Relevance of matrix metalloproteases in non-small cell lung cancer diagnosis

Abstract

BackgroundThe need for novel biomarkers that could aid in non-small cell lung cancer (NSCLC) detection, together with the relevance of Matrix Metalloproteases (MMPs) -1, -2, -7, -9 and -10 in lung tumorigenesis, prompted us to assess the diagnostic usefulness of these MMPs and the Tissue Inhibitor of Metalloproteinase (TIMP) -1 in NSCLC patients.MethodsMarkers were evaluated in an initial study cohort (19 NSCLC cases and 19 healthy controls). Those that better performed were analyzed in a larger sample including patients with benign lung diseases. Serum MMPs and TIMP-1 were determined by multiplexed immunoassays. Logistic regression was employed for multivariate analysis of biomarker combinations.ResultsMMPs and TIMP-1 were elevated in the serum of NSCLC patients compared to healthy controls. MMP-1, -7 and -9 performed at best and were further evaluated in the sample including benign pathologies, corroborating the superiority of MMP-9 in NSCLC discrimination, also at early-stage NSCLC. The optimal diagnostic value was obtained with the model including MMP-9, gender, age and smoking history, that demonstrated an AUC of 0.787, 85.54% sensitivity and 64.89% specificity.ConclusionOur results suggest that MMP-9 is a potential biomarker for NSCLC diagnosis and its combined measurement with other biomarkers could improve NSCLC detection.Electronic supplementary materialThe online version of this article (10.1186/s12885-017-3842-z) contains supplementary material, which is available to authorized users.

Blood test shows high accuracy in detecting stage I non-small cell lung cancer

“…MMP-9 is involved in basement membrane degradation that furthers tumor invasion and metastases [42]. Past studies showed that MMP-9 s are highly elevated in LC patients, especially stage III-IV [43,44]. We also observed a correlation between IL-8 and MMP-9 levels in LC patients.…”

Matrix Metalloproteinase-9 (MMP-9) as a Cancer Biomarker and MMP-9 Biosensors: Recent Advances

“…One study evaluated the level of several matrix metalloproteases (MMPs) in an initial study cohort [ 92 ]. The authors compared the serum MMP-9 level between patient group and healthy group (19 NSCLC cases and 19 healthy controls) by multiplexed immunoassays [ 92 ]. Both pro-MMP-9 and active MMP-9 were detected in the immunoassay of this study.…”

Differential expression of matrix metalloproteinases and miRNAs in the metastasis of oral squamous cell carcinoma

“…1-6), as well as the tumour and paired adjacent normal tissue samples collected from six nonmetastatic OSCC patients (No. [7][8][9][10][11][12], were used for the microarray assay. The tumour samples from another eight nonmetastatic OSCC patients (No.…”

“…Studies have shown that MMPs are involved in numerous physiopathological processes, such as tissue remodelling, embryonic development, mammary involution, bone reabsorption, and wound healing [8,9]. MMPs induced by both tumour cells and surrounding stromal cells are related to various processes associated with tumour cell proliferation, angiogenesis, neighbouring invasion and remote metastasis due to their ability to degrade ECM and alter cell migration [10,11]. Increased levels of one or several MMPs have been found in most human cancers [12].…”