This paper presented a new carrier board attachment method for pixellated CdZnTe (CZT) radiation detectors by using a special type of anisotropic conductive film (ACF) based on micro-wires. This ACF has very small pitch, high vertical electrical conductivity, and strong mechanical strength. It was found to be suitable for pixellated CZT detector assembly by optimizing detector fabrication processes and attachment conditions. ACF attached detector modules showed excellent spectra responses. Long-term stability and reliability tests on these detectors showed promising results. This ACF attachment technology had been successfully used for pixellated CZT detectors with various physical dimensions and anode pixel patterns.