Chronic suppurative otitis media was diagnosed in a 30-year old female with left ear pain and ear discharge lasting since 5 years intermittently. Lactose-negative, oxidase-negative, Gram-negative small bacilli grown as flat and gray colonies with smooth margins were identified as Kerstersia gyiorum by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and 16S rRNA analysis. Minimum inhibitory concentration values were determined by broth microdilution method (ciprofloxacin >2 μg/mL, ertapenem <0.25 μg/mL, imipenem 0.5 μg/mL, ceftazidime 2 μg/mL, amikacin ≤4 μg/mL, ceftriaxone ≤0.5 μg/mL, gentamicin 2 μg/mL and tigecycline 1 μg/mL). It was learned that her clinical findings resolved by oral amoxycillin-clavulanic acid and topical ciprofloxacin given empirically. Klimik Dergisi 2017; 30(3): 158-60.