Relevance of the capacity of phosphorylated fructose to scavenge the hydroxyl radical

Spasojević, Ivan; Mojović, Miloš; Blagojević, Duško; Spasić, Snežana; Jones, David R.; Nikolić‐Kokić, Aleksandra; Spasić, Mihajlo

doi:10.1016/j.carres.2008.09.025

Cited by 57 publications

(53 citation statements)

References 25 publications

(20 reference statements)

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“…The scavenging capacity of PEG-HCCs was evaluated by EPR with the spin trap 5-(diethoxyphosphoryl)-5-methylpyrrole-N-oxide (DEPMPO). DEPMPO produces relatively stable paramagnetic adducts upon reaction with • OH (3,4,28,29) and O 2…”

Section: Significancementioning

confidence: 99%

“…Recombinant human eNOS ox was expressed in Escherichia coli [tetrahydrobiopterin (BH 4 ) deficient] and purified as described previously (17,44). Ferrous anaerobic eNOS ox (10 μM) in 0.1 M phosphate buffer with 0.1 M NaCl and 10% (vol/vol) glycerol (pH 7.7) was prepared by anaerobic titration with a minimal amount of dithionite and then mixed with oxygenated buffer containing 15 μM of ferric cytochrome c alone (as a control) or with different amounts of PEG-HCCs or SOD in an anaerobic stopped flow (SX-18MV; Applied Photophysics) (17).…”

Section: Rfq Epr Kinetic Measurements For Omentioning

confidence: 99%

“…These ROS are necessary for the signaling pathways in biological processes (3,4) such as cell migration, circadian rhythm, stem cell proliferation, and neurogenesis (5). In healthy systems, ROS are efficiently regulated by the defensive enzymes superoxide dismutase (SOD) and catalase, and by antioxidants such as glutathione, vitamin A, ascorbic acid, uric acid, hydroquinones, and vitamin E (6).…”

mentioning

confidence: 99%

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Highly efficient conversion of superoxide to oxygen using hydrophilic carbon clusters

Samuel¹,

Marcano

Berka

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

175

179

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Many diseases are associated with oxidative stress, which occurs when the production of reactive oxygen species (ROS) overwhelms the scavenging ability of an organism. Here, we evaluated the carbon nanoparticle antioxidant properties of poly(ethylene glycolated) hydrophilic carbon clusters (PEG-HCCs) by electron paramagnetic resonance (EPR) spectroscopy, oxygen electrode, and spectrophotometric assays. These carbon nanoparticles have 1 equivalent of stable radical and showed superoxide (O 2•− ) dismutase-like properties yet were inert to nitric oxide (NO • ) as well as peroxynitrite (ONOO − ). Thus, PEG-HCCs can act as selective antioxidants that do not require regeneration by enzymes. Our steadystate kinetic assay using KO 2 and direct freeze-trap EPR to follow its decay removed the rate-limiting substrate provision, thus enabling determination of the remarkable intrinsic turnover numbers of O 2 •− to O 2 by PEG-HCCs at >20,000 s −1 . The major products of this catalytic turnover are O 2 and H 2 O 2 , making the PEG-HCCs a biomimetic superoxide dismutase.superoxide | antioxidant | carbon nanoparticles | hydrophilic carbon clusters | superoxide dismutase mimetic R eactive oxygen species (ROS), such as superoxide (O 2 •− ), hydrogen peroxide (H 2 O 2 ), organic peroxides, and hydroxyl radical ( • OH), are a consequence of aerobic metabolism (1, 2). These ROS are necessary for the signaling pathways in biological processes (3, 4) such as cell migration, circadian rhythm, stem cell proliferation, and neurogenesis (5). In healthy systems, ROS are efficiently regulated by the defensive enzymes superoxide dismutase (SOD) and catalase, and by antioxidants such as glutathione, vitamin A, ascorbic acid, uric acid, hydroquinones, and vitamin E (6). When the production of ROS overwhelms the scavenging ability of the defense system, oxidative stress occurs, causing dysfunctions in cell metabolism (7)(8)(9)(10)(11)(12)(13)(14)(15)(16).In addition to ROS, reactive nitrogen species (RNS) such as nitric oxide (NO • ), nitrogen dioxide, and dinitrogen trioxide can be found in all organisms. NO • can act as an oxidizing or reducing agent depending on the environment (17), is more stable than other radicals (half-life 4-15 s) (18), and is synthesized in small amounts in vivo (17)(18)(19)(20)(21)(22). NO • is a potent vasodilator and has an important role in neurotransmission and cytoprotection (17,18,22,23). Owing to its biological importance and the low concentration found normally in vivo, it is often important to avoid alteration of NO • levels in biological systems to prevent aggravation of acute pathologies including ischemia and reperfusion.One way to treat these detrimental pathologies is to supply antioxidant molecules or particles that renormalize the disturbed oxidative condition. We recently developed a biocompatible carbon nanoparticle, the poly(ethylene glycolated) hydrophilic carbon cluster (PEG-HCC), which has shown ability to scavenge oxyradicals and protect against oxyradical damage in rodent models and thus far h...

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Section: Significancementioning

confidence: 99%

Section: Rfq Epr Kinetic Measurements For Omentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Highly efficient conversion of superoxide to oxygen using hydrophilic carbon clusters

Samuel¹,

Marcano

Berka

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

175

179

View full text Add to dashboard Cite

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“…However, short-term application of fructose demonstrated better protective action against oxidative stress than glucose (Spasojević et al, 2009a;2009b). It was suggested that any excess of fructose should be eliminated from normal diets.…”

Section: Reactive Carbonyl and Oxygen Species Are Potential Aging Andmentioning

confidence: 99%

“…It was suggested that any excess of fructose should be eliminated from normal diets. On the other hand, short-term acute application of fructose has protective effects under pathophysiological conditions related to oxidative stress (Spasojević et al, 2009a;2009b).…”

Section: Reactive Carbonyl and Oxygen Species Are Potential Aging Andmentioning

confidence: 99%

Interplay Between Oxidative and Carbonyl Stresses: Molecular Mechanisms, Biological Effects and Therapeutic Strategies of Protection

Semchyshyn¹,

Lushchak²

2012

Oxidative Stress - Molecular Mechanisms and Biological Effects

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A novel chemiluminescence system for the determination of daidzein and its hydroxyl radical‐scavenging capacity

Zhuo

Zhang

et al. 2011

Luminescence

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A novel chemiluminescence (CL) system was established for the determinations of daidzein in pharmaceutical preparations and to assess its ability to scavenge hydroxyl radicals. It was shown that a strong CL signal generated when eosin Y was mixed with Fenton reagent was decreased significantly when daidzein was added to the reaction system due to partial scavenging of the hydroxyl radicals in the solution. The extent of decrease in the CL intensity had a good stoichiometric relationship with the daidzein concentration. Based on this, we developed a new method for the determination of daidzein, using a flow-injection chemiluminescence (FI-CL) technique. Under the optimal conditions, the linear range of daidzein concentration was 8.0 × 10(-8) -3.0 × 10(-6) mol/L (R = 0.9982), with a detection limit of 9.0 × 10(-9) mol/L (S:N = 3), and the RSD was 5.8% for 1.0 × 10(-6) mol/L daidzein (n = 11). This method was successfully used in the determination of daidzein in tablets and for evaluation of the hydroxyl radical-scavenging capacity of daidzein. The possible reaction mechanism of the CL system is discussed.

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Relevance of the capacity of phosphorylated fructose to scavenge the hydroxyl radical

Cited by 57 publications

References 25 publications

Highly efficient conversion of superoxide to oxygen using hydrophilic carbon clusters

Highly efficient conversion of superoxide to oxygen using hydrophilic carbon clusters

Interplay Between Oxidative and Carbonyl Stresses: Molecular Mechanisms, Biological Effects and Therapeutic Strategies of Protection

A novel chemiluminescence system for the determination of daidzein and its hydroxyl radical‐scavenging capacity

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