Release of Highly-Sulphated Glycosaminoglycans and Histamine from Human Basophils

Reilly, K. M.; Dawes, J.; Yap, P. L.; Barnetson, Ross StC; MacGregor, Ian

doi:10.1159/000234583

Cited by 7 publications

(1 citation statement)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Chondroitin sulphate is a natural constituent of connective tissues ( Hardingham, 1986 ), especially cartilage ( Lindahl & Hook, 1978 ). Chondroitin sulphate is also present in both rat MMC ( Seldin et al ., 1985b ; Razin et al ., 1983 ) and basophils ( Orenstein et al ., 1978 ; Reilly et al ., 1988 ; Seldin et al ., 1985b ; Metcalfe et al ., 1984 ), as well as in human CTMC ( Krilis et al ., 1992 ) and umbilical cord‐derived human mast cells ( Blom et al ., 1996 ). The inhibitory action of chondroitin sulphate on histamine release was more potent than of the ‘antiallergic’ drug cromolyn, especially at higher concentrations.…”

Section: Discussionmentioning

confidence: 99%

Chondroitin sulphate inhibits connective tissue mast cells

Theoharides

Patra

Boucher

et al. 2000

British J Pharmacology

View full text Add to dashboard Cite

1 Mast cells derive from the bone marrow and are responsible for the development of allergic and possibly in¯ammatory reactions. Mast cells are stimulated by immunoglobulin E (IgE) and speci®c antigen, but also by a number of neuropeptides such as neurotensin (NT), somatostatin or substance P (SP), to secrete numerous pro-in¯ammatory molecules that include histamine, cytokines and proteolytic enzymes. 2 Chondroitin sulphate, a major constituent of connective tissues and of mast cell secretory granules, had a dose-dependent inhibitory eect on rat peritoneal mast cell release of histamine induced by the mast cell secretagogue compound 48/80 (48/80). This inhibition was stronger than that of the clinically available mast cell`stabilizer' disodium cromoglycate (cromolyn). Inhibition by chondroitin sulphate increased with the length of preincubation and persisted after the drug was washed o, while the eect of cromolyn was limited by rapid tachyphylaxis. 3 Immunologic stimulation of histamine secretion from rat connective tissue mast cells (CTMC) was also inhibited, but this eect was weaker in umbilical cord-derived human mast cells and was absent in rat basophilic leukemia (RBL) cells which are considered homologous to mucosal mast cells (MMC). Oligo-and monosaccharides were not as eective as the polysaccharides. 4 Inhibition, documented by light and electron microscopy, involved a decrease of intracellular calcium ion levels shown by confocal microscopy and image analysis. Autoradiography at the ultrastructural level showed that chondroitin sulphate was mostly associated with plasma and perigranular membranes.5 Chondroitin sulphate appears to be a potent mast cell inhibitor of allergic and nonimmune stimulation with potential clinical implications.

show abstract

Section: Discussionmentioning

confidence: 99%

Chondroitin sulphate inhibits connective tissue mast cells

Theoharides

Patra

Boucher

et al. 2000

British J Pharmacology

View full text Add to dashboard Cite

show abstract

Ultrastructure of proteoglycans in the specific granules of guinea-pig basophilic leukocytes as demonstrated by cuprolinic blue staining

Landemore

Quillec

Izard

1995

Histochem Cell Biol

View full text Add to dashboard Cite

The ultrastructure of sulphate proteoglycans in basophil granules was examined using cytochemical procedures designed to stabilize and visualize these highly anionic macromolecules in situ. Unfixed or glutaraldehyde-prefixed guinea-pig spleen cells were submitted to fixation/staining in 2.5% glutaraldehyde, 0.2% cuprolinic blue (CB; a cationic phthalocyanin dye) and 0.2 or 0.3 M MgCl2 with or without glycosidase treatments. Abundant electron-dense precipitates were present throughout the granule matrix. The stained structures were often arranged in a quasi-crystalline typical banded pattern. Negative control basophils had no electrondense precipitates. Digestion with chondroitinase ABC destroyed the CB-positive electron-dense banded or filamentous patterns while sialidase treatment did not, but led to larger CB-positive filaments in the cytoplasm near the granules. Taking into account their high anionicity, as shown by the stability of dye binding in the presence of 0.3 M MgCl2, and their susceptibility to chondroitinase ABC, the CB-precipitates are assumed to be related to the sulphated proteoglycans previously characterized in basophil granules. The CB-positive crystalline or filamentous network of the granule matrix is also assumed to reflect the in situ location and organization of these intracellular proteoglycans and may be involved in maintaining the shape of the granule.

show abstract

Sulfated surfaces in sensitivity: An ‘on-off’ switch for anaphylaxis?

Lasser

1991

Medical Hypotheses

View full text Add to dashboard Cite

Release of Highly-Sulphated Glycosaminoglycans and Histamine from Human Basophils

Cited by 7 publications

References 15 publications

Chondroitin sulphate inhibits connective tissue mast cells

Chondroitin sulphate inhibits connective tissue mast cells

Ultrastructure of proteoglycans in the specific granules of guinea-pig basophilic leukocytes as demonstrated by cuprolinic blue staining

Sulfated surfaces in sensitivity: An ‘on-off’ switch for anaphylaxis?

Contact Info

Product

Resources

About