Reliability and validity of FluoreCam for white‐spot lesion detection: An in vitro study

Abstract

Aim: In the present study, we tested the reliability and validity of a new light fluorescence device, the FluoreCam. Methods:Twenty-five human teeth were sectioned mesiodistally into halves. Group 1 (n=30) included specimens with either sound enamel or natural white-spot lesions (WSL). Group 2 (n=20) included specimens with sound enamel used to create artificial WSL. Using the FluoreCam, baseline scans of enamel surfaces were obtained under standardized conditions. Group1 was scanned again the next day. Group 2 specimens were covered with an acid-resistant nail polish, leaving a 2 × 6-mm enamel window exposed, immersed in Queiroz-solution (64 hours, 37°C/pH5 with agitation), and then scanned again. Random error of the repeated measurements (reliability) was determined using method errors and intraclass correlations (ICC). Systematic error and the ability to detect demineralization (validity) were tested using Wilcoxon signed-rank test.

Comparative study of two chemical protocols for creating white spot lesions: An in vitro FluoreCam evaluation

“…Because demineralized enamel emits less intensity of light than sound enamel, both intensity and impact of the demineralized lesion are expressed as negative values. FluoreCam has been shown to be highly reliable under constant conditions in vitro, with the intraclass correlation ranging from 0.87 to 0.95 . FluoreCam has also been used to monitor enamel demineralization and remineralization over time …”

White spot lesions: Does etching really matter?

“…The teeth were stored in 0.1% thymol solution. Assuming an effect size of 1.36, which was based on a previous study, a sample size of 26 per group was necessary to achieve a type I error rate of α=.001 and a power of 90%. An extra specimen was added to each group to compensate for potential loss or damage during the experiment.…”

Preventive effects of carbon dioxide laser and casein phosphopeptide amorphous calcium phosphate fluoride varnish on enamel demineralization: A comparative, in vitro study

“…No previous studies have compared the anticariogenic effect of fluoride varnish containing CPP‐ACP and 10.6 μm CO 2 laser using the FluoreCam (DARZA, Noblesville, IN, USA). The FluoreCam system is a new optical detection method designed for early WSL detection and is one of the most advanced and reliable devices . Based on the autofluorescence phenomenon of enamel, the FluoreCam system depicts demineralized areas as being darker than the surrounding healthy enamel and provides three outputs: area of demineralized enamel, intensity (light‐intensity loss), and impact of demineralization (product of intensity and area).…”