Cell disruption and permeabilization methods for obtaining yeast bioproducts

Gautério, Gabrielle Victoria; da Silva, Rhonyele Maciel; Karraz, Fellipe Chiara; Coelho, Maria Alice Zarur; Ribeiro, Bernardo Dias; Lemes, Ailton Cesar

doi:10.1016/j.clce.2023.100112

Cited by 13 publications

(6 citation statements)

References 169 publications

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“…Nevertheless, the comparison between processes is limited due to the lack of data or detailed information for comparison. Additionally, the processes are specifically developed for the extraction of specific components from the process [36].…”

Section: Composition Surface Tension and Emulsification Propertymentioning

confidence: 99%

“…The most abundant polysaccharides in yeast cell walls are α-glucans and β-glucans [76]. The last one consists of β-(1 → 3) and (1 → 6) linkages with a substantial structural diversity, corresponding to 29-64% of the cell wall [36]. The content of these carbohydrates, mostly β-glucans, is important to formulate foodstuffs with prebiotic features apart from modulating the sensorial characteristics [77].…”

Section: Characterization Of Crude Biomass and Food Ingredient 331 Ch...mentioning

confidence: 99%

“…Moreover, the use of endogenous enzymes (proteinases, glucanases, and chitinases) during autolysis makes the process cheaper than the use of commercial enzymes [34,35]. Moreover, the combination of methods can be a feasible alternative to improve the disruption of cell walls and the release of components such as mannoprotein, β-glucan, other proteins, and bioactive peptides [26,36,37]. For example, autolysis is a feasible first step due to the mild temperature, pH, time conditions, and great penetration power [34].…”

Section: Introductionmentioning

confidence: 99%

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Recovery of High-Value Compounds from Yarrowia lipolytica IMUFRJ 50682 Using Autolysis and Acid Hydrolysis

da Silva,

Ribeiro,

Lemes

et al. 2024

Processes

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This study aimed to evaluate the sequential hydrolysis of the biomass from unconventional and versatile Y. lipolytica to recover mannoproteins, carbohydrates, and other compounds as well as to determine the antioxidant activity of ultrafiltered fractions. The crude biomass underwent autolysis, and the resulting supernatant fraction was used for mannoprotein recovery via precipitation with ethanol. The precipitate obtained after autolysis underwent acid hydrolysis, and the resulting supernatant was ultrafiltered, precipitated, and characterized. The process yields were 55.5% and 46.14% for the crude biomass grown in glucose and glycerol, respectively. The mannoprotein with higher carbohydrate content (from crude biomass grown in glycerol) exhibited a higher emulsification index of 47.35% and thermal stability (60% weight loss). In contrast, the mannoprotein with higher protein content (from crude biomass grown in glucose) showed a better surface tension reduction of 44.50 mN/m. The technological properties showed that the crude biomass and the food ingredients are feasible to apply in food processing. The fractionation of the acid hydrolysis portion allowed the evaluation of the antioxidant power synergism among the components present in the hydrolysate, mostly the protein peptide chain. The sequential hydrolysis method is viable for extracting valuable products from Y. lipolytica.

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Section: Composition Surface Tension and Emulsification Propertymentioning

confidence: 99%

Section: Characterization Of Crude Biomass and Food Ingredient 331 Ch...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Recovery of High-Value Compounds from Yarrowia lipolytica IMUFRJ 50682 Using Autolysis and Acid Hydrolysis

da Silva,

Ribeiro,

Lemes

et al. 2024

Processes

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“…Methods for disrupting cells to accurately extract and quantify biomass protein content have been extensively explored for microalgae and yeast biotechnologies ( Halim et al, 2022 ; Rahman et al, 2022 ; Gautério et al, 2023 ). These cellular disruption techniques can be broadly classified into mechanical treatment (such as bead milling and homogenization), physical treatment (heating and sonication) and chemical/biological treatment (alkali, acids, and enzymes) ( Soto-Sierra et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of cell disruption methods for protein and coenzyme Q10 quantification in purple non-sulfur bacteria

Wada,

Rashid,

Wijten

et al. 2024

Front. Microbiol.

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A recent focus has been on the recovery of single-cell protein and other nutritionally valuable bioproducts, such as Coenzyme Q10 (CoQ10) from purple non-sulfur bacteria (PNSB) biomass following wastewater treatment. However, due to PNSB’s peculiar cell envelope (e.g., increased membrane cross-section for energy transduction) and relatively smaller cell size compared to well-studied microbial protein sources like yeast and microalgae, the effectiveness of common cell disruption methods for protein quantification from PNSB may differ. Thus, this study examines the efficiency of selected chemical (NaOH and EDTA), mechanical (homogenization and bead milling), physical (thermal and bath/probe sonication), and combined chemical–mechanical/physical treatment techniques on the PNSB cell lysis. PNSB biomass was recovered from the treatment of gas-to-liquid process water. Biomass protein and CoQ10 contents were quantified based on extraction efficiency. Considering single-treatment techniques, bead milling resulted in the best protein yields (p < 0.001), with the other techniques resulting in poor yields. However, the NaOH-assisted sonication (combined chemical/physical treatment technique) resulted in similar protein recovery (p = 1.00) with bead milling, with the former having a better amino acid profile. For example, close to 50% of the amino acids, such as sensitive ones like tryptophan, threonine, cystine, and methionine, were detected in higher concentrations in NaOH-assisted sonication (>10% relative difference) compared to bead-milling due to its less disruptive nature and improved solubility of amino acids in alkaline conditions. Overall, PNSB required more intensive protein extraction techniques than were reported to be effective on other single-cell organisms. NaOH was the preferred chemical for chemical-aided mechanical/physical extraction as EDTA was observed to interfere with the Lowry protein kit, resulting in significantly lower concentrations. However, EDTA was the preferred chemical agent for CoQ10 extraction and quantification. CoQ10 extraction efficiency was also suspected to be adversely influenced by pH and temperature.

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“…Bioproducts refer to a wide range of products derived from biological sources such as plants, animals, and microorganisms. These can be categorized into different types, including biofuels, bioplastics, biomaterials, and nanomaterials [19][20][21]. Bioactive compounds are diverse natural substances that can significantly support human health and body functions [22][23][24].…”

Section: Introductionmentioning

confidence: 99%

Valorization of Fermented Food Wastes and Byproducts: Bioactive and Valuable Compounds, Bioproduct Synthesis, and Applications

Faria,

Carvalho,

Conte-Junior

2023

Fermentation

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Significant amounts of fermented food waste are generated worldwide, promoting an abundance of residual biomass that can be used as raw material to extract bioactive peptides, fermentable sugars, polyphenols, and valuable compounds for synthesizing bioproducts. Therefore, generating these high-value-added products reduces the environmental impact caused by waste disposal and increases the industrial economic value of the final products. This review presents opportunities for synthesizing bioproducts and recovering bioactive compounds (employing wastes and byproducts from fermented sources) with several biological properties to support their consumption as dietary supplements that can benefit human health. Herein, the types of fermented food waste and byproducts (i.e., vegetables, bread wastes, dairy products, brewing, and winery sources), pre-treatment processes, the methods of obtaining products, the potential health benefits observed for the bioactive compounds recovered, and other technological applications of bioproducts are discussed. Therefore, there is currently a tendency to use these wastes to boost bioeconomic policies and support a circular bioeconomy approach that is focused on biorefinery concepts, biotechnology, and bioprocesses.

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Cell disruption and permeabilization methods for obtaining yeast bioproducts

Cited by 13 publications

References 169 publications

Recovery of High-Value Compounds from Yarrowia lipolytica IMUFRJ 50682 Using Autolysis and Acid Hydrolysis

Recovery of High-Value Compounds from Yarrowia lipolytica IMUFRJ 50682 Using Autolysis and Acid Hydrolysis

Evaluation of cell disruption methods for protein and coenzyme Q10 quantification in purple non-sulfur bacteria

Valorization of Fermented Food Wastes and Byproducts: Bioactive and Valuable Compounds, Bioproduct Synthesis, and Applications

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