Release of Ca
 2+
 from the Endoplasmic Reticulum Contributes to Ca
 2+
 Signaling in
 Dictyostelium discoideum

Wilczynska, Z.; Happle, Kathrin; Müller‐Taubenberger, Annette; Schlatterer, Christina; Malchow, Dieter; Fisher, Paul R.

doi:10.1128/ec.4.9.1513-1525.2005

Cited by 24 publications

(53 citation statements)

References 64 publications

(67 reference statements)

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“…To our knowledge, local transient Ca 2+ increases have not been studied in vegetative Dictyostelium cells. In developing cells, external stimuli such as cAMP or folate do cause a global cytosolic Ca 2+ increase, but the level of local Ca 2+ increases remains to be established (Schlatterer et al, 1994;Wilczynska et al, 2005). When such local Ca 2+ waves encounter a post-lysosome, the mucolipin channel might allow transfer of Ca 2+ into the post-lysosomes by a mechanism similar to the efficient transfer of Ca 2+ from the endoplasmic reticulum to neighboring mitochondria (Rizzuto et al, 1993).…”

Section: Discussionmentioning

confidence: 99%

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Mucolipin controls lysosome exocytosis in Dictyostelium

Lima

Leuba

Soldati

et al. 2012

Journal of Cell Science

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SummaryMucolipidosis type IV is a poorly understood lysosomal storage disease caused by alterations in the mucolipin lysosomal Ca 2+ channel. In this study, we generated mucolipin-knockout Dictyostelium cells, and observed that lysosome exocytosis was markedly increased in these cells compared with wild-type cells. In addition, mucolipin-knockout cells were more resistant to Ca 2+ deprivation, and the Ca 2+ concentration in their secretory lysosomes was decreased, suggesting that mucolipin transfers Ca 2+ ions from the cytosol to the lumen of secretory lysosomes. We speculate that mucolipin attenuates the fusogenic effect of local cytosolic increases in Ca 2+ by dissipating them into the lumen of lysosomal compartments.

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Section: Discussionmentioning

confidence: 99%

“…The Dictyostelium genome contains only four genes encoding putative Ca 2+ channels (mcln, pkd2, tpc2 and iplA) (Wilczynska et al, 2005). Dictyostelium mucolipin (Fig.…”

Section: Trp-ml Localizes To Secretory Post-lysosomesmentioning

confidence: 99%

Mucolipin controls lysosome exocytosis in Dictyostelium

Lima

Leuba

Soldati

et al. 2012

Journal of Cell Science

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“…This in turn requires Ca 2+ stores, with high capacity/low affinity CaBPs in their lumen, and mechanisms for Ca 2+ uptake and local release [43,44]. All these components, including primary and secondary active Ca 2+ transport mechanisms and Ca 2+ release channels (CRC) are abundant in protozoa in one or the other form, as found in Paramecium [45,46], and to some extent in Dictyostelium [47] and in choanoflagellates ( [48], this special issue). The first two genera represent the major phylogenetic lines and are frequently used for studies in cell biology, whereas choanoflagellates are currently only analysed by molecular biology, although with important predictions.…”

Section: An Evolutionary Time Scale and Diversification Of Ca 2+ Signmentioning

confidence: 97%

“…In Dictyostelium, according to sequencing data, a putative PMCA (pat1), ∼120 kDa large, is reported to lack the typical calmodulin-binding domain [75]. Later sequence data suggested the presence of such a domain in three paralogues, including patA, although for all three isoforms localisation to the ER and/or the plasmamembrane has been left open [47]. The plasmalemmal presence, however, is supported by the colocalisation with calmodulin [75].…”

Section: Ca 2+ Pumpsmentioning

confidence: 97%

The ancient roots of calcium signalling evolutionary tree

Plattner¹,

Verkhratsky²

2015

Cell Calcium

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Molecular cascades of calcium homeostasis and signalling (Ca(2+) pumps, channels, cation exchangers, and Ca(2+)-binding proteins) emerged in prokaryotes and further developed at the unicellular stage of eukaryote evolution. With progressive evolution, mechanisms of signalling became diversified reflecting multiplication and specialisation of Ca(2+)-regulated cellular activities. Recent genomic analysis of organisms from different systematic positions, combined with proteomic and functional probing invigorated expansion in our understanding of the evolution of Ca(2+) signalling. Particularly impressive is the consistent role of Ca(2+)-ATPases/pumps, calmodulin and calcineurin from very early stages of eukaryotic evolution, although with interspecies differences. Deviations in Ca(2+) handling and signalling are observed between vertebrates and flowering plants as well as between protists at the basis of the two systematic categories, Unikonta (for example choanoflagellates) and Bikonta (for example ciliates). Only the B-subunit of calcineurin, for instance, is maintained to regulate highly diversified protein kinases for stress defence in flowering plants, whereas the complete dimeric protein, in vertebrates up to humans, regulates gene transcription, immune-defence and plasticity of the brain. Calmodulin is similarly maintained throughout evolution, but in plants a calmoldulin-like domain is integrated into protein kinase molecules. The eukaryotic cell has inherited and invented many mechanisms to exploit the advantages of signalling by Ca(2+), and there is considerable overall similarity in basic processes of Ca(2+) regulation and signalling during evolution, although some details may vary.

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“…39,49 The oscillations of light scattering, in turn, have been reported to depend on Ca 2+ mechanisms, 50 including the release and re-uptake of Ca 2+ from the intracellular compartment into the extracellular space, and feedback loops between the intracellular compartment and intracellular Ca 2+ stores. 33,[51][52][53][54] In order to investigate the above issues quantitatively, we included the networks regulating the Ca 2+ flows through the extracellular space, intracellular compartment and ER in the proposed mathematical model. We also incorporated a simple dependence of the cell shape alterations on the intracellular Ca 2+ concentration.…”

Section: A New Model For Camp Oscillations In Dictyostelium Exhibits mentioning

confidence: 99%

Computational modelling suggests dynamic interactions between Ca2+, IP3 and G protein-coupled modules are key to robust Dictyostelium aggregation

et al. 2009

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Under conditions of starvation, Dictyostelium cells begin a programme of development during which they aggregate to form a multicellular structure by chemotaxis, guided by propagating waves of cyclic AMP that are relayed robustly from cell to cell. In this paper, we develop and analyse a new model for the intracellular and extracellular cAMP dependent processes that regulate Dictyostelium migration. The model allows, for the first time, a quantitative analysis of the dynamic interactions between calcium, IP 3 and G protein-dependent modules that are shown to be key to the generation of robust cAMP oscillations in Dictyostelium cells. The model provides a mechanistic explanation for the transient increase in cytosolic free Ca 2+ concentration seen in recent experiments with the application of the calmodulin inhibitor calmidazolium (R24571) to Dictyostelium cells, and also allows elucidation of the effects of varying both the conductivity of stretch-activated channels and the concentration of external phosphodiesterase on the oscillatory regime of an individual cell. A rigorous analysis of the robustness of the new model shows that interactions between the different modules significantly reduce the sensitivity of the resulting cAMP oscillations to variations in the kinetics of different Dictyostelium cells, an essential requirement for the generation of the spatially and temporally synchronised chemoattractant cAMP waves that guide Dictyostelium aggregation.

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Release of Ca ²⁺ from the Endoplasmic Reticulum Contributes to Ca ²⁺ Signaling in Dictyostelium discoideum

Cited by 24 publications

References 64 publications

Mucolipin controls lysosome exocytosis in Dictyostelium

Mucolipin controls lysosome exocytosis in Dictyostelium

The ancient roots of calcium signalling evolutionary tree

Computational modelling suggests dynamic interactions between Ca2+, IP3 and G protein-coupled modules are key to robust Dictyostelium aggregation

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Release of Ca 2+ from the Endoplasmic Reticulum Contributes to Ca 2+ Signaling in Dictyostelium discoideum

Cited by 24 publications

References 64 publications

Mucolipin controls lysosome exocytosis in Dictyostelium

Mucolipin controls lysosome exocytosis in Dictyostelium

The ancient roots of calcium signalling evolutionary tree

Computational modelling suggests dynamic interactions between Ca2+, IP3 and G protein-coupled modules are key to robust Dictyostelium aggregation

Contact Info

Product

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Release of Ca ²⁺ from the Endoplasmic Reticulum Contributes to Ca ²⁺ Signaling in Dictyostelium discoideum