Relationship between seminal plasma zinc concentration and spermatozoa–zona pellucida binding and the ZP-induced acrosome reaction in subfertile men

Liu, Deyi; Sie, Boon-Shih; Zhao, Shuo; Agresta, Franca; Baker, HW Gordon

doi:10.1038/aja.2009.23

Cited by 41 publications

(39 citation statements)

References 41 publications

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“…There are reports in the literature indicating that presence of zinc in culture medium can inhibit capacitation and acrosome reaction [33,[39][40][41]. On the contrary, the present Fig.…”

Section: Discussioncontrasting

confidence: 54%

Addition of zinc to human ejaculate prior to cryopreservation prevents freeze-thaw-induced DNA damage and preserves sperm function

Kotdawala

Kumar

Salian

et al. 2012

J Assist Reprod Genet

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Purpose To study the effect of addition of zinc to human semen sample prior to cryopreservation on post-thaw sperm quality and function. Methods Semen samples were collected from men attending university infertility clinic for semen analysis (n0109). Liquefied semen samples were cryopreserved in glycerol-egg yolkcitrate medium with or without the prior addition of zinc (100 μM) and stored in liquid nitrogen. After 10 days, the semen samples were thawed to assess the outcome. Sperm motility, DNA integrity, mitochondrial potential and the ability of spermatozoa to undergo capacitation and acrosome reaction was assessed in post-thaw samples. Results Semen samples cryopreserved after addition of zinc had a significantly higher percentage of sperm with intact DNA (p<0.001), mitochondrial function (p<0.001) and progressive motility (p<0.01) compared to the semen samples cryopreserved without zinc supplementation. Apart from this, ability to undergo capacitation and acrosome reaction in vitro was significantly higher in semen samples cryopreserved with zinc (p<0.0001 and p<0.001 respectively).Conclusions Addition of zinc to semen samples prior to cryopreservation helps in preventing the freeze-thaw-induced sperm DNA damage and loss of sperm function.

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“…There are reports in the literature indicating that presence of zinc in culture medium can inhibit capacitation and acrosome reaction [33,[39][40][41]. On the contrary, the present Fig.…”

Section: Discussioncontrasting

confidence: 54%

Addition of zinc to human ejaculate prior to cryopreservation prevents freeze-thaw-induced DNA damage and preserves sperm function

Kotdawala

Kumar

Salian

et al. 2012

J Assist Reprod Genet

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“…In the VNS group, observation of an extracellular matrix organization function is indicative of a maintained normal semen function in these adolescents (Pilch & Mann, ), probably through zinc metabolism pathways (Kumar et al ., ; Liu et al ., ). A previous study from our group demonstrated that extracellular matrix proteins, which have been shown to bind to spermatozoa through epididymosomes transfer (D'Amours et al ., ) may be associated to high sperm DNA fragmentation, when quantified in extracted sperm proteins (Intasqui et al ., ).…”

Section: Discussionmentioning

confidence: 97%

Determination of testicular function in adolescents with varicocoele – a proteomics approach

et al. 2016

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SUMMARYThe goal of this study was to determine seminal plasma biomarkers of testicular function in adolescents with varicocoele and to verify enriched gene ontology terms associated to these differential proteomes. An observational study was carried out in an academic research environment. A total of 77 adolescent patients were recruited from a local public school, of which 23 were without varicocoele and with normal semen analysis (control group), 37 were with varicocoele and normal semen (VNS) parameters, and 17 were with varicocoele and altered semen (VAS) parameters. Two semen collections were provided with a 1-week interval, after 2-5 days of ejaculatory abstinence. Seminal plasma proteins were identified and quantified utilizing a label-free shotgun proteomics approach, generating (i) proteins differentially expressed in each group (control, VNS, and VAS) and putative biomarkers using multivariate statistics followed by discriminant analysis. Confirmatory analysis was performed for two proteins by western blotting. Enriched biological processes and molecular functions were determined using gene ontology analysis. In total, 541 proteins were identified and quantified: 108 exclusive or overexpressed in controls, 26 in the VNS group, and 13 in the VAS group. The suggested biomarkers are Cab45/SDF4 (Q9BRK5), protein lefty-1 (O75610), DNase I (P24855), PAP2-alpha (O14494), IBP-7 (Q16270), HDC (P01860), and CRISP-3 (P54108). Western blotting results showed that Cab45 was significantly underexpressed in both varicocoele groups, and CRISP-3 was significantly overexpressed in seminal plasma of adolescents with VAS. In conclusion, specific biomarkers of spermatogenesis and homeostasis are observed in adolescents without varicocoele, and the presence of a palpable varicocoele progressively shifts these adolescents toward initially an immune response, and finally toward a chronic inflammatory profile. This shift is accompanied by decreased semen quality.

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“…Selenium plays an active role in sperm DNA compaction (Hadaszadeh & Beggs, ). In addition, zinc deficiencies in men can inhibit angiotensin‐converting enzyme activity in Leydig cells, which consequently result in testosterone depression and the impediment of spermatogenesis (Liu et al ., ). However, a minimum concentration of ROS is needed in many organs, especially the reproductive organs, as ROS is critical in regulating the onset of hyperactivation, spermatozoa capacitation and acrosome reaction.…”

Section: Discussionmentioning

confidence: 97%

Effect of metformin therapy and dietary supplements on semen parameters in hyperinsulinaemic males

Bosman¹,

Esterhuizen²,

Rodrigues³

et al. 2014

Andrologia

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SummaryPrevious reports indicated that hyperinsulinaemic men may exhibit a higher percentage of poorly compacted DNA in their spermatozoa and less success in an IVF programme (Andrologia, 45, 2003, 18; Andrologia, 2014 Andrologia, , doi: 10.1111. The aim of this study was to investigate the effect of metformin (Glucophage ® ) and antioxidant treatment (StaminoGro ® ) on the semen parameters of hyperinsulinaemic men. Nineteen hyperinsulinaemic male patients were treated for 3 months with metformin alone (Group A), and fifteen patients used metformin in combination with the nutritional supplement (Group B). Combined data of the two groups (pre-and post-treatment) differ significantly regarding sperm morphology (P = 0.0003) and CMA 3 (P < 0.0001) values. The improvement in sperm morphology after treatment was similar for the two respective groups (P < 0.05). The morphological normal sperm forms increased from the mean percentage of 3.9 to 5.5% and from 4.2 to 5.5% for Group A and B respectively. Where a combination of metformin and the supplement were used (Group B), the combination treatment proved to be superior in obtaining enhanced chromatin packaging quality although not statistically significant (P = 0.5929) when compared with the metformin (Group A) group. The chromatin packaging quality in Group B improved with 10% while the improvement in Group A was approximately 8.3%. Therefore, infertile hyperinsulinaemic men can benefit from metformin treatment and should be advised on the use of nutritional supplements with antioxidant properties.

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Relationship between seminal plasma zinc concentration and spermatozoa–zona pellucida binding and the ZP-induced acrosome reaction in subfertile men

Cited by 41 publications

References 41 publications

Addition of zinc to human ejaculate prior to cryopreservation prevents freeze-thaw-induced DNA damage and preserves sperm function

Addition of zinc to human ejaculate prior to cryopreservation prevents freeze-thaw-induced DNA damage and preserves sperm function

Determination of testicular function in adolescents with varicocoele – a proteomics approach

Effect of metformin therapy and dietary supplements on semen parameters in hyperinsulinaemic males

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