Release of main nutrients under the influence of enzymes in vitro conditions

Abstract

Purpose. To investigate the active effect of α-amylase, cellulase, neutral protease on the release of essential nutrients in vitro in the incubation medium. The task is to determine the effect of enzyme preparations on the in vitro decomposition of feed nutrients. Methods. In the process of the research general scientific methods were used: deduction, scientific hypothesis and abstraction. Methods of zootechnical analysis and active action of feed enzymes were used to determine the chemical composition of feed. Results. Solving the problem of improving the efficiency of digestion and assimilation of nutrients and biologically active substances of feed, clarifying the conditions, nature of processes and mechanisms that determine the intensity of digestion of feed in the digestive tract, is crucial in improving production efficiency. Studies have shown that the incubation of feed with different enzyme compositions had positive results. Due to the action of enzyme compositions including α-amylase, cellulase, neutral protease in different proportions, a positive effect of incubation of feed substrate on the content of crude protein, crude fat, crude fiber, crude ash, nitrogen-free extractives has been achieved. The nutritional value of compound feed (digestible protein, metabolic energy) after the introduction of enzyme compositions was determined by the calculation method. Conclusions. These data indicate a positive effect of different compositions of enzymes of cellulase, α-amylase and neutral protease on the level of the breakdown of essential nutrients in feed in vitro. The most effective result was found in the combination of feed with an enzyme composition in the amount of 5 g of cellulase, 1 g of α-amylase, 1 g of neutral protease, which leads to a decrease of crude fiber content by 3.1% compared to the control. Instead, the content of crude protein increased by 2.0%, crude fat – by 0.4%, crude ash – by 0.5%, BER – by 0.2% compared to the control. There are positive changes in nutritional value, digestible protein – by 9.4%, metabolic energy – by 2.9% compared to the control.