Transcutaneous Assessment of Renal Function in Conscious Rodents

Pérez, Zeneida Herrera; Weinfurter, Stefanie; Gretz, Norbert

doi:10.3791/53767

Cited by 28 publications

(23 citation statements)

References 18 publications

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“…Assessment of glomerular filtration at baseline and on days 2, 7, and 14 after cisplatin administration was performed by administering FITC‐sinistrin into the tail vein, and detecting the fluorescence signal transcutaneously for 90 minutes using a miniaturized device (Mannheim Pharma and Diagnostic, Mannheim, Germany, http://www.medibeacon.com/). The FITC‐sinistrin t 1/2 was computed using a specifically designed software .…”

Section: Methodssupporting

confidence: 92%

Human Kidney-Derived Cells Ameliorate Acute Kidney Injury Without Engrafting into Renal Tissue

Santeramo

Pérez

Illera

et al. 2017

Stem Cells Translational Medicine

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Previous studies have suggested that CD133+ cells isolated from human kidney biopsies have the potential to ameliorate injury following intravenous (IV) administration in rodent models of kidney disease by integrating into damaged renal tissue and generating specialized renal cells. However, whether renal engraftment of CD133+ cells is a prerequisite for ameliorating injury has not yet been unequivocally resolved. Here, we have established a cisplatin‐induced nephropathy model in immunodeficient rats to assess the efficacy of CD133+ human kidney cells in restoring renal health, and to determine the fate of these cells after systemic administration. Specifically, following IV administration, we evaluated the impact of the CD133+ cells on renal function by undertaking longitudinal measurements of the glomerular filtration rate using a novel transcutaneous device. Using histological assays, we assessed whether the human kidney cells could promote renal regeneration, and if this was related to their ability to integrate into the damaged kidneys. Our results show that both CD133+ and CD133− cells improve renal function and promote renal regeneration to a similar degree. However, this was not associated with engraftment of the cells into the kidneys. Instead, after IV administration, both cell types were exclusively located in the lungs, and had disappeared by 24 hours. Our data therefore indicate that renal repair is not mediated by CD133+ cells homing to the kidneys and generating specialized renal cells. Instead, renal repair is likely to be mediated by paracrine or endocrine factors. Stem Cells Translational Medicine 2017;6:1373–1384

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Section: Methodssupporting

confidence: 92%

Human Kidney-Derived Cells Ameliorate Acute Kidney Injury Without Engrafting into Renal Tissue

Santeramo

Pérez

Illera

et al. 2017

Stem Cells Translational Medicine

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“…Animals were induced with 4% isoflurane and anesthesia was maintained at 2% until the completion of device fixation and tail vein injection. For the measurement of GFR, a transcutaneous non-invasive clearance kidney device (Mannheim Pharma & Diagnostics GmbH, Mannheim, Germany) was fixed to the depilated skin on the back of rats using a double-sided adhesive patch following the procedures described previously (Herrera Pérez et al, 2016). Fluorescein isothiocyanate-sinistrin were dissolved in physiologic saline and administered through tail-vein injection (10 mg/100 g b.wt.…”

Section: Methodsmentioning

confidence: 99%

Liraglutide Improves Renal Endothelial Function in Obese Zucker Rats on a High-Salt Diet

Sukumaran¹,

Tsuchimochi²,

Sonobe³

et al. 2019

J Pharmacol Exp Ther

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Metabolic syndrome is a common risk factor in chronic kidney disease. We investigated whether liraglutide [(LIRA), a glucagonlike peptide-1 receptor (GLP-1R) agonist] treatment improved renal vascular function and renal remodeling in male Zucker rats on a high-salt diet (6% NaCl). Zucker lean (1/1) and obese (fa/fa) rats (8 weeks old) were treated with vehicle or LIRA (0.1 mg/kg per day) for 8 weeks on a high-salt diet. The glomerular filtration rate (GFR) was measured at 0 and 8 weeks using the fluorescein isothiocyanate/sinistrin method in conscious rats. We used X-ray microangiography to measure renal arterial vessel diameter (70-350 mm) and vessel number in vivo in anesthetized rats. Renal protein expression levels of nitrotyrosine, CD-68, endothelial nitric oxide synthase (eNOS), vascular endothelial growth factor (VEGF), transforming growth factor-b1, cyclooxygenase-2, and GLP-1R were assessed by western blotting. Renal gene expressions were determined by real-time polymerase chain reaction. In contrast to vehicletreated rats, fa/fa-LIRA rats improved GFR, nitric oxide (NO)mediated vasodilation in response to acetylcholine and sodium nitroprusside in small arterial vessels (,200 mm diameter). LIRA treatment increased vessel responsivity to NO donors in comparison with vehicle treatment. Increases in the expressions of proinflammatory, profibrotic, and oxidative stress related genes in fa/fa rats relative to 1/1 were unaltered by LIRA, other than a trend toward attenuation of VCAM-1 gene expression. However, LIRA treatment increased protein expressions of eNOS (P 5 0.014) and VEGF (P 5 0.063), while reducing glomerular macrophage infiltration in comparison with vehicle-treated fa/fa rats. Low-dose LIRA treatment improved renal vascular function through amelioration of vascular dysfunction and improved NO-mediated dilation of small intrarenal arteries and arterioles and a reduction in renal inflammation. Some of the findings from this study were part of an oral presentation entitled "Liraglutide treatment improves renal vascular function in Zucker rats as visualized by microangiography" in a symposium at the American Society of Nephrology (Kidney Week) meeting, October 30 to November 5,

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“…To date, there have been 35 peer-reviewed publications in which transcutaneous GFR monitors have been used in rats and mice (a regularly updated list of journal articles and conference abstracts in which the preclinical GFR monitor was used can be found at the MediBeacon website 2 ). Transdermal GFR measurements in rats and mice has been described in a number of publications 1 3 4 5 , and a video tutorial demonstrating its use in rats has been published 6 . However, measurement in mice presents additional technical challenges.…”

Section: Introductionmentioning

confidence: 99%

Transdermal Measurement of Glomerular Filtration Rate in Mice

Scarfe

Schock-Kusch

Ressel

et al. 2018

JoVE

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Transdermal analysis of glomerular filtration rate (GFR) is an established technique that is used to assess renal function in mouse and rat models of acute kidney injury and chronic kidney disease. The measurement system consists of a miniaturized fluorescence detector that is directly attached to the skin on the back of conscious, freely moving animals, and measures the excretion kinetics of the exogenous GFR tracer, fluorescein-isothiocyanate (FITC) conjugated sinistrin (an inulin analog). This system has been described in detail in rats. However, because of their smaller size, measurement of transcutaneous GFR in mice presents additional technical challenges. In this paper we therefore provide the first detailed practical guide to the use of transdermal GFR monitors in mice based on the combined experience of three different investigators who have been performing this assay in mice over a number of years.

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Transcutaneous Assessment of Renal Function in Conscious Rodents

Cited by 28 publications

References 18 publications

Human Kidney-Derived Cells Ameliorate Acute Kidney Injury Without Engrafting into Renal Tissue

Human Kidney-Derived Cells Ameliorate Acute Kidney Injury Without Engrafting into Renal Tissue

Liraglutide Improves Renal Endothelial Function in Obese Zucker Rats on a High-Salt Diet

Transdermal Measurement of Glomerular Filtration Rate in Mice

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