Release of protein from highly cross-linked hydrogels of poly(ethylene glycol) diacrylate fabricated by UV polymerization

Mellott, Michael B.; Searcy, Katherine; Pishko, Michael V.

doi:10.1016/s0142-9612(00)00258-1

Cited by 356 publications

(290 citation statements)

References 34 publications

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“…Hydrogel mesh size-PEGDA hydrogel mesh size cannot be visualized using standard techniques such as scanning electron microscopy (SEM) [36]. Thus, a variety of methods to estimate PEGDA hydrogel mesh size have been developed, including correlations linking measurable quantities, such as equilibrium hydrogel swelling, to mesh size [34,37].…”

Section: 32mentioning

confidence: 99%

“…Although these correlations appear to yield reasonable mesh size estimates for relatively high weight percent PEGDA hydrogels [34,37], the predictions for lower weight percent hydrogels have been called into question. Thus, in this study, hydrogel mesh size was characterized via a series of dextran diffusion experiments based on an adaptation of the methodology of Watkins et al [38].…”

Section: 32mentioning

confidence: 99%

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Influence of hydrogel mechanical properties and mesh size on vocal fold fibroblast extracellular matrix production and phenotype

et al. 2008

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Current clinical management of vocal fold (VF) scarring produces inconsistent and often suboptimal results. Researchers are investigating a number of alternative treatments for VF lamina propria (LP) scarring, including designer implant materials for functional LP regeneration. In the present study, we investigate the effects of the initial scaffold elastic modulus and mesh size on encapsulated VF fibroblast (VFF) extracellular matrix (ECM) production toward rational scaffold design. Polyethylene glycol diacrylate (PEGDA) hydrogels were selected for this study since their material properties, including mechanical properties, mesh size, degradation rate and bioactivity, can be tightly controlled and systematically modified. Porcine VFF were encapsulated in four PEGDA hydrogels with degradation half lives of ~25 days and initial elastic compressive moduli ranging from ~30 to 100 kPa and initial mesh sizes ranging from ~9 to 27 nm. After 30 days of static culture, VFF ECM production and phenotype in each formulation was assessed biochemically and histologically. Sulfated glycosaminoglycan synthesis increased in similar degree with both increasing initial modulus and decreasing initial mesh size. In contrast, elastin production decreased with increasing initial modulus but increased with decreasing initial mesh size. Both collagen deposition and the induction of a myofibroblastic phenotype depended strongly on initial mesh size but appeared largely unaffected by variations in initial modulus. The present results indicate that scaffold mesh size warrants further investigation as a critical regulator of VFF ECM synthesis. Furthermore, this study validates a systematic and controlled approach for analyzing VFF response to scaffold properties, which should aid in rational scaffold selection/design.

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Section: 32mentioning

confidence: 99%

Section: 32mentioning

confidence: 99%

Influence of hydrogel mechanical properties and mesh size on vocal fold fibroblast extracellular matrix production and phenotype

et al. 2008

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“…HA has exposed hydroxyl and carboxyl groups that can impart a net negative charge, which would repel plasmid to enhance transport but may attract complexes and hinder diffusion. Similar observations have been noted with protein release, in which ionic interactions between the hydrogel and the protein were manipulated to obtain either retention or release [43]. Additionally, studies with cell culture substrates have indicated that DNA/PEI complexes are retained on the bio-material, with cells able to internalize the immobilized complex.…”

Section: Discussionmentioning

confidence: 52%

Non-viral vector delivery from PEG-hyaluronic acid hydrogels

Wieland

Houchin-Ray

Shea

2007

Journal of Controlled Release

121

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Hydrogels have been widely used in tissue engineering as a support for tissue formation or to deliver non-viral gene therapy vectors locally. Hydrogels that combine these functionalities can provide a fundamental tool to promote specific cellular processes leading to tissue formation. This report investigates controlled release of gene therapy vectors from hydrogels as a function of the physical properties for both the hydrogel and the vector. Hydrogels were formed by photocrosslinking acrylmodified hyaluronic acid (HA) with a 4-arm poly(ethylene glycol) (PEG) acryl. The polymer content, and relative composition of HA and PEG modulated the swelling ratio, water content, and degradation, which can influence transport of the vector through the hydrogel. All hydrogels had a water content of 94% or higher, though the water content and swelling ratio increased with a decrease in the PEG:HA ratio. Plasmids were stably incorporated into the hydrogel, with a majority of the release occurring during the initial 2 days. For incubation in buffer, the cumulative release increased with a decreasing PEG or increasing HA content, with approximately 20% to 80% released during the first week depending on the hydrogel composition. Hydrogels incubated in hyaluronidase, an enzyme that degrades HA, significantly increased plasmid release for hydrogels containing 4% PEG and 4% HA-Acryl. The encapsulation of plasmid complexed with polyethylenimine had less than 14% of the complexes released from the hydrogel both in the presence and absence of hyaluronidase. The limited release of the complexes likely results from the complex size and interactions between the vector and hydrogel. These studies demonstrate the dependence of non-viral vector release on the physical properties of the hydrogel and the vector, suggesting vector and hydrogel designs for maximizing localized delivery of non-viral vectors.

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“…The UV irradiance and the exposure time are important factors in converting C=C of the PEG-DA and controlling the mechanical strength of the hydrogel. 25 A higher conversion of the PEG-DA shows a better mechanical strength. The Ft-IR data showed that almost all of the C=C bonds in PEG-DA were converted to C-C bonds upon exposure of the PEG-DA to 2 W/cm 2 UV irradiance for 1 s (Fig.…”

Section: Preparation Of Hydrogel Beads Containing E Colimentioning

confidence: 99%