The aim of this research is to explore the forms of strengthening local wisdom in the Special Region of Yogyakarta that are implemented in higher education institutions based on the Peraturan Daerah Istimewa (PERDAIS) DIY No. 3 of 2017. The research method used is normative juridical research through a statute approach. The data analysis is qualitative descriptive, involving data reduction, data classification, data interpretation, data display, and drawing conclusions. The research findings show that the strengthening of local wisdom in the Special Region of Yogyakarta is implemented in several higher education institutions in DIY through the Tri Dharma of Higher Education. The Peraturan Daerah Istimewa (PERDAIS) DIY No. 3 of 2017 is supported by Law No. 12 of 2012 concerning Higher Education, Regional Regulation DIY No. 4 of 2011 concerning Cultural Values in Yogyakarta, and Regional Regulation DIY No. 5 of 2011 concerning the Management and Implementation of Culture-Based Education. The strengthening of local wisdom in DIY at higher education institutions is carried out through the dissemination of educational and teaching methods both explicitly and implicitly in several courses in study programs, as well as through Study Centers at DIY higher education institutions.