Relevance of zinc in human sperm flagella and its relation to motility

Henkel, Ralf; Bittner, Jörg; Weber, R.; Hüther, Friedrich; Miska, W.

doi:10.1016/s0015-0282(99)00141-7

Cited by 99 publications

(71 citation statements)

References 21 publications

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“…Excessive binding of zinc to sperm tails has been postulated as the mechanism [10,12,32,34]. Others have reported that the zinc concentration of the sperm tail is negatively correlated with sperm motility and velocity [35].…”

Section: Discussionmentioning

confidence: 99%

Relationship between seminal plasma zinc concentration and spermatozoa–zona pellucida binding and the ZP-induced acrosome reaction in subfertile men

Liu¹,

Sie²,

Zhao³

et al. 2009

Asian J Androl

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The aim of this study was to determine the relationship between seminal zinc concentration and spermatozoazona pellucida (ZP) binding and the ZP-induced acrosome reaction (ZPIAR) in subfertile men. Semen analyses and seminal zinc concentration assessments were carried out according to the World Health Organization manual for 458 subfertile men. A spermatozoa-ZP interaction test was carried out by incubating 2 × 10 6 motile spermatozoa with a group of four unfertilized oocytes obtained from a clinical in vitro fertilization programme. After 2 h of incubation, the number of spermatozoa bound per ZP and the ZPIAR of ZP-bound spermatozoa were examined. The effect of adding 0.5 mmol L -1 zinc to the media on the ZPIAR of spermatozoa from normozoospermic men was also tested in vitro. Seminal zinc concentration positively correlated with sperm count and duration of abstinence, but negatively correlated with semen volume. On analysis of data from all participants, both spermatozoa-ZP binding and the ZPI-AR were significantly correlated with sperm motility and normal morphology, but not with seminal zinc concentration. However, in men with normozoospermic semen, the seminal zinc concentration was significantly higher in men with defective ZPIAR ( < 16%) than in those with normal ZPIAR ( ≥ 16% ) (P < 0.01). The addition of 0.5 mmol L -1 zinc to the culture media had no effect on spermatozoa-ZP binding, but significantly reduced the ZPIAR in vitro (P < 0.001).In conclusion, seminal zinc concentration is correlated with sperm count and the duration of abstinence in subfertile men. In men with normozoospermic semen, high seminal zinc concentration may have an adverse effect on the ZPIAR.

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Section: Discussionmentioning

confidence: 99%

Relationship between seminal plasma zinc concentration and spermatozoa–zona pellucida binding and the ZP-induced acrosome reaction in subfertile men

Liu¹,

Sie²,

Zhao³

et al. 2009

Asian J Androl

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“…A diminuição do percentual de espermatozóides móveis, observada em 12 das 20 publicações 7,12,13,[15][16][17][18][19][20][21][22][23] , foi mais freqüente, sendo mais evidente quando houve controle no período de abstinência. A média de redução da motilidade anual variou de 0,3 13 a 0,7% 7 .…”

Section: Discussionunclassified

Interferência da idade sobre a qualidade seminal

Cavalcante¹,

Rocha²,

Dias³

et al. 2008

Rev. Bras. Ginecol. Obstet.

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XLSTAT (p<0,05). RESULTADOS: os indivíduos estudados tinham média de idade de 37±7,9 anos, com um volume seminal médio de 3±1,4 mL, uma concentração espermática de 61,4±66,4 espermatozóides por mL de sêmen, motilidade progressiva de 44,7±19,4% do total de espermatozóides e morfologia normal de 11,2±6,6% dos espermatozóides. As médias de idade entre os grupos foram semelhantes, exceto a média dos indivíduos com hipoespermia, que foi significativamente maior que a dos homens com normoespermia (39,6±10,3 versus 36,5±7,3, p=0,001). CONCLUSÕES: a idade interfere, de forma inversamente proporcional, sobre o volume do ejaculado, porém não exerce influência na concentração, motilidade e morfologia espermática.Abstract PURPOSE: to evaluate the influence of age on the quality of semen in men submitted to spermatic analysis in a human reproduction service, in cases of conjugal infertility. METHODS: a retrospective study in which the spermiograms of all men in process of investigation for conjugal infertility in a service of assisted reproduction in the Northeast of Brazil were evaluated from September 2002 to December 2004. A number of 531 individuals submitted to 531 spermatic evaluations were included in the study. The following parameters have been analyzed: spermatic volume, concentration, motility and morphology. The men under investigation have been divided in groups, according to the results obtained in each of the variables studied. Seminal volume groups were divided in: hypospermia, normospermia and hyperspermia. Spermatic concentration groups were divided in: azoospermia, oligospermia, normospermia and polyspermia. Motility groups were divided in: normal motility and asthenospermia. Morphology groups were divided in: normal morphology and teratospermia. The t test has been used to compare the average age of patients in groups with normal and in groups with altered parameters. The program XLSTAT (p<0.05) has been used for the statistical analysis. RESULTS: the individuals studied presented an average of 37±7.9 years old, with an average of seminal volume of 3±1.4 mL, a spermatic concentration of 61.4±66.4 spermatozoids by mL of semen, a progressive motility of 44.7±19.4% of the total of spermatozoids and normal morphology of 11.2±6.6% of the spermatozoids.

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“…Ultrastructural observations of the present study exhibited several sperm defects such as tail-elements with superfluous cytoplasm and degeneration in mitochondrial sheath which further supported that zinc deficiency affects sperm motility. Henkel et al [1999] reported the importance of zinc during epididymal sperm maturation for functional competence of the outer dense fibers and, therefore, motility. A low content of nuclear zinc would impair the structural stability of chromatin and thereby increase the vulnerability of the male genome [Kvist et al 1988].…”

Section: Zinc Deficiency and Testicular Apoptosis 237mentioning

confidence: 99%