Releasing bacteria from functional magnetic beads is beneficial to MALDI-TOF MS based identification

Sun, Jiejie; Shi, Haimei; Xue, Yuyan; Cheng, Wenmin; Yu, Mei; Ding, Chuan‐Fan; Xu, Fuxing; Yu, Shaoning

doi:10.1016/j.talanta.2020.121968

Cited by 12 publications

(13 citation statements)

References 26 publications

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“…Previous studies have shown that Fc-MBL@Fe 3 O 4 can capture various pathogens, including Gram-positive and Gram-negative bacteria and fungi. [27][28][29][30] This broad-spectrum superiority prompts the utility of Fc-MBL@Fe 3 O 4 in enriching bacterial mixtures in urine. The plate count method was used to calculate the bacterial capture efficiency of Fc-MBL@Fe 3 O 4 .…”

Section: Bacterial Capture Efficiency Of Fc-mbl@fe 3 O 4 In Aqueous Solution and Urinementioning

confidence: 96%

“…The synthesis of Fc-MBL@Fe 3 O 4 has been described previously. 30 Briefly, FcMBL was produced by combining the Fc region of IgG1 with human mannose-binding lectin (MBL) with the carbohydrate recognition domain (CRD) facing outward. The FcMBL protein (4 mg mL −1 ) was incubated overnight at room temperature in 7.5 mM PLP in a sodium phosphate buffer (50 mM, pH 6.4).…”

Section: Fc-mbl@fe 3 O 4 Construction and Bacterial Enrichment From Urinementioning

confidence: 99%

“…In this paper, we integrate functionalized magnetic nanoparticles and MALDI-TOF for polymicrobial cultures. Fc-MBLmodified Fe 3 O 4 can capture various pathogens in a calciumdependent manner, [27][28][29][30] and the enriched bacterial mixture from urine was tested by MALDI-TOF MS and the results were processed by an algorithm based on bacterial mixtures. The four most prevalent UTI-causing bacteria, E. coli, K. pneumoniae, S. aureus, and P. aeruginosa, were selected and paired as model samples and the bacterial mixtures were identified by MALDI-TOF MS-based algorithm profiling.…”

Section: Introductionmentioning

confidence: 99%

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Rapid identification of bacterial mixtures in urine using MALDI-TOF MS-based algorithm profiling coupled with magnetic enrichment

Cheng

Shi

Teng³

et al. 2022

Analyst

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Section: Bacterial Capture Efficiency Of Fc-mbl@fe 3 O 4 In Aqueous Solution and Urinementioning

confidence: 96%

Section: Fc-mbl@fe 3 O 4 Construction and Bacterial Enrichment From Urinementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Rapid identification of bacterial mixtures in urine using MALDI-TOF MS-based algorithm profiling coupled with magnetic enrichment

Cheng

Shi

Teng³

et al. 2022

Analyst

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“…Tryptone soybean agar (TSA) and Luria-Bertani (LB) broth were purchased from Beijing Land Bridge Technology Co., Ltd (Beijing, China). The functionalized MNPs (Fc-MBL@Fe 3 O 4 ) were synthesized as in our previous study (Sun et al, 2021). The detailed synthesis procedures and characterization results are presented in the Supporting Information.…”

Section: Chemicals and Instrumentsmentioning

confidence: 99%

Application of MALDI-TOF MS Profiling Coupled With Functionalized Magnetic Enrichment for Rapid Identification of Pathogens in a Patient With Open Fracture

et al. 2021

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Posttraumatic infections can occur in orthopedic trauma patients, especially in open fractures. Rapid and accurate identification of pathogens in orthopedic trauma is important for clinical diagnosis and antimicrobial treatment. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been successfully used for first-line identification of pathogens grown on culture plates. However, for direct analysis of liquid clinical specimens, pre-purification of the sample is necessary. Herein, we investigated the feasibility of coupling Fc-MBL@Fe3O4 enrichment with MALDI-TOF MS profiling in the identification of pathogens in liquid-cultured samples. This method is successfully used for the identification of pathogens in a patient with an open-leg fracture obtained at sea. Pathogens were enriched by Fc-MBL@Fe3O4 from briefly pre-cultured liquid media and identified by MALDI-TOF MS. We identified an opportunistic pathogen, Vibrio alginolyticus, which is uncommon in clinical orthopedic trauma infection but exists widely in the sea. Therefore, combining Fc-MBL@Fe3O4 enrichment and MALDI-TOF MS profiling has great potential for direct identification of microbes in clinical samples.

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“…Bacterial identification is a crucial component of clinical practice. The traditional method is based on the assessment of bacterial morphology, cell physiology, and biochemistry (Sun et al, 2021). While the simplicity of this method and low costs make it an attractive option, it is time-consuming and has limited clinical value due to its low positive rate (Jasson et al, 2010;Oberhettinger et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

MALDI-TOF MS Based Bacterial Antibiotics Resistance Finger Print for Diabetic Pedopathy

Sun

Lai

et al. 2022

Front. Chem.

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Diabetes mellitus has become a major global health issue. Currently, the use of antibiotics remains the best foundational strategy in the control of diabetic foot infections. However, the lack of accurate identification of pathogens and the empirical use of antibiotics at early stages of infection represents a non-targeted treatment approach with a poor curative effect that may increase the of bacterial drug resistance. Therefore, the timely identification of drug resistant bacteria is the key to increasing the efficacy of treatments for diabetic foot infections. The traditional identification method is based on bacterial morphology, cell physiology, and biochemistry. Despite the simplicity and low costs associated with this method, it is time-consuming and has limited clinical value, which delays early diagnosis and treatment. In the recent years, MALDI-TOF MS has emerged as a promising new technology in the field of clinical microbial identification. In this study, we developed a strategy for the identification of drug resistance in the diagnosis of diabetic foot infections using a combination of macro-proteomics and MALDI MS analysis. The macro-proteomics result was utilized to determine the differential proteins in the resistance group and the corresponding peptide fragments were used as the finger print in a MALDI MS analysis. This strategy was successfully used in the research of drug resistance in patients with diabetic foot infections and achieved several biomarkers that could be used as a finger print for 4 different drugs, including ceftazidime, piperacillin, levofloxacin, and tetracycline. This method can quickly confirm the drug resistance of clinical diabetic foot infections, which can help aid in the early treatment of patients.

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Releasing bacteria from functional magnetic beads is beneficial to MALDI-TOF MS based identification

Cited by 12 publications

References 26 publications

Rapid identification of bacterial mixtures in urine using MALDI-TOF MS-based algorithm profiling coupled with magnetic enrichment

Rapid identification of bacterial mixtures in urine using MALDI-TOF MS-based algorithm profiling coupled with magnetic enrichment

Application of MALDI-TOF MS Profiling Coupled With Functionalized Magnetic Enrichment for Rapid Identification of Pathogens in a Patient With Open Fracture

MALDI-TOF MS Based Bacterial Antibiotics Resistance Finger Print for Diabetic Pedopathy

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