Reliability of the Survival Dose Estimated from in Vitro Cytotoxicity Testing in Photodynamic Therapy

Stoykova, Elena; Sabotinov, O.; Ion, R-M.; Alexandrova, Radostina

doi:10.1080/13102818.2008.10817546

Cited by 3 publications

(2 citation statements)

References 15 publications

(13 reference statements)

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“…The in vitro studies showed that TSPP was not toxic for the cells, it concentrated preferentially in the tumor cells, and it become cytotoxic only after irradiation, it attacked only the tumor cells after laser activation (5), and it was an efficient ROS generator in vitro (38,39).…”

Section: Discussionmentioning

confidence: 99%

The dynamics of reactive oxygen species in photodynamic therapy with tetra sulfophenyl-porphyrin

Clichici

Filip²,

Daicoviciu³

et al. 2010

Acta Physiologica Hungarica

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Photodynamic therapy (PDT) is a promising therapy especially in skin cancer, using the systemic administration of a photosensitizer (PS), followed by the local irradiation of the tumor with visible light. The antitumor effects of PDT are determined especially by the generation of cytotoxic reactive oxygen species (ROS). The 5,10,15,20-tetra-sulfo-phenyl-porphyrin (TSPP) is a synthetic photosensitizer, which proved its efficiency in in vitro studies. Our study evaluates the effects of PDT with TSPP upon the tumor levels of ROS and upon the metalloproteinases 2 (MMP2) activities on Wistar male rats bearing 256 Walker carcinosarcoma in correlation with the accumulation of PS in the tumor and with the intratumor histological alterations. The evaluations were performed dynamically, at 3 hours, 6 hours, 24 hours and 14 days after the PDT with TSPP. Our results emphasize that 24 hours after the PDT with TSPP, the ROS generation increases, as revealed by protein carbonyls and malondialdehyde levels and the antioxidant capacity (hydrogen donors, thiol groups) decreases in the tumor tissue. These parameters were correlated with the appearance of the histological disorders. The MMP-2 activity increases exponentially in the 24 hours-14 days post PDT interval. PDT with TSPP offers, in vivo , consistent results regarding ROS generation, MMP2 activation and cytotoxic capacity.

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Section: Discussionmentioning

confidence: 99%

The dynamics of reactive oxygen species in photodynamic therapy with tetra sulfophenyl-porphyrin

Clichici

Filip²,

Daicoviciu³

et al. 2010

Acta Physiologica Hungarica

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“…Re(tying) the knot -how the deleterious results of double-strand breaks could be repaired by introducing yet more double-strand breaks Modern research and medicine already possess various tools to assess capacity for DnA repair and, sometimes, to intervene where this is possible (8,16,17,18,20,54,86,99). In vitro assays for the rate of repair of double-strand breaks have been developed in order to be used in testing for cancer susceptibility and radiosensitivity (8,85,88).…”

Section: Fixing It the Wrong Way -Aberrant Repair Of Free Dna Ends Anmentioning

confidence: 99%

On a Break with the X: The Role of Repair of Double-Stranded DNA Breaks in X-Linked Disease

Caris

Cecceroni

Tummala

2012

Biotechnology & Biotechnological Equipment

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Self-assembled composite microparticles with surface protrudent porphyrin nanoparticles enhance cellular uptake and photodynamic therapy

Zhang

Qin

et al. 2017

Mater. Horiz.

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Reliability of the Survival Dose Estimated from in Vitro Cytotoxicity Testing in Photodynamic Therapy

Cited by 3 publications

References 15 publications

The dynamics of reactive oxygen species in photodynamic therapy with tetra sulfophenyl-porphyrin

The dynamics of reactive oxygen species in photodynamic therapy with tetra sulfophenyl-porphyrin

On a Break with the X: The Role of Repair of Double-Stranded DNA Breaks in X-Linked Disease

Self-assembled composite microparticles with surface protrudent porphyrin nanoparticles enhance cellular uptake and photodynamic therapy

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