimon et al, 2010). In terms of distinguishing ginger cultivars, REMAP can discriminate all the 92 ginger landraces (with a high variation rate of 96.5 %) in the study of Pandotra et al (2014).…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity Analysis Based on Retrotransposon Microsatellite Amplification Polymorphisms (REMAP) for Distinguishing the Ginger Chemotype of Thua Thien Hue (Zingiber officinale Roscoe) from other Vietnamese Ginger Types

2022

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The ginger of Thua Thien Hue ("Hue" for short) is considered by local people as a chemotype that is different from other Vietnamese ginger populations. However, there is no molecular evidence supporting this statement. Thus, our purpose is to find whether there is a genetic difference between Hue's ginger and other ginger types by using Retrotransposon Microsatellite Amplification Polymorphisms (REMAP). The results of our two cluster analyses (Unweighted Pair Group Method with Arithmetic mean (UPGMA) and Principal Coordinates Analysis (PCoA)) proved that they could separate Hue's ginger samples (in Thuy Bieu Ward, Hue City) from other populations. Therefore, according to these genetic analyses, Hue's ginger is markedly different from other ginger types. Based on our results, future studies could be conducted with more Vietnamese ginger DNA samples to provide stronger evidence about the unique genomic features of Hue's ginger.

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“…Native cultivar conservation in Croatia has greatly improved over the last decades through steady collecting, genotyping and characterisation of native varieties [4][5][6][7]. Nowadays, approximately 130 different varieties have been identified as native to Croatia.…”

Section: Introductionmentioning

confidence: 99%

In vitro introduction of healthy and virus-infected genotypes of native Croatian grapevine cultivars

Marković¹,

Preiner

²

,

Bošnjak

³

et al. 2014

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We evaluated the response of eight economically important Croatian grapevine cultivars and studied the impact of their sanitary status on in vitro introduction, by comparing the response of healthy and virus-infected genotypes of one cultivar. Nodal explant survival on three media, M1 (half-strength MS), M2 (full-strength MS) or M3 (full-strength MS with 4.4 µM L−1 benzylaminopurine) was measured after 2 weeks and regrowth after 8 weeks. After 8 weeks, average shoot length and node number were significantly higher on M2 compared to M1 and M3. M3 induced significantly shorter average internode length, compared to M1 and M2. Survival of one healthy and of five cultivar Plavac mali genotypes infected with GFLV, GLRaV-1, GLRaV-3, GLRaV-3+GVA and GLRaV-1+GLRaV-3 was 97.5 and 82.8–87.5%, respectively. Regrowth of the healthy genotype reached 95.5%, but dropped to 5.5–31.4% in infected ones. The healthy genotype showed significantly higher shoot length (6.3 cm) and node number (7.3) compared to infected genotypes, with shoot length between 1.2–2.6 cm and node number between 1.2–3.0. By contrast, internode length was not significantly different between the healthy and the infected genotypes. The present work represents the first successful in vitro introduction for three of the eight native Croatian cultivars studied.

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Remap as a Tool for Preliminary Grapevine Accession Screening

Cited by 3 publications

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Genetic Diversity Analysis Based on Retrotransposon Microsatellite Amplification Polymorphisms (REMAP) for Distinguishing the Ginger Chemotype of Thua Thien Hue (Zingiber officinale Roscoe) from other Vietnamese Ginger Types

In vitro introduction of healthy and virus-infected genotypes of native Croatian grapevine cultivars

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