Relaxation Phenomena in Whey Protein Isolate and Monoglyceride Mixed Films at the Air−Water Interface

Abstract

Relaxation phenomena in a whey protein isolate (WPI) and monoglycerides (monopalmitin, monolaurin, and monoolein) mixed films at the air−water interface were studied using surface film balance and Brewster angle microscopy (BAM). Relaxation in surface area at a constant surface pressure (at 20 mN/m) or at constant area at the collapse point have been analyzed according to models for desorption, collapse, and/or organization/reorganization changes. At a constant surface pressure, the organization/reorganization change of WPI molecules in monopalmitin− and monoolein−WPI mixed films is the mechanism that controls the relaxation process. At the collapse point of the mixed film, the relaxation phenomena may be due either to nucleation and growth of critical nuclei of monoglyceride or to a complex mechanism including competition between desorption and monolayer collapse. For WPI−monolaurin mixed films the relaxation phenomena are mainly due to the irreversible loss of monolaurin molecules by desorption into the bulk aqueous phase.

Unraveling Complex Hysteresis Phenomenon in 1,2-Dipalmitoyl-sn-Glycero-3-Phosphocholine Monolayer: Insight into Factors Influencing Surface Dynamics

“…It is primarily associated with the relaxation time of molecules on a specific surface. This differs slightly from previous studies that focused on the relaxation time at the same surface pressure (isobaric) [65,66].…”

Structural and Topographical Characteristics of Adsorbed WPI and Monoglyceride Mixed Monolayers at the Air−Water Interface

“…Since the surface concentration is actually unknown for the adsorbed monolayer, the values were derived by assuming (Figure B) that the A values for adsorbed and spread monolayers were equal at the collapse point of the mixed film. , This assumption can be supported by the fact that, for protein films, the equilibrium spreading pressure (π e ), the surface pressure at the plateau for a saturated WPI adsorbed film, and the collapse pressure for adsorbed (Figure A) and spread ,, WPI monolayers are practically equal.…”