Structural and Topographical Characteristics of Adsorbed WPI and Monoglyceride Mixed Monolayers at the Air−Water Interface

Patino, Juan M. Rodrı́guez; Fernández, Marta Cejudo

doi:10.1021/la036190j

Cited by 16 publications

(50 citation statements)

References 25 publications

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“…(v) For adsorbed β-casein−monopalmitin mixed films a first-order-like phase transition was observed upon film expansion ( Figure B ) at surface pressures close to the equilibrium surface pressure of β-caseinwith a degenerated plateau in the π− A isotherm. This result suggests that the re-adsorption of previously displaced β-casein has kinetic character ( , ).…”

Section: Resultsmentioning

confidence: 90%

“…The surface pressure as a function of the trough area for β-casein + monopalmitin adsorbed mixed films (compression curves) is shown in Figure 5 A . As for pure β-casein adsorbed films, the actual π− A isotherms for β-casein + monopalmitin adsorbed mixed films were derived by assuming ( , ) that the A value for adsorbed and spread films was equal at the collapse point. This assumption can be supported by the fact that the surface pressure at the collapse point for adsorbed ( Figure B ) and spread () mixed films is practically equal to that for the pure monoglyceride.…”

Section: Resultsmentioning

confidence: 99%

“…At this point the monoglyceride (monopalmitin or monoolein) was spread at different points on the β-casein film. Further details about operational conditions have been described elsewhere ( , ). Mixtures of particular mass fractions—expressed as the mass fraction of monopalmitin, X MP , or monoolein, X MO , in the mixture—were studied.…”

Section: Methodsmentioning

confidence: 99%

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Spreading of Monoglycerides onto β-Casein Adsorbed Film. Structural and Dilatational Characteristics

Patino

Fernández

Niño

et al. 2006

J. Agric. Food Chem.

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The effect of monoglycerides (monopalmitin and monoolein) on the structural, topographical, and dilatational characteristics of betacasein adsorbed film at the air-water interface has been analyzed by means of surface pressure (pi)-area (A) isotherms, Brewster angle microscopy (BAM), and surface dilatational rheology. The static and dynamic characteristics of the mixed films depend on the interfacial composition and the surface pressure. At surface pressures lower than that for the beta-casein collapse (at the equilibrium surface pressure of the protein, pi(e)(beta-casein)) a mixed film of beta-casein and monoglyceride may exist. At higher surface pressures the collapsed beta-casein is partially displaced from the interface by monoglycerides. However, beta-casein displacement by monoglycerides is not quantitative at the monoglyceride concentrations studied in this work. The protein displacement by a monoglyceride is higher for monopalmitin than for monoolein and for spread than for adsorbed films. The viscoelastic characteristics of the mixed films were dominated by the presence of beta-casein in the mixture. Even at the higher surface pressures (at pi > pi(e)(beta-casein)) the small amounts of beta-casein collapsed residues at the interface have a significant effect on the surface dilatational properties of the mixed films. The structural, topographical, and viscoelastic characteristics of the mixed films corroborate the fact that protein displacement for monoglycerides is higher for spread than for adsorbed mixed films.

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Section: Resultsmentioning

confidence: 90%

Section: Resultsmentioning

confidence: 99%

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Spreading of Monoglycerides onto β-Casein Adsorbed Film. Structural and Dilatational Characteristics

Patino

Fernández

Niño

et al. 2006

J. Agric. Food Chem.

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“…However, as the penetration process progresses (at lower surface pressures) small nuclei of β-lactoglobulin (Figure Ad) and regions with small domains of monopalmitin (Figure Ae) over a thick sublayer of β-lactoglobulin with high reflectivity in the absence of AGC (Figure Af) can be clearly distinguished throughout the interface. The penetration of β-lactoglobulin into a monopalmitin monolayer can be also visualized at a microscopic level by the reduction of mobility in LC monopalmitin domains as the penetration process progresses, which correlates with the higher shear viscosity of β-lactoglobulin compared to monopalmitin monolayers …”

Section: Resultsmentioning

confidence: 99%

Penetration of β-Lactoglobulin into Monoglyceride Monolayers. Dynamics, Interactions, and Topography of Mixed Films

et al. 2006

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In this work we have analyzed the penetration of betalactoglobulin into a monoglyceride monolayer (monopalmitin or monoolein) spread at the air-water interface and its effects on the structural, dilatational, and topographical characteristics of mixed films. Dynamic tensiometry, surface film balance, Brewster angle microscopy (BAM), and surface dilatational rheology have been used, maintaining the temperature constant at 20 degrees C and the pH and ionic strength at 7 and 0.05 M, respectively. The initial surface pressure (mN/m) of the spread monoglyceride monolayer (pii(MONOGLYCERIDE)) at 10, 20, and the collapse point is the variable studied. Beta-lactoglobulin can penetrate into a spread monoglyceride monolayer at every surface pressure. The penetration of beta-lactoglobulin into the monoglyceride monolayer with a more condensed structure, at the collapse point of the monoglyceride, requires monoglyceride molecular loss by collapse and/or desorption. However, the structural, topographical, and dilatational characteristics of monoglyceride penetrated by beta-lactoglobulin mixed monolayers are essentially dominated by the presence of monoglyceride (either monopalmitin or monoolein) in the mixed film. In fact, monoglyceride molecules have the capacity to re-enter the monolayer after expansion and recompression of the mixed monolayer. Thus, monoglyceride molecular loss by collapse and/or desorption is reversible. The topography of the monolayer under dynamic conditions corroborates these conclusions.

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“…Nowadays, the advancements carried out in the Langmuir monolayer technique were successfully utilized in Alzheimer's disease and the surface chemistry interaction of peculiar conditions [6,8]. During the Langmuir monolayer formation, the uniform distribution occurs, due to which the colloidal film description became more comfortable for the particle size identification and structural properties characterization [2,9]. The Langmuir monolayer's interfacial properties have become an attractive source for its ability to control accurate measurements of the homogeneous monolayer at the interfaces [10,11].…”

Section: Introductionmentioning

confidence: 99%

Characterization of Monoolein Langmuir Monolayers Spread at The Salt Solution/Air Interface

Dhopte

Lad

2021

Preprint

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The Langmuir monolayer is commonly described at interfaces for an insoluble homogenous single molecular layer. Langmuir monolayers have demonstrated various issues regarding soft matters and complex fluids by forming ideal uniform two-dimensional structures over the air-water interface. This monolayer has advantages for evaluating physicochemical properties at interfaces and, for the insoluble molecules, can be applied simultaneously to the different interaction occurrences at interfaces. For this experiment, monoolein lipid was used as a spreading solvent to create a Langmuir monolayer, and five different types of salt sub-phases were applied for the physicochemical properties’ interaction studies. On the air-water interface, the surface properties of monoolein lipids were investigated for interfacial phase behaviors, using the Wilhelmy plate pressure sensor technique compression isotherm (π-A). Data and analysis were also contributed to the correspondent, precise verification of physical state behavior with the surface pressure measurements on the interfaces through the compressibility modulus and the elasticity modulus parameters on the surface. In the experiments, the interfacial activity of the monoolein lipids was found to be stable on the aqueous sub-phase, while the area per molecule over the interface did not have much impact as a sub-phase with a change in salts. The repeatability and reproducibility of tests were affirmed by the difference in the Langmuir monolayer’s particular phase transition orientation behavior and the stability of colloidal lipid dispersion. However, Langmuir monolayer formation contributes to several special groups being restructured and is found to be a more remarkable natural process for their attractive organic dynamic structural properties over the interface, but the interfacial molecular dynamics have proven to be difficult to calculate.

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Structural and Topographical Characteristics of Adsorbed WPI and Monoglyceride Mixed Monolayers at the Air−Water Interface

Cited by 16 publications

References 25 publications

Spreading of Monoglycerides onto β-Casein Adsorbed Film. Structural and Dilatational Characteristics

Spreading of Monoglycerides onto β-Casein Adsorbed Film. Structural and Dilatational Characteristics

Penetration of β-Lactoglobulin into Monoglyceride Monolayers. Dynamics, Interactions, and Topography of Mixed Films

Characterization of Monoolein Langmuir Monolayers Spread at The Salt Solution/Air Interface

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