Reliability of Transcutaneous Measurement of Renal Function in Various Strains of Conscious Mice

Schock-Kusch, Daniel; Geraci, Stefania; Ermeling, Esther; Shulhevich, Yury; Sticht, Carsten; Hesser, Juergen; Stsepankou, Dzmitry; Neudecker, Sabine; Pill, Johannes; Schmitt, Roland; Melk, Anette

doi:10.1371/journal.pone.0071519

Cited by 58 publications

(61 citation statements)

References 25 publications

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“…Sequential measurements within 3 days in the same animal showed a coefficient of variances of 3.0-6.2% 13 . In this study there was no conversion to GFR but the results were expressed and interpreted directly in terms of t 1/2 .…”

Section: Representative Resultsmentioning

confidence: 92%

“…Therefore, sequential measurements within the same animal have been limited by the welfare of the animals and methodological reasons. The proved withinanimal reproducibility of the transcutaneous measurement 13 makes it an ideal method for detecting changes in kidney function due to disease progression or ageing. For the same reason, it can also be extremely useful to study the effectiveness of therapeutic approaches.…”

Section: Discussionmentioning

confidence: 99%

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Transcutaneous Assessment of Renal Function in Conscious Rodents

Pérez

Weinfurter

Gretz

2016

JoVE

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Glomerular filtration rate (GFR) is the gold standard to assess overall kidney function. However, traditional methods to evaluate GFR are cumbersome and time-consuming. In addition, serial blood or urine samples are required, with the associated stress for the experimental animals. A recent technique significantly reduces the investment in time and resources, minimizing the invasiveness and the animal stress, but being equally valid as the traditional approaches. The method measures transcutaneously renal function. Using an optical device and the exogenous renal marker fluorescein isothiocyanate (FITC)-sinistrin, this technique is capable of measuring the elimination kinetics of the marker through the skin. With neither blood nor urine samples nor the associated laboratory assays needed, the results of the transcutaneous measurement are almost instantaneously available. The method has been already validated in different species and successfully applied in several models of renal pathology. Moreover, due to its minimally invasive characteristics, it is suitable for sequential measurements within the same animal. Here is provided a detailed protocol to carry out the transcutaneous assessment of renal function in rodents.

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Section: Representative Resultsmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Transcutaneous Assessment of Renal Function in Conscious Rodents

Pérez

Weinfurter

Gretz

2016

JoVE

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“…This could attemper the consequences of the above altered vascular tone. Moreover, a reduced left ventricular ejection fraction [21] and a normal renal function [25] reported in SV129 mice could also limit the consequence of eventually increased peripheral vascular resistances on BP.…”

Section: Discussionmentioning

confidence: 99%

Vascular Function of the Mesenteric Artery Isolated from Thyroid Hormone Receptor-E Knockout Mice

Liu

Canaple²,

Gauthier³

et al. 2014

J Vasc Res

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Objective: This study evaluated the consequences of thyroid hormone receptor-E (TRE) disruption on vascular reactivity. Methods: The activity of superior mesenteric arteries isolated from TRE knockout mice generated in the SV129 background (TRE^0/0SV) or in a pure C57BL/6 background (TRE^0/0C57) was compared to that of their corresponding wild-type strains (SV129 or C57BL/6 mice). Results: The wild-type SV129 mice exhibited an impaired acetylcholine (Ach)-induced mesenteric artery relaxationcompared to C57BL/6 mice, associated with greater responses to angiotensin II (AII) and phenylephrine (PE). The disruption of TRE decreased the vascular response to sodium nitroprusside and PE in both the SV129 and C57BL/6 genetic backgrounds.Responses to Ach and AII were also blunted, but only in TRE^0/0C57 mice. The administration of 3,3′5-triiodo-L-thyronine sodium salt (T₃) elicited a vasodilatation in C57BL/6 mice even at the lowest concentration (10^-9M); a maximal relaxation of more than 50% was observed with the concentrations between 10^-9 and 10^-8M. However, the response to T₃ was nearly absent in TRE^0/0C57 mice. Conclusion: TRE is essential for the control of vascular tone, particularly in thyroid hormone-mediated relaxation. The difference in response to Ach observed between the two wild-type mice should be taken into account for interpreting the vascular responses of genetically engineered mice. i 2014 S. Karger AG, Basel

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“…A miniaturized Non-Invasive-Clearance (NIC) technique to measure kidney function in conscious mice was applied to determine GFR, as previously described [16, 17]. The NIC-Kidney device (Mannhein Pharma and Diagnostics, Mannheim, Germany) allows for transcutaneous measurement of the elimination kinetics of the fluorescent renal marker FITC-S without surgery or blood and urine collection.…”

Section: Methodsmentioning

confidence: 99%

Hydroxyproline metabolism in a mouse model of Primary Hyperoxaluria Type 3

Knight

Lowther

et al. 2015

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Diseas

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Primary Hyperoxaluria Type 3 is a recently discovered form of this autosomal recessive disease that results from mutations in the gene coding for 4-hydroxy-2-oxoglutarate aldolase (HOGA1). This enzyme is one of the 2 unique enzymes in the hydroxyproline catabolism pathway. Affected individuals have increased urinary excretions of oxalate, 4-hydroxy-L-glutamate (4-OH-Glu), 4-hydroxy-2-oxoglutarate (HOG), and 2,4-dihydroxyglutarate (DHG). While HG and HOG are precursor substrates of HOGA1 and increases in their concentrations are expected, how DHG is formed and how HOG is metabolized to DHG and to oxalate are unclear. To resolve these important questions and to provide insight into possible therapeutic avenues for treating this disease, an animal model of the disease would be invaluable. We have developed a mouse model of this disease which has null mutations in the Hoga1 gene and have characterized its phenotype. It shares many characteristics of the human disease, particularly when challenged by the inclusion of hydroxyproline in the diet. An increased oxalate excretion is not observed in the KO mice which may be consistent with the recent recognition that only a small fraction of the individuals with the genotype for HOGA deficiency develop PH.

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Reliability of Transcutaneous Measurement of Renal Function in Various Strains of Conscious Mice

Cited by 58 publications

References 25 publications

Transcutaneous Assessment of Renal Function in Conscious Rodents

Transcutaneous Assessment of Renal Function in Conscious Rodents

Vascular Function of the Mesenteric Artery Isolated from Thyroid Hormone Receptor-E Knockout Mice

Hydroxyproline metabolism in a mouse model of Primary Hyperoxaluria Type 3

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