Release of Arboviruses from Cells Cultivated with Low Ionic Strength Media

Matsumura, Takeo; Shiraki, Kazuko; Hotta, Susumu; Sashikata, Terumasa

doi:10.3181/00379727-141-36831

Cited by 8 publications

(6 citation statements)

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“…Several reports have dealt with chikungunya virus growth in cultured cells. In Vero and BHK-21 culture cells infected with this virus, the maturation sites of the virus were the cell surface membrane (4,5). In these sites budding process was seen clearly and precursor particle of viruses was found around the vacuoles in the cytoplasm of the cells.…”

mentioning

confidence: 96%

Multiplication of Chikungunya Virus in Salivary Glands of Aedes Albopictus (Oahu Strain) Mosquitoes: An Electron Microscopic Study

Soekiman

Matsumura

Yamanishi

1986

JJMSB

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mentioning

confidence: 96%

Multiplication of Chikungunya Virus in Salivary Glands of Aedes Albopictus (Oahu Strain) Mosquitoes: An Electron Microscopic Study

Soekiman

Matsumura

Yamanishi

1986

JJMSB

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“…No finding of the virus budding from the cell surface membrane in these regions was obtained ( Figures 3A, 3B). Precursor particles of chikungunya virus (Higashi et al, 1967;Matsumura et al, 1972) were not clearly observed in these salivary glands.…”

Section: Resultsmentioning

confidence: 74%

“…in cultured cells. In mammalian cell cultures such as Vero and BHK 21 cells, infected with this virus, the cell surface membrane was the site of the maturation process of the chikungunya virus (Higashi et al, 1967;Matsumura et al , 1972). Budding process can be seen commonly in this site and precursor particle of viruses were found underneath the cell surface membrane and around the vacuoles in the cytoplasma of the cells infected with the virus (Matsumura et al, 1972;Matsumura and Hotta, 1978).…”

Section: Several Reports Have Described Chikungunya Virus Morphogenesismentioning

confidence: 99%

Maturation of chikungunya virus in salivary glands of Indonesia strains of Aedes aegypti and Aedes albopictus mosquitoes: An electron microscopic study.

Soekiman

Matsumura

1987

Jap. J. Trop. Med. Hyg.

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Electron micrsocopic studies on the salivary glands of Aedes aegypti and Aedes albopicuts mosquitoes infected with chikungunya virus showed vacuolization in the cells of this organs and numerous chikungunya virions as crystalloid structures in intracytoplasmic area, revealing the spherical particle of 55-69 nm in diameter and the electron-dense internal structure of 41-48 nm in diameter. Virions were also found enormously in this apical cavities. No findings of virus budding from the cell surface membrane of chikungunya infected mosquito's salivary glands were obtained.

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“…The core structure, 30 nm in diameter, appears in the cytoplasm of infected cells and its envelopment takes place by budding through plasma membranes during the maturation process (11,16,22). These morphological characteristics are in common with those described for other members of alphaviruses, such as Western equine encephalitis (WEE) (17), Semliki Forest (SF) (1), and Sindbis (Sb) (3).…”

mentioning

confidence: 63%

Studies on Structural Proteins of Chikungunya Virus

Konishi

Hotta

1980

Microbiology and Immunology

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Chikungunya virus (CHIKV) was purified and subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis in discontinuous buffer systems. Three bands were revealed by staining with Coomassie blue; two of them (E1 and E2) were associated with the membrane, and one (C) with the core. Their molecular weights were estimated to be 56,000 (E1), 52,000 (E2), and 36,000 (C), irrespective of the concentration of acrylamide in the gels. The molar ratios of E1, E2, and C were almost equal when the sample buffer was Tris-HC1, whereas they were different when phosphate buffer was used.Chikungunya virus (CHIKV) is an alphavirus belonging to the Togaviridae, spherical in shape, 50-60 nm in size, and possessing a core structure surrounded by a membrane which has fine projections. The core structure, 30 nm in diameter, appears in the cytoplasm of infected cells and its envelopment takes place by budding through plasma membranes during the maturation process (11,16,22). These morphological characteristics are in common with those described for other members of alphaviruses, such as Western equine encephalitis (WEE) (17), Semliki Forest (SF) (1), and Sindbis (Sb) (3).Current knowledge of the chemical properties of CHIKV, however, is inadequate. While Igarashi et al (12) reported that CHIKV has 2 species of protein, a core protein and a hemagglutinin, the possibility that additional protein(s) can be identified should be considered, since it has been shown that SbV and SFV possess three and four structural proteins, respectively (18, 9). This paper describes experiments in which three species of CHIKV protein have been separated and characterized.

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Release of Arboviruses from Cells Cultivated with Low Ionic Strength Media

Cited by 8 publications

References 0 publications

Multiplication of Chikungunya Virus in Salivary Glands of Aedes Albopictus (Oahu Strain) Mosquitoes: An Electron Microscopic Study

Multiplication of Chikungunya Virus in Salivary Glands of Aedes Albopictus (Oahu Strain) Mosquitoes: An Electron Microscopic Study

Maturation of chikungunya virus in salivary glands of Indonesia strains of Aedes aegypti and Aedes albopictus mosquitoes: An electron microscopic study.

Studies on Structural Proteins of Chikungunya Virus

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