Relative susceptibility of microsomes from lung, heart, liver, kidney, brain and testes to lipid peroxidation: Correlation with vitamin E content

Abstract

Rates of in vitro lipid peroxidation of microsomes and homogenates were found to vary widely among different tissues and species. In rats and rabbits, lung microsomes peroxidized at a 25- to 50-fold lower rate than liver, kidney, testes and brain microsomes. Heart microsomes peroxidized at a rate slightly greater than, but most similar to, lung microsomes. Comparison of tissue homogenates also revealed the unique resistance of lung and heart to lipid peroxidation. The ratio of vitamin E to peroxidizable polyunsaturated fatty acids in lung and heart microsomes was several-fold higher than in microsomes from the other tissues studied, which accounted for the relative resistance of lung and heart to lipid peroxidation. Liposomes of extracted rat lung microsomal lipid were also resistant to peroxidation and the amount of vitamin E contained in the lung lipid extract was sufficient to confer the same degree of resistance when incorporated into an equivalent amount of rat liver lipid. Higher rates of peroxidation in mouse lung microsomes relative to rabbit, rat and human lung microsomes were similarly correlated with a lower ratio of vitamin E to peroxidizable fatty acids in mouse lung microsomes. These data provide strong support for the role of vitamin E as the major cellular antioxidant, especially in the highly oxygenated tissues of heart and lung, and demonstrate the utility of the microsomal system in characterizing tissue differences in susceptibility to peroxidative membrane decomposition.

Effects of dietary vitamin E (DL-α-tocopheryl acetate) and vitamin C combination on piglets oxidative status and immune response at weaning

“…The susceptibility of muscle and liver tissue homogenates to iron-induced lipid oxidation was determined by a modification of Kronbrust and Mavis (1980) method, in which 1 mM FeSO 4 was used as the catalyst of lipid oxidation (approximately 1 mg · ml −1 buffer). Tissue homogenates were incubated at 37 °C in 40 mM Tris-malate buffer (pH 7.4) with 1 mM FeSO 4 in a total volume of 10 ml.…”

Influence of catechin (flavan-3-ol) addition to breeder quail (Coturnix coturnix japonica) diets on productivity, reproductive performance, egg quality and yolk oxidative stability

“…The effect of different incubation time (0, 30, 60, and 90 min) on egg yolk MDA concentrations was analysed according to the modified distillation method (Kara & Guclu 2012) described by Kornbrust and Mavis (1980). After storage at + 4°C for a week 15 eggs from each group were selected for the MDA analysis.…”

Effects of dietary inclusion of dehydrated lucerne and whole linseed on rabbit meat quality

“…The unsaturation index (Bordoni et al, 1999) The susceptibility of muscle tissue homogenates to iron-induced lipid oxidation was determined according to the method of Kornbrust and Mavis (1980). Homogenates were incubated at 37°C and aliquots were removed at fixed time intervals (0, 30, 60, 90 and 150 min) for measurement of 2-thiobarbituric acid-reactive substances (TBARS).…”