Release of Halophilic Extremozymes by Mechanical Cell Disruption

Abstract

Trimatostroma salinum, Wallemia ichthyophaga, Hortaea werneckii and Phaeotheca triangularis are halophilic fungi, which can thrive in a wide range of salinity. They present a source of valuable bioactive compounds, enzymes and proteins interesting for food and pharmaceutical industry. To separate enzymes from halophilic fungi cells, the mechanical method was used. Obtained results and new findings are important from the biotechnological point of view, since the separation of in the form of cocktail from halophilic fungi is interesting for industrial applications, especially for cascade reactions. Enzymes from extremophiles namely possess improved properties and can be used at harsh conditions where non-extremophilic enzymes may deactivate.

Bioprospecting Marine Fungal Enzymes-Scope and Challenges

“…Most of the halophilic fungi secrete extracellular enzymes, which facilitate efficient and easier extraction under industrial conditions compared to halophilic bacteria. These enzymes are water-soluble and adjust to lower water activity (Primožicˇ et al, 2019). The enzymes produced by halophilic fungi belong mainly to the classes of hydrolases (EC 3) and oxidoreductases (EC 1) (Śliżewska et al, 2022).…”

“…Halophilic hydrolases are the most commonly isolated enzymes from halophilic microorganisms, and they are recognized to have potential application in biotechnological processes, that demand high stability and activity in the presence of organic solvents at high salt concentrations (Flores-Gallegos et al, 2018;Primožicˇet al, 2019). Halophilic fungi are well known to produce amylases, lipases, cellulases, proteases, xylanases, pectinases, and others (Ruginescu et al, 2020;Zhang et al, 2018) that have applications as biofuel production, bioremediation, food, cosmetics, detergent, and pharmaceutical processes (Ali et al, 2014a;Amoozegar et al, 2019;González-Abradelo et al, 2019).…”